WSTF (BAZ1B) (NM_032408) Human Tagged ORF Clone

CAT#: RG216159

BAZ1B (tGFP-tagged) - Human bromodomain adjacent to zinc finger domain, 1B (BAZ1B)

Product Images

Circular map for RG216159

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: WSTF
• Synonyms: WBSCR9; WBSCR10; WSTF
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG216159 representing NM_032408
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCCGCTCCTGGGCCGCAAGCCCTTCCCGCTGGTGAAGCCGTTGCCCGGAGAGGAGCCGCTCTTCA
  CCATCCCGCACACTCAGGAGGCCTTCCGCACCCGGGAAGAGTATGAAGCCCGCTTGGAAAGGTACAGTGA
  GCGCATTTGGACGTGCAAGAGTACTGGAAGCAGTCAGCTAACACACAAGGAAGCCTGGGAGGAAGAACAG
  GAAGTTGCTGAGCTTTTGAAGGAGGAGTTTCCTGCCTGGTATGAGAAGCTTGTTCTGGAAATGGTTCACC
  ATAACACAGCCTCCTTAGAGAAGTTAGTAGATACTGCTTGGTTGGAGATCATGACCAAATATGCTGTGGG
  AGAAGAGTGTGACTTCGAGGTTGGGAAGGAGAAAATGCTCAAGGTGAAGATTGTGAAGATTCATCCTTTG
  GAGAAAGTGGATGAAGAGGCCACTGAGAAGAAATCTGATGGTGCCTGTGATTCTCCATCAAGTGACAAAG
  AGAACTCCAGTCAGATTGCTCAGGACCATCAGAAGAAGGAGACAGTTGTGAAAGAGGATGAAGGAAGGAG
  AGAGAGTATTAATGACAGAGCACGTAGATCGCCACGAAAACTTCCTACTTCATTAAAAAAAGGAGAAAGG
  AAATGGGCTCCTCCAAAATTTCTGCCTCACAAATATGATGTGAAACTACAAAATGAAGATAAGATCATCA
  GTAACGTGCCAGCAGACAGCTTGATTCGTACAGAGCGCCCACCAAATAAGGAGATAGTTCGATACTTTAT
  ACGGCATAATGCATTACGAGCTGGTACTGGTGAAAATGCACCTTGGGTCGTAGAAGATGAATTGGTGAAG
  AAATACTCTCTGCCCAGCAAGTTCAGTGACTTTTTACTTGATCCATACAAGTATATGACTCTCAACCCTT
  CTACTAAGAGGAAGAATACTGGATCCCCAGACAGGAAGCCCTCAAAGAAATCCAAGACAGACAACTCTTC
  TCTTAGTTCACCACTAAATCCTAAGTTATGGTGTCACGTACACTTGAAGAAGTCATTGAGTGGCTCGCCA
  CTCAAAGTGAAGAACTCAAAGAATTCCAAATCTCCTGAAGAACATCTAGAAGAAATGATGAAGATGATGT
  CGCCCAATAAGCTGCACACTAACTTTCACATTCCTAAAAAAGGCCCACCTGCCAAGAAACCAGGGAAGCA
  CAGTGACAAGCCTTTGAAGGCAAAGGGCAGAAGCAAAGGCATCCTGAATGGACAGAAATCCACAGGGAAT
  TCCAAATCTCCCAAAAAAGGACTGAAGACTCCTAAAACCAAAATGAAGCAGATGACTTTGTTGGATATGG
  CCAAAGGCACGCAGAAGATGACACGAGCCCCACGGAATTCTGGGGGTACACCTAGGACCTCTAGTAAACC
  TCATAAACATCTGCCTCCTGCAGCCCTACACCTCATTGCATACTACAAAGAAAACAAAGACAGGGAGGAC
  AAGAGGAGCGCCCTGTCCTGTGTTATCTCCAAAACAGCTCGTCTTCTCTCTAGTGAAGATAGAGCTCGTC
  TCCCAGAAGAATTGCGAAGTCTTGTTCAAAAACGCTATGAACTTCTAGAGCACAAAAAGAGGTGGGCTTC
  TATGTCTGAAGAACAACGGAAAGAATATTTGAAAAAGAAACGGGAGGAGCTGAAAAAGAAGTTGAAGGAA
  AAAGCCAAAGAACGAAGAGAGAAAGAAATGCTTGAGAGATTAGAAAAACAGAAGCGGTATGAGGACCAAG
  AGTTAACTGGCAAAAACCTTCCAGCATTCAGATTGGTGGATACCCCTGAAGGGCTGCCCAACACGCTGTT
  TGGGGATGTGGCCATGGTGGTGGAATTCTTGAGCTGTTATTCTGGGCTACTTTTACCAGATGCTCAGTAT
  CCTATTACTGCTGTGTCCCTTATGGAAGCCTTGAGTGCAGATAAGGGTGGCTTTTTATACCTTAACAGGG
  TGTTGGTCATCCTCTTACAGACCCTCCTACAAGATGAGATAGCAGAAGACTATGGTGAATTGGGAATGAA
  GCTGTCGGAAATCCCCTTGACTCTGCATTCTGTTTCAGAGCTGGTGCGGCTCTGCTTGCGCAGATCTGAT
  GTTCAGGAGGAAAGCGAGGGCTCAGACACAGATGACAATAAAGATTCAGCTGCATTTGAGGATAATGAGG
  TACAAGATGAGTTCCTAGAAAAGCTGGAGACCTCTGAATTTTTTGAGCTGACGTCAGAGGAGAAGCTACA
  GATCTTGACAGCACTGTGCCACCGGATCCTCATGACATACTCAGTGCAAGACCACATGGAGACCAGACAG
  CAGATGTCTGCAGAGTTGTGGAAGGAACGGCTTGCTGTGTTGAAGGAAGAAAATGATAAGAAGAGAGCAG
  AGAAACAGAAACGGAAAGAAATGGAAGCCAAAAATAAAGAAAATGGAAAAGTTGAGAATGGGTTAGGCAA
  AACTGATAGGAAAAAAGAAATTGTGAAGTTTGAGCCCCAAGTAGATACAGAAGCTGAAGACATGATTAGT
  GCTGTGAAGAGCAGAAGGTTGCTTGCCATTCAAGCTAAGAAGGAACGGGAAATCCAGGAAAGAGAAATGA
  AAGTGAAACTGGAACGCCAAGCTGAAGAAGAACGAATACGGAAGCACAAAGCAGCTGCTGAGAAAGCTTT
  CCAGGAAGGGATTGCCAAGGCCAAACTAGTCATGCGCAGGACTCCTATTGGCACAGATCGAAACCATAAT
  AGATACTGGCTCTTCTCAGATGAAGTTCCAGGATTATTCATTGAAAAAGGCTGGGTACATGACAGCATTG
  ACTACCGATTCAACCATCACTGCAAAGACCACACAGTCTCTGGTGATGAGGATTACTGTCCTCGCAGTAA
  GAAAGCAAACTTAGGTAAAAATGCAAGCATGAACACACAACATGGAACAGCAACAGAAGTTGCTGTAGAG
  ACAACCACACCCAAACAAGGACAGAACCTATGGTTTTTATGTGATAGTCAAAAGGAGCTGGATGAGTTGC
  TAAACTGTCTTCACCCTCAGGGAATAAGAGAAAGTCAACTTAAAGAGAGACTAGAGAAGAGGTACCAGGA
  CATTATTCACTCTATTCATCTAGCACGGAAGCCAAATTTGGGTCTAAAATCTTGTGATGGCAACCAGGAG
  CTTTTAAACTTCCTTCGTAGTGATCTCATTGAAGTTGCAACAAGGTTACAAAAAGGAGGACTTGGATATG
  TGGAAGAAACATCAGAATTTGAAGCCCGGGTCATTTCATTAGAGAAATTGAAGGATTTTGGTGAGTGTGT
  GATTGCCCTTCAGGCCAGTGTCATAAAGAAATTTCTCCAAGGCTTCATGGCTCCCAAGCAAAAGAGAAGA
  AAACTCCAAAGTGAAGATTCAGCAAAAACTGAGGAAGTGGATGAAGAGAAGAAAATGGTAGAGGAAGCAA
  AGGTTGCATCTGCACTGGAGAAATGGAAGACAGCAATCCGGGAAGCTCAGACTTTCTCCAGGATGCACGT
  GCTGCTTGGGATGCTTGATGCCTGTATCAAGTGGGATATGTCCGCAGAAAATGCTAGGTGCAAAGTTTGT
  CGAAAGAAAGGTGAGGATGACAAATTGATCTTATGTGATGAGTGTAATAAAGCCTTCCACCTGTTTTGTC
  TGAGGCCGGCCCTCTATGAAGTACCAGATGGTGAGTGGCAGTGCCCAGCTTGCCAGCCCGCTACTGCCAG
  GCGCAACTCCCGTGGCAGGAACTATACTGAAGAGTCTGCTTCTGAGGACAGTGAAGATGATGAGAGTGAT
  GAAGAGGAGGAGGAGGAAGAAGAGGAGGAGGAGGAAGAAGATTATGAGGTGGCTGGTTTGCGATTGAGAC
  CTCGAAAGACCATCCGGGGCAAGCACAGCGTCATCCCCCCTGCAGCAAGGTCAGGCCGGCGCCCGGGTAA
  GAAGCCACACTCTACCAGGAGGTCTCAGCCCAAGGCACCACCTGTGGATGATGCTGAGGTGGATGAGCTG
  GTGCTTCAGACCAAGCGGAGCTCCCGGAGGCAAAGCCTGGAGCTGCAGAAGTGTGAAGAGATCCTCCACA
  AGATCGTGAAGTACCGCTTCAGCTGGCCCTTCAGGGAGCCTGTGACCAGAGATGAGGCCGAGGACTACTA
  TGATGTGATCACGCACCCCATGGACTTTCAGACAGTGCAGAACAAATGTTCCTGTGGGAGCTACCGCTCT
  GTGCAGGAGTTTCTTACTGACATGAAGCAAGTGTTTACCAATGCTGAGGTTTACAACTGCCGTGGCAGCC
  ATGTGCTAAGCTGCATGGTGAAGACAGAACAGTGTCTAGTGGCTCTGTTGCATAAACACCTTCCTGGCCA
  CCCATATGTCCGCAGGAAGCGCAAGAAGTTTCCTGATAGGCTTGCTGAAGATGAAGGGGACAGTGAGCCA
  GAGGCCGTTGGACAGTCCAGGGGACGAAGACAGAAGAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG216159 representing NM_032408
  Red=Cloning site Green=Tags(s)
  
  MAPLLGRKPFPLVKPLPGEEPLFTIPHTQEAFRTREEYEARLERYSERIWTCKSTGSSQLTHKEAWEEEQ
  EVAELLKEEFPAWYEKLVLEMVHHNTASLEKLVDTAWLEIMTKYAVGEECDFEVGKEKMLKVKIVKIHPL
  EKVDEEATEKKSDGACDSPSSDKENSSQIAQDHQKKETVVKEDEGRRESINDRARRSPRKLPTSLKKGER
  KWAPPKFLPHKYDVKLQNEDKIISNVPADSLIRTERPPNKEIVRYFIRHNALRAGTGENAPWVVEDELVK
  KYSLPSKFSDFLLDPYKYMTLNPSTKRKNTGSPDRKPSKKSKTDNSSLSSPLNPKLWCHVHLKKSLSGSP
  LKVKNSKNSKSPEEHLEEMMKMMSPNKLHTNFHIPKKGPPAKKPGKHSDKPLKAKGRSKGILNGQKSTGN
  SKSPKKGLKTPKTKMKQMTLLDMAKGTQKMTRAPRNSGGTPRTSSKPHKHLPPAALHLIAYYKENKDRED
  KRSALSCVISKTARLLSSEDRARLPEELRSLVQKRYELLEHKKRWASMSEEQRKEYLKKKREELKKKLKE
  KAKERREKEMLERLEKQKRYEDQELTGKNLPAFRLVDTPEGLPNTLFGDVAMVVEFLSCYSGLLLPDAQY
  PITAVSLMEALSADKGGFLYLNRVLVILLQTLLQDEIAEDYGELGMKLSEIPLTLHSVSELVRLCLRRSD
  VQEESEGSDTDDNKDSAAFEDNEVQDEFLEKLETSEFFELTSEEKLQILTALCHRILMTYSVQDHMETRQ
  QMSAELWKERLAVLKEENDKKRAEKQKRKEMEAKNKENGKVENGLGKTDRKKEIVKFEPQVDTEAEDMIS
  AVKSRRLLAIQAKKEREIQEREMKVKLERQAEEERIRKHKAAAEKAFQEGIAKAKLVMRRTPIGTDRNHN
  RYWLFSDEVPGLFIEKGWVHDSIDYRFNHHCKDHTVSGDEDYCPRSKKANLGKNASMNTQHGTATEVAVE
  TTTPKQGQNLWFLCDSQKELDELLNCLHPQGIRESQLKERLEKRYQDIIHSIHLARKPNLGLKSCDGNQE
  LLNFLRSDLIEVATRLQKGGLGYVEETSEFEARVISLEKLKDFGECVIALQASVIKKFLQGFMAPKQKRR
  KLQSEDSAKTEEVDEEKKMVEEAKVASALEKWKTAIREAQTFSRMHVLLGMLDACIKWDMSAENARCKVC
  RKKGEDDKLILCDECNKAFHLFCLRPALYEVPDGEWQCPACQPATARRNSRGRNYTEESASEDSEDDESD
  EEEEEEEEEEEEEDYEVAGLRLRPRKTIRGKHSVIPPAARSGRRPGKKPHSTRRSQPKAPPVDDAEVDEL
  VLQTKRSSRRQSLELQKCEEILHKIVKYRFSWPFREPVTRDEAEDYYDVITHPMDFQTVQNKCSCGSYRS
  VQEFLTDMKQVFTNAEVYNCRGSHVLSCMVKTEQCLVALLHKHLPGHPYVRRKRKKFPDRLAEDEGDSEP
  EAVGQSRGRRQKK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032408
• ORF Size: 4449 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032408.1, NP_115784.1
• RefSeq Size: 6043 bp
• RefSeq ORF: 4452 bp
• Locus ID: 9031
• UniProt ID: Q9UIG0
• Cytogenetics: 7q11.23
• Domains: BROMO, PHD, DDT
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: This gene encodes a member of the bromodomain protein family. The bromodomain is a structural motif characteristic of proteins involved in chromatin-dependent regulation of transcription. This gene is deleted in Williams-Beuren syndrome, a developmental disorder caused by deletion of multiple genes at 7q11.23. [provided by RefSeq, Jul 2008]