RBM47 (NM_019027) Human Tagged ORF Clone

CAT#: RG215972

RBM47 (tGFP-tagged) - Human RNA binding motif protein 47 (RBM47), transcript variant 2

Product Images

Circular map for RG215972

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: RBM47
• Synonyms: NET18
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG215972 representing NM_019027
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCGCAGAGGATTCCACCGCAGCCATGAGCAGTGACTCGGCCGCCGGGTCCTCGGCCAAGGTGCCCG
  AGGGCGTGGCGGGCGCGCCCAACGAGGCAGCACTGCTGGCGCTGATGGAGCGCACGGGCTACAGCATGGT
  GCAAGAGAACGGGCAGCGCAAGTACGGCGGCCCACCGCCCGGCTGGGAGGGCCCGCACCCGCAGCGTGGC
  TGCGAGGTCTTCGTGGGCAAGATCCCGCGCGACGTGTACGAGGACGAGCTGGTGCCCGTGTTCGAGGCCG
  TGGGCCGCACCTACGAGCTGCGCCTCATGATGGACTTTGACGGCAAGAACCGCGGCTACGCCTTCGTCAT
  GTACTGCCACAAGCACGAGGCCAAGCGCGCAGTGCGTGAGCTCAACAACTACGAGATCCGCCCGGGCCGC
  CTGCTCGGCGTGTGCTGCAGCGTGGACAACTGCCGCCTCTTCATCGGCGGGATCCCCAAGATGAAGAAGC
  GCGAGGAAATCCTGGAGGAGATTGCCAAGGTCACCGAGGGCGTGCTGGACGTGATCGTCTACGCCAGCGC
  GGCCGACAAGATGAAGAACCGCGGCTTCGCCTTCGTGGAGTACGAGAGCCACCGCGCGGCTGCCATGGCT
  CGCCGCAAGCTCATGCCTGGCCGCATCCAGCTGTGGGGCCACCAGATCGCCGTGGACTGGGCCGAGCCTG
  AGATCGACGTGGACGAGGACGTGATGGAGACCGTGAAGATCCTCTACGTGCGCAACCTCATGATCGAGAC
  CACCGAGGACACCATCAAGAAGAGCTTCGGCCAGTTCAACCCCGGCTGCGTGGAGCGCGTCAAGAAGATC
  CGCGACTACGCCTTCGTGCACTTCACCAGCCGCGAGGATGCCGTGCATGCCATGAACAACCTCAACGGCA
  CTGAGCTGGAGGGCTCGTGCCTGGAGGTCACGCTGGCCAAGCCCGTGGACAAGGAGCAGTACTCGCGCTA
  CCAGAAGGCAGCCAGGGGCGGCGGCGCGGCTGAGGCAGCGCAGCAGCCCAGCTACGTGTACTCCTGCGAC
  CCCTACACACTGGCCTACTACGGCTACCCCTACAACGCGCTCATTGGGCCCAACAGGGACTACTTTGTGA
  AAGTAGCCATCCCTGCCATTGGGGCTCAGTATTCCATGTTTCCAGCAGCTCCAGCCCCTAAAATGATTGA
  AGATGGCAAAATCCACACAGTGGAGCACATGATCAGCCCCATTGCTGTGCAGCCAGACCCAGCCAGTGCT
  GCTGCCGCCGCAGCCGCGGCCGCAGCCGCCGCAGCCGCTGTCATTCCCACTGTGTCGACGCCACCACCTT
  TCCAGGGCCGCCCAATAACTCCAGTATACACGGTGGCTCCAAACGTTCAGAGAATTCCTACTGCCGGGAT
  CTACGGGGCCAGTTACGTGCCATTTGCTGCTCCAGCTACAGCCACGATCGCCACACTACAGAAGAACGCG
  GCAGCCGCGGCCGCCGTGTATGGAGGATACGCAGGCTACATACCTCAGGCCTTCCCTGCTGCTGCCATTC
  AGGTCCCCATCCCCGACGTCTACCAGACATAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG215972 representing NM_019027
  Red=Cloning site Green=Tags(s)
  
  MTAEDSTAAMSSDSAAGSSAKVPEGVAGAPNEAALLALMERTGYSMVQENGQRKYGGPPPGWEGPHPQRG
  CEVFVGKIPRDVYEDELVPVFEAVGRTYELRLMMDFDGKNRGYAFVMYCHKHEAKRAVRELNNYEIRPGR
  LLGVCCSVDNCRLFIGGIPKMKKREEILEEIAKVTEGVLDVIVYASAADKMKNRGFAFVEYESHRAAAMA
  RRKLMPGRIQLWGHQIAVDWAEPEIDVDEDVMETVKILYVRNLMIETTEDTIKKSFGQFNPGCVERVKKI
  RDYAFVHFTSREDAVHAMNNLNGTELEGSCLEVTLAKPVDKEQYSRYQKAARGGGAAEAAQQPSYVYSCD
  PYTLAYYGYPYNALIGPNRDYFVKVAIPAIGAQYSMFPAAPAPKMIEDGKIHTVEHMISPIAVQPDPASA
  AAAAAAAAAAAAAVIPTVSTPPPFQGRPITPVYTVAPNVQRIPTAGIYGASYVPFAAPATATIATLQKNA
  AAAAAVYGGYAGYIPQAFPAAAIQVPIPDVYQTY
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019027
• ORF Size: 1572 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019027.2
• RefSeq Size: 4020 bp
• RefSeq ORF: 1575 bp
• Locus ID: 54502
• UniProt ID: A0AV96
• Cytogenetics: 4p14
• Domains: RRM