LAMA3 (NM_000227) Human Tagged ORF Clone

CAT#: RG215891

LAMA3 (tGFP-tagged) - Human laminin, alpha 3 (LAMA3), transcript variant 2

Product Images

Circular map for RG215891

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: LAMA3
• Synonyms: BM600; E170; LAMNA; LOCS
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG215891 representing NM_000227
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTCCAGCAGTGAGGCGGTCAGCCTGCAGCATGGGATGGCTGTGGATCTTTGGGGCAGCCCTGGGGC
  AGTGTCTGGGCTACAGTTCACAGCAGCAAAGGGTGCCATTTCTTCAGCCTCCCGGTCAAAGTCAACTGCA
  AGCGAGTTATGTGGAGTTTAGACCCAGCCAGGGTTGTAGCCCTGGATACTATCGGGATCATAAAGGCTTG
  TATACCGGACGGTGTGTTCCCTGCAATTGCAACGGACATTCAAATCAATGCCAGGATGGCTCAGGCATAT
  GTGTTAACTGTCAGCACAACACCGCGGGAGAGCACTGTGAACGCTGCCAGGAGGGCTACTATGGCAACGC
  CGTCCACGGATCCTGCAGGGCCTGCCCATGTCCTCACACTAACAGCTTTGCCACTGGCTGTGTGGTGAAT
  GGGGGAGACGTGCGGTGCTCCTGCAAAGCTGGGTACACAGGAACACAGTGTGAAAGGTGTGCACCGGGAT
  ATTTCGGGAATCCCCAGAAATTCGGAGGTAGCTGCCAACCATGCAGTTGTAACAGCAATGGCCAGCTGGG
  CAGCTGTCATCCCCTGACTGGAGACTGCATAAACCAAGAACCCAAAGATAGCAGCCCTGCAGAAGAATGT
  GATGATTGCGACAGCTGTGTGATGACCCTCCTGAACGACCTGGCCACCATGGGCGAGCAGCTCCGCCTGG
  TCAAGTCTCAGCTGCAGGGCCTGAGTGCCAGCGCAGGGCTTCTGGAGCAGATGAGGCACATGGAGACCCA
  GGCCAAGGACCTGAGGAATCAGTTGCTCAACTACCGTTCTGCCATTTCAAATCATGGATCAAAAATAGAA
  GGCCTGGAAAGAGAACTGACTGATTTGAATCAAGAATTTGAGACTTTGCAAGAAAAGGCTCAAGTAAATT
  CCAGAAAAGCACAAACATTAAACAACAATGTTAATCGGGCAACACAAAGCGCAAAAGAACTGGATGTGAA
  GATTAAAAATGTCATCCGGAATGTGCACATTCTTTTAAAGCAGATCTCTGGGACAGATGGAGAGGGAAAC
  AACGTGCCTTCAGGTGACTTTTCCAGAGAGTGGGCTGAAGCCCAGCGCATGATGAGGGAACTGCGGAACA
  GGAACTTTGGAAAGCACCTCAGAGAAGCAGAAGCTGATAAAAGGGAGTCGCAGCTCTTGCTGAACCGGAT
  AAGGACCTGGCAGAAAACCCACCAGGGGGAGAACAATGGGCTTGCTAACAGTATCCGGGATTCTTTAAAT
  GAATACGAAGCCAAACTCAGTGACCTTCGTGCTCGGCTGCAGGAGGCAGCTGCCCAAGCCAAGCAGGCAA
  ATGGCTTGAACCAAGAAAACGAGAGAGCTTTGGGAGCCATTCAGAGACAAGTGAAAGAAATAAATTCCCT
  GCAGAGTGATTTCACCAAGTATCTAACCACTGCAGACTCATCTTTGTTGCAAACCAACATTGCGCTGCAG
  CTGATGGAGAAAAGCCAGAAGGAATATGAAAAATTAGCTGCCAGTTTAAATGAAGCAAGACAAGAACTAA
  GTGACAAAGTAAGAGAACTTTCCAGATCTGCTGGCAAAACATCCCTTGTGGAGGAGGCAGAAAAGCACGC
  GCGGTCCTTACAAGAGCTGGCAAAGCAGCTGGAAGAGATCAAGAGAAACGCCAGCGGGGATGAGCTGGTG
  CGCTGTGCTGTGGATGCCGCCACCGCCTACGAGAACATCCTCAATGCCATCAAAGCGGCCGAGGACGCAG
  CCAACAGGGCTGCCAGTGCATCTGAATCTGCCCTCCAGACAGTGATAAAGGAAGATCTGCCAAGAAAAGC
  TAAAACCCTGAGTTCCAACAGTGATAAACTGTTAAATGAAGCCAAGATGACACAAAAGAAGCTAAAGCAA
  GAAGTCAGTCCAGCTCTCAACAACCTACAGCAAACCCTGAATATTGTGACAGTTCAGAAAGAAGTGATAG
  ACACCAATCTCACAACTCTCCGAGATGGTCTTCATGGGATACAGAGAGGTGATATTGATGCTATGATCAG
  TAGTGCAAAGAGCATGGTCAGAAAGGCCAACGACATCACAGATGAGGTTCTGGATGGGCTCAACCCCATC
  CAGACAGATGTGGAAAGAATTAAGGACACCTATGGGAGGACACAGAACGAAGACTTCAAAAAGGCTCTGA
  CTGATGCAGATAACTCGGTGAATAAGTTAACCAACAAACTACCTGATCTTTGGCGCAAGATTGAAAGTAT
  CAACCAACAGCTGTTGCCCTTGGGAAACATCTCTGACAACATGGACAGAATACGAGAACTAATTCAGCAG
  GCCAGAGATGCTGCCAGTAAGGTTGCTGTCCCCATGAGGTTCAATGGTAAATCTGGAGTCGAAGTCCGAC
  TGCCAAATGACCTGGAAGATTTGAAAGGATATACATCTCTGTCCTTGTTTCTCCAAAGGCCCAACTCAAG
  AGAAAATGGGGGTACTGAGAATATGTTTGTGATGTACCTTGGAAATAAAGATGCCTCCCGGGACTACATC
  GGCATGGCAGTTGTGGATGGCCAGCTCACCTGTGTCTACAACCTGGGGGACCGTGAGGCTGAACTCCAAG
  TGGACCAGATCTTGACCAAGAGTGAGACTAAGGAGGCAGTTATGGATCGGGTGAAATTTCAGAGAATTTA
  TCAGTTTGCAAGGCTTAATTACACCAAAGGAGCCACATCCAGTAAACCAGAAACACCCGGAGTCTATGAC
  ATGGATGGTAGAAATAGCAATACACTCCTTAATTTGGATCCTGAAAATGTTGTATTTTATGTTGGAGGTT
  ACCCACCTGATTTTAAACTTCCCAGTCGACTAAGTTTCCCTCCATACAAAGGTTGTATTGAATTAGATGA
  CCTCAATGAAAATGTTCTGAGCTTGTACAACTTCAAAAAAACATTCAATCTCAACACAACTGAAGTGGAG
  CCTTGTAGAAGGAGGAAGGAAGAGTCAGACAAAAATTATTTTGAAGGTACGGGCTATGCTCGAGTTCCAA
  CTCAACCACATGCTCCCATCCCAACCTTTGGACAGACAATTCAGACCACCGTGGATAGAGGCTTGCTGTT
  CTTTGCAGAAAACGGGGATCGCTTCATATCTCTAAATATAGAAGATGGCAAGCTCATGGTGAGATACAAA
  CTGAATTCAGAGCTACCAAAAGAGAGAGGAGTTGGAGACGCCATAAACAACGGCAGAGACCATTCGATTC
  AGATCAAAATTGGAAAACTCCAAAAGCGTATGTGGATAAATGTGGACGTTCAAAACACTATAATTGATGG
  TGAAGTATTTGATTTCAGCACATATTATCTGGGAGGAATTCCAATTGCAATCAGGGAAAGATTTAACATT
  TCTACGCCTGCTTTCCGAGGCTGCATGAAAAATTTGAAGAAAACCAGTGGTGTCGTTAGATTGAATGATA
  CTGTGGGAGTAACCAAAAAGTGCTCGGAAGACTGGAAGCTTGTGCGATCTGCCTCATTCTCCAGAGGAGG
  ACAATTGAGTTTCACTGATTTGGGCTTACCACCTACTGACCACCTCCAGGCCTCATTTGGATTTCAGACC
  TTTCAACCCAGTGGCATATTATTAGATCATCAGACATGGACAAGGAACCTGCAGGTCACTCTGGAAGATG
  GTTACATTGAATTGAGCACCAGCGATAGCGGCAGCCCAATTTTTAAATCTCCACAGACGTATATGGATGG
  TTTACTGCATTATGTATCTGTAATAAGCGACAACTCTGGACTACGGCTTCTCATCGATGACCAGCTTCTG
  AGAAATAGCAAAAGGCTAAAACACATTTCAAGTTCCCGGCAGTCTCTGCGTCTGGGCGGGAGCAATTTTG
  AGGGTTGTATTAGCAATGTTTTTGTCCAGAGGTTATCACTGAGTCCTGAAGTCCTAGATTTGACCAGTAA
  CTCTCTCAAGAGAGATGTGTCCCTGGGAGGCTGCAGTTTAAACAAACCACCTTTTCTAATGTTGCTTAAA
  GGTTCTACCAGGTTTAACAAGACCAAGACTTTTCGTATCAACCAGCTGTTGCAGGACACACCAGTGGCCT
  CCCCAAGGAGCGTGAAGGTGTGGCAAGATGCTTGCTCACCACTTCCCAAGACCCAGGCCAATCATGGAGC
  CCTCCAGTTTGGGGACATTCCCACCAGCCACTTGCTATTCAAGCTTCCTCAGGAGCTGCTGAAACCCAGG
  TCACAGTTTGCTGTGGACATGCAGACAACATCCTCCAGAGGACTGGTGTTTCACACGGGCACTAAGAACT
  CCTTTATGGCTCTTTATCTTTCAAAAGGACGTCTGGTCTTTGCACTGGGGACAGATGGGAAAAAATTGAG
  GATCAAAAGCAAGGAGAAATGCAATGATGGGAAATGGCACACGGTGGTGTTTGGCCATGATGGGGAAAAG
  GGGCGCTTGGTTGTGGATGGACTGAGGGCCCGGGAGGGAAGTTTGCCTGGAAACTCCACCATCAGCATCA
  GAGCGCCAGTTTACCTGGGATCACCTCCATCAGGGAAACCAAAGAGCCTCCCCACAAACAGCTTTGTGGG
  ATGCCTGAAGAACTTTCAGCTGGATTCAAAACCCTTGTATACCCCTTCTTCAAGCTTCGGGGTGTCTTCC
  TGCTTGGGTGGTCCTTTGGAGAAAGGCATTTATTTCTCTGAAGAAGGAGGTCATGTCGTCTTGGCTCACT
  CTGTATTGTTGGGGCCAGAATTTAAGCTTGTTTTCAGCATCCGCCCAAGAAGTCTCACTGGGATCCTAAT
  ACACATCGGAAGTCAGCCCGGGAAGCACTTATGTGTTTACCTGGAGGCAGGAAAGGTCACGGCCTCTATG
  GACAGTGGGGCAGGTGGGACCTCAACGTCGGTCACACCAAAGCAGTCTCTGTGTGATGGACAGTGGCACT
  CGGTGGCAGTCACCATAAAACAACACATCCTGCACCTGGAACTGGACACAGACAGTAGCTACACAGCTGG
  ACAGATCCCCTTCCCACCTGCCAGCACTCAAGAGCCACTACACCTTGGAGGTGCTCCAGCCAATTTGACG
  ACACTGAGGATCCCTGTGTGGAAATCATTCTTTGGCTGTCTGAGGAATATTCATGTCAATCACATCCCTG
  TCCCTGTCACTGAAGCCTTGGAAGTCCAGGGGCCTGTCAGTCTGAATGGTTGTCCTGACCAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG215891 representing NM_000227
  Red=Cloning site Green=Tags(s)
  
  MPPAVRRSACSMGWLWIFGAALGQCLGYSSQQQRVPFLQPPGQSQLQASYVEFRPSQGCSPGYYRDHKGL
  YTGRCVPCNCNGHSNQCQDGSGICVNCQHNTAGEHCERCQEGYYGNAVHGSCRACPCPHTNSFATGCVVN
  GGDVRCSCKAGYTGTQCERCAPGYFGNPQKFGGSCQPCSCNSNGQLGSCHPLTGDCINQEPKDSSPAEEC
  DDCDSCVMTLLNDLATMGEQLRLVKSQLQGLSASAGLLEQMRHMETQAKDLRNQLLNYRSAISNHGSKIE
  GLERELTDLNQEFETLQEKAQVNSRKAQTLNNNVNRATQSAKELDVKIKNVIRNVHILLKQISGTDGEGN
  NVPSGDFSREWAEAQRMMRELRNRNFGKHLREAEADKRESQLLLNRIRTWQKTHQGENNGLANSIRDSLN
  EYEAKLSDLRARLQEAAAQAKQANGLNQENERALGAIQRQVKEINSLQSDFTKYLTTADSSLLQTNIALQ
  LMEKSQKEYEKLAASLNEARQELSDKVRELSRSAGKTSLVEEAEKHARSLQELAKQLEEIKRNASGDELV
  RCAVDAATAYENILNAIKAAEDAANRAASASESALQTVIKEDLPRKAKTLSSNSDKLLNEAKMTQKKLKQ
  EVSPALNNLQQTLNIVTVQKEVIDTNLTTLRDGLHGIQRGDIDAMISSAKSMVRKANDITDEVLDGLNPI
  QTDVERIKDTYGRTQNEDFKKALTDADNSVNKLTNKLPDLWRKIESINQQLLPLGNISDNMDRIRELIQQ
  ARDAASKVAVPMRFNGKSGVEVRLPNDLEDLKGYTSLSLFLQRPNSRENGGTENMFVMYLGNKDASRDYI
  GMAVVDGQLTCVYNLGDREAELQVDQILTKSETKEAVMDRVKFQRIYQFARLNYTKGATSSKPETPGVYD
  MDGRNSNTLLNLDPENVVFYVGGYPPDFKLPSRLSFPPYKGCIELDDLNENVLSLYNFKKTFNLNTTEVE
  PCRRRKEESDKNYFEGTGYARVPTQPHAPIPTFGQTIQTTVDRGLLFFAENGDRFISLNIEDGKLMVRYK
  LNSELPKERGVGDAINNGRDHSIQIKIGKLQKRMWINVDVQNTIIDGEVFDFSTYYLGGIPIAIRERFNI
  STPAFRGCMKNLKKTSGVVRLNDTVGVTKKCSEDWKLVRSASFSRGGQLSFTDLGLPPTDHLQASFGFQT
  FQPSGILLDHQTWTRNLQVTLEDGYIELSTSDSGSPIFKSPQTYMDGLLHYVSVISDNSGLRLLIDDQLL
  RNSKRLKHISSSRQSLRLGGSNFEGCISNVFVQRLSLSPEVLDLTSNSLKRDVSLGGCSLNKPPFLMLLK
  GSTRFNKTKTFRINQLLQDTPVASPRSVKVWQDACSPLPKTQANHGALQFGDIPTSHLLFKLPQELLKPR
  SQFAVDMQTTSSRGLVFHTGTKNSFMALYLSKGRLVFALGTDGKKLRIKSKEKCNDGKWHTVVFGHDGEK
  GRLVVDGLRAREGSLPGNSTISIRAPVYLGSPPSGKPKSLPTNSFVGCLKNFQLDSKPLYTPSSSFGVSS
  CLGGPLEKGIYFSEEGGHVVLAHSVLLGPEFKLVFSIRPRSLTGILIHIGSQPGKHLCVYLEAGKVTASM
  DSGAGGTSTSVTPKQSLCDGQWHSVAVTIKQHILHLELDTDSSYTAGQIPFPPASTQEPLHLGGAPANLT
  TLRIPVWKSFFGCLRNIHVNHIPVPVTEALEVQGPVSLNGCPDQ
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000227
• ORF Size: 5172 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000227.3
• RefSeq Size: 5601 bp
• RefSeq ORF: 5175 bp
• Locus ID: 3909
• UniProt ID: Q16787
• Cytogenetics: 18q11.2
• Domains: LamG, EGF_Lam
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: ECM-receptor interaction, Focal adhesion, Pathways in cancer, Small cell lung cancer
• Gene Summary: The protein encoded by this gene belongs to the laminin family of secreted molecules. Laminins are heterotrimeric molecules that consist of alpha, beta, and gamma subunits that assemble through a coiled-coil domain. Laminins are essential for formation and function of the basement membrane and have additional functions in regulating cell migration and mechanical signal transduction. This gene encodes an alpha subunit and is responsive to several epithelial-mesenchymal regulators including keratinocyte growth factor, epidermal growth factor and insulin-like growth factor. Mutations in this gene have been identified as the cause of Herlitz type junctional epidermolysis bullosa and laryngoonychocutaneous syndrome. Alternative splicing and alternative promoter usage result in multiple transcript variants. [provided by RefSeq, Dec 2014]