L Kynurenine Hydrolase (KYNU) (NM_003937) Human Tagged ORF Clone

CAT#: RG214932

KYNU (tGFP-tagged) - Human kynureninase (KYNU), transcript variant 1

Product Images

Circular map for RG214932

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: L Kynurenine Hydrolase
• Synonyms: KYNUU; VCRL2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG214932 representing NM_003937
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCTTCATCTCTTGAGCTGCCGGCTGACACAGTGCAGCGCATTGCGGCTGAACTCAAATGCCACC
  CAACGGATGAGAGGGTGGCTCTCCACCTAGATGAGGAAGATAAGCTGAGGCACTTCAGGGAGTGCTTTTA
  TATTCCCAAAATACAGGATCTGCCTCCAGTTGATTTATCATTAGTGAATAAAGATGAAAATGCCATCTAT
  TTCTTGGGAAATTCTCTTGGCCTTCAACCAAAAATGGTTAAAACATATCTTGAAGAAGAACTAGATAAGT
  GGGCCAAAATAGCAGCCTATGGTCATGAAGTGGGGAAGCGTCCTTGGATTACAGGAGATGAGAGTATTGT
  AGGCCTTATGAAGGACATTGTAGGAGCCAATGAGAAAGAAATAGCCCTAATGAATGCTTTGACTGTAAAT
  TTACATCTTCTAATGTTATCATTTTTTAAGCCTACGCCAAAACGATATAAAATTCTTCTAGAAGCCAAAG
  CCTTCCCTTCTGATCATTATGCTATTGAGTCACAACTACAACTTCACGGACTTAACATTGAAGAAAGTAT
  GCGGATGATAAAGCCAAGAGAGGGGGAAGAAACCTTAAGAATAGAGGATATCCTTGAAGTAATTGAGAAG
  GAAGGAGACTCAATTGCAGTGATCCTGTTCAGTGGGGTGCATTTTTACACTGGACAGCACTTTAATATTC
  CTGCCATCACAAAAGCTGGACAAGCGAAGGGTTGTTATGTTGGCTTTGATCTAGCACATGCAGTTGGAAA
  TGTTGAACTCTACTTACATGACTGGGGAGTTGATTTTGCCTGCTGGTGTTCCTACAAGTATTTAAATGCA
  GGAGCAGGAGGAATTGCTGGTGCCTTCATTCATGAAAAGCATGCCCATACGATTAAACCTGCATTAGTGG
  GATGGTTTGGCCATGAACTCAGCACCAGATTTAAGATGGATAACAAACTGCAGTTAATCCCTGGGGTCTG
  TGGATTCCGAATTTCAAATCCTCCCATTTTGTTGGTCTGTTCCTTGCATGCTAGTTTAGAGATCTTTAAG
  CAAGCGACAATGAAGGCATTGCGGAAAAAATCTGTTTTGCTAACTGGCTATCTGGAATACCTGATCAAGC
  ATAACTATGGCAAAGATAAAGCAGCAACCAAGAAACCAGTTGTGAACATAATTACTCCGTCTCATGTAGA
  GGAGCGGGGGTGCCAGCTAACAATAACATTTTCTGTTCCAAACAAAGATGTTTTCCAAGAACTAGAAAAA
  AGAGGAGTGGTTTGTGACAAGCGGAATCCAAATGGCATTCGAGTGGCTCCAGTTCCTCTCTATAATTCTT
  TCCATGATGTTTATAAATTTACCAATCTGCTCACTTCTATACTTGACTCTGCAGAAACAAAAAAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG214932 representing NM_003937
  Red=Cloning site Green=Tags(s)
  
  MEPSSLELPADTVQRIAAELKCHPTDERVALHLDEEDKLRHFRECFYIPKIQDLPPVDLSLVNKDENAIY
  FLGNSLGLQPKMVKTYLEEELDKWAKIAAYGHEVGKRPWITGDESIVGLMKDIVGANEKEIALMNALTVN
  LHLLMLSFFKPTPKRYKILLEAKAFPSDHYAIESQLQLHGLNIEESMRMIKPREGEETLRIEDILEVIEK
  EGDSIAVILFSGVHFYTGQHFNIPAITKAGQAKGCYVGFDLAHAVGNVELYLHDWGVDFACWCSYKYLNA
  GAGGIAGAFIHEKHAHTIKPALVGWFGHELSTRFKMDNKLQLIPGVCGFRISNPPILLVCSLHASLEIFK
  QATMKALRKKSVLLTGYLEYLIKHNYGKDKAATKKPVVNIITPSHVEERGCQLTITFSVPNKDVFQELEK
  RGVVCDKRNPNGIRVAPVPLYNSFHDVYKFTNLLTSILDSAETKN
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003937
• ORF Size: 1395 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003937.3
• RefSeq Size: 1688 bp
• RefSeq ORF: 1398 bp
• Locus ID: 8942
• UniProt ID: Q16719
• Cytogenetics: 2q22.2
• Protein Families: Protease
• Protein Pathways: Metabolic pathways, Tryptophan metabolism
• Gene Summary: Kynureninase is a pyridoxal-5'-phosphate (pyridoxal-P) dependent enzyme that catalyzes the cleavage of L-kynurenine and L-3-hydroxykynurenine into anthranilic and 3-hydroxyanthranilic acids, respectively. Kynureninase is involved in the biosynthesis of NAD cofactors from tryptophan through the kynurenine pathway. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2010]