EGFR (NM_201283) Human Tagged ORF Clone
EGFR (GFP-tagged) - Human epidermal growth factor receptor (EGFR), transcript variant 3
|Product Name||EGFR (NM_201283) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|Synonyms||ERBB; ERBB1; HER1; mENA; NISBD2; PIG61|
|E. coli Selection||Ampicillin (100 ug/mL)|
>RG214877 representing NM_201283
Red=Cloning site Blue=ORF Green=Tags(s)
ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG214877 representing NM_201283
Red=Cloning site Green=Tags(s)
TRTRPLE - GFP Tag - V
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||1215 bp|
|OTI Disclaimer||Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at firstname.lastname@example.org or by calling 301.340.3188 option 3 for pricing and delivery.
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||1595 bp|
|RefSeq ORF||1218 bp|
|Protein Families||Adult stem cells, Cancer stem cells, Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase, Secreted Protein, Stem cell relevant signaling - JAK/STAT signaling pathway, Transmembrane|
|Protein Pathways||Adherens junction, Bladder cancer, Calcium signaling pathway, Colorectal cancer, Cytokine-cytokine receptor interaction, Dorso-ventral axis formation, Endocytosis, Endometrial cancer, Epithelial cell signaling in Helicobacter pylori infection, ErbB signaling pathway, Focal adhesion, Gap junction, Glioma, GnRH signaling pathway, MAPK signaling pathway, Melanoma, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Prostate cancer, Regulation of actin cytoskeleton|
|Gene Summary||The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq, Jun 2016]|
|cDNA Clone Resources|
|SC308010||EGFR (untagged)-Human epidermal growth factor receptor (EGFR), transcript variant 3||
|RC214877||EGFR (Myc-DDK-tagged)-Human epidermal growth factor receptor (EGFR), transcript variant 3||
|RC600003||EGFR (DDK-His-tagged)-Extra Cellular Domain Clone of Homo sapiens epidermal growth factor receptor, transcript variant 3, Signal peptide (1-24) plus EC domain (25-405)||
|RC214877L1||Lenti ORF clone of Human epidermal growth factor receptor (EGFR), transcript variant 3, Myc-DDK-tagged||
|RC214877L2||Lenti ORF clone of Human epidermal growth factor receptor (EGFR), transcript variant 3, mGFP tagged||
|RC214877L3||Lenti ORF clone of Human epidermal growth factor receptor (EGFR), transcript variant 3, Myc-DDK-tagged||
|RC214877L4||Lenti ORF clone of Human epidermal growth factor receptor (EGFR), transcript variant 3, mGFP tagged||