ARID1B (NM_020732) Human Tagged ORF Clone

CAT#: RG214830

ARID1B (tGFP-tagged) - Human AT rich interactive domain 1B (SWI1-like) (ARID1B), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ARID1B
• Synonyms: 6A3-5; BAF250B; BRIGHT; CSS1; DAN15; ELD/OSA1; MRD12; OSA2; P250R
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG214830 representing NM_020732
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGACGGGGCTGCTCCCCAACCACAAACTGAAAACCGTTGGCGAAGCCCCCGCCGCGCCGCCCCACC
  AGCAGCACCACCACCACCACCATGCCCACCACCACCACCACCATGCCCACCACCTCCACCACCACCACGC
  ACTACAGCAGCAGCTAAACCAGTTCCAGCAGCAGCAGCAGCAGCAGCAACAGCAGCAGCAGCAGCAGCAG
  CAACAGCAACATCCCATTTCCAACAACAACAGCTTGGGCGGCGCGGGCGGCGGCGCGCCTCAGCCCGGCC
  CCGACATGGAGCAGCCGCAACATGGAGGCGCCAAGGACAGTGCTGCGGGCGGCCAGGCCGACCCCCCGGG
  CCCGCCGCTGCTGAGCAAGCCGGGCGACGAGGACGACGCGCCGCCCAAGATGGGGGAGCCGGCGGGCGGC
  CGCTACGAGCACCCGGGCTTGGGCGCCCTGGGCACGCAGCAGCCGCCGGTCGCCGTGCCCGGGGGCGGCG
  GCGGCCCGGCGGCCGTCCCGGAGTTTAATAATTACTATGGCAGCGCTGCCCCTGCGAGCGGCGGCCCCGG
  CGGCCGCGCTGGGCCTTGCTTTGATCAACATGGCGGACAACAAAGCCCCGGGATGGGGATGATGCACTCC
  GCCTCCGCCGCCGCCGCCGGGGCCCCCGGCAGCATGGACCCCCTGCAGAACTCCCACGAAGGGTACCCCA
  ACAGCCAGTGCAACCATTATCCGGGCTACAGCCGGCCCGGCGCGGGCGGCGGCGGCGGCGGCGGCGGCGG
  AGGAGGAGGAGGCAGCGGAGGAGGAGGAGGAGGAGGAGGAGCAGGAGCAGGAGGAGCAGGAGCGGGAGCT
  GTGGCGGCGGCGGCCGCGGCGGCGGCGGCAGCAGCAGGAGGCGGCGGCGGCGGCGGCTATGGGGGCTCGT
  CCGCGGGGTACGGGGTGCTGAGCTCCCCCCGGCAGCAGGGCGGCGGCATGATGATGGGCCCCGGGGGCGG
  CGGGGCCGCGAGCCTCAGCAAGGCGGCCGCCGGCTCGGCGGCGGGGGGCTTCCAGCGCTTCGCCGGCCAG
  AACCAGCACCCGTCGGGGGCCACCCCGACCCTCAATCAGCTGCTCACCTCGCCCAGCCCCATGATGCGGA
  GCTACGGCGGCAGCTACCCCGAGTACAGCAGCCCCAGCGCGCCGCCGCCGCCGCCGTCGCAGCCCCAGTC
  CCAGGCGGCGGCGGCGGGGGCGGCGGCGGGCGGCCAGCAGGCGGCCGCGGGCATGGGCTTGGGCAAGGAC
  ATGGGCGCCCAGTACGCCGCTGCCAGCCCGGCCTGGGCGGCCGCGCAACAAAGGAGTCACCCGGCGATGA
  GCCCCGGCACCCCCGGACCGACCATGGGCAGATCCCAGGGCAGCCCAATGGATCCAATGGTGATGAAGAG
  ACCTCAGTTGTATGGCATGGGCAGTAACCCTCATTCTCAGCCTCAGCAGAGCAGTCCGTACCCAGGAGGT
  TCCTATGGCCCTCCAGGCCCACAGCGGTATCCAATTGGCATCCAGGGTCGGACTCCCGGGGCCATGGCCG
  GAATGCAGTACCCTCAGCAGCAGGACTCTGGAGATGCCACATGGAAAGAAACATTCTGGTTGATGCCACC
  TCAGTATGGACAGCAAGGTGTGAGTGGTTACTGCCAGCAGGGCCAACAGCCATATTACAGCCAGCAGCCG
  CAGCCCCCGCACCTCCCACCCCAGGCGCAGTATCTGCCGTCCCAGTCCCAGCAGAGGTACCAGCCGCAGC
  AGGACATGTCTCAGGAAGGCTATGGAACTAGATCTCAACCTCCTCTGGCCCCCGGAAAACCTAACCATGA
  AGACTTGAACTTAATACAGCAAGAAAGACCATCAAGTTTACCAGATCTGTCTGGCTCCATTGATGACCTC
  CCCACGGGAACGGAAGCAACTTTGAGCTCAGCAGTCAGTGCATCCGGGTCCACGAGCAGCCAAGGGGATC
  AGAGCAACCCGGCGCAGTCGCCTTTCTCCCCACATGCGTCCCCTCATCTCTCCAGCATCCCGGGGGGCCC
  ATCTCCCTCTCCTGTTGGCTCTCCTGTAGGAAGCAACCAGTCTCGATCTGGCCCAATCTCTCCTGCAAGT
  ATCCCAGGTAGTCAGATGCCTCCGCAGCCACCCGGGAGCCAGTCAGAATCCAGTTCCCATCCCGCCTTGA
  GCCAGTCACCAATGCCACAGGAAAGAGGTTTTATGGCAGGCACACAAAGAAACCCTCAGATGGCTCAGTA
  TGGACCTCAACAGACAGGACCATCCATGTCGCCTCATCCTTCTCCTGGGGGCCAGATGCATGCTGGAATC
  AGTAGCTTTCAGCAGAGTAACTCAAGTGGGACTTACGGTCCACAGATGAGCCAGTATGGACCACAAGGTA
  ACTACTCCAGACCCCCAGCGTATAGTGGGGTGCCCAGTGCAAGCTACAGCGGCCCAGGGCCCGGTATGGG
  TATCAGTGCCAACAACCAGATGCATGGACAAGGGCCAAGCCAGCCATGTGGTGCTGTGCCCCTGGGACGA
  ATGCCATCAGCTGGGATGCAGAACAGACCATTTCCTGGAAATATGAGCAGCATGACCCCCAGTTCTCCTG
  GCATGTCTCAGCAGGGAGGGCCAGGAATGGGGCCGCCAATGCCAACTGTGAACCGTAAGGCACAGGAGGC
  AGCCGCAGCAGTGATGCAGGCTGCTGCGAACTCAGCACAAAGCAGGCAAGGCAGTTTCCCCGGCATGAAC
  CAGAGTGGACTTATGGCTTCCAGCTCTCCCTACAGCCAGCCCATGAACAACAGCTCTAGCCTGATGAACA
  CGCAGGCGCCGCCCTACAGCATGGCGCCCGCCATGGTGAACAGCTCGGCAGCATCTGTGGGTCTTGCAGA
  TATGATGTCTCCTGGTGAATCCAAACTGCCCCTGCCTCTCAAAGCAGACGGCAAAGAAGAAGGCACTCCA
  CAGCCCGAGAGCAAGTCAAAGAAGTCCAGCTCCTCCACCACTACTGGGGAGAAGATCACGAAGGTGTACG
  AGCTGGGGAATGAGCCAGAGAGAAAGCTCTGGGTCGACCGATACCTCACCTTCATGGAAGAGAGAGGCTC
  TCCTGTCTCAAGTCTGCCTGCCGTGGGCAAGAAGCCCCTGGACCTGTTCCGACTCTACGTCTGCGTCAAA
  GAGATCGGGGGTTTGGCCCAGGTTAATAAAAACAAGAAGTGGCGTGAGCTGGCAACCAACCTAAACGTTG
  GCACCTCAAGCAGTGCAGCGAGCTCCCTGAAAAAGCAGTATATTCAGTACCTGTTTGCCTTTGAGTGCAA
  GATCGAACGTGGGGAGGAGCCCCCGCCGGAAGTCTTCAGCACCGGGGACACCAAAAAGCAGCCCAAGCTC
  CAGCCGCCATCTCCTGCTAACTCGGGATCCTTGCAAGGCCCACAGACCCCCCAGTCAACTGGCAGCAATT
  CCATGGCAGAGGTTCCAGGTGACCTGAAGCCACCTACCCCAGCCTCCACCCCTCACGGCCAGATGACTCC
  AATGCAAGGTGGAAGAAGCAGTACAATCAGTGTGCACGACCCATTCTCAGATGTGAGTGATTCATCCTTC
  CCGAAACGGAACTCCATGACTCCAAACGCCCCCTACCAGCAGGGCATGAGCATGCCCGATGTGATGGGCA
  GGATGCCCTATGAGCCCAACAAGGACCCCTTTGGGGGAATGAGAAAAGTGCCTGGAAGCAGCGAGCCCTT
  TATGACGCAAGGACAGATGCCCAACAGCAGCATGCAGGACATGTACAACCAAAGTCCCTCCGGAGCAATG
  TCTAACCTGGGCATGGGGCAGCGCCAGCAGTTTCCCTATGGAGCCAGTTACGACCGAAGGCATGAACCTT
  ATGGGCAGCAGTATCCAGGCCAAGGCCCTCCCTCGGGACAGCCGCCGTATGGAGGGCACCAGCCCGGCCT
  GTACCCACAGCAGCCGAATTACAAACGCCATATGGACGGCATGTACGGGCCCCCAGCCAAGCGCCACGAG
  GGCGACATGTACAACATGCAGTACAGCAGCCAGCAGCAGGAGATGTACAACCAGTATGGAGGCTCCTACT
  CGGGCCCGGACCGCAGGCCCATCCAGGGCCAGTACCCGTATCCCTACAGCAGGGAGAGGATGCAGGGCCC
  GGGGCAGATCCAGACACACGGAATCCCGCCTCAGATGATGGGCGGCCCGCTGCAGTCGTCCTCCAGTGAG
  GGGCCTCAGCAGAATATGTGGGCAGCACGCAATGATATGCCTTATCCCTACCAGAACAGGCAGGGCCCTG
  GCGGCCCTACACAGGCGCCCCCTTACCCAGGCATGAACCGCACAGACGATATGATGGTACCCGATCAGAG
  GATAAATCATGAGAGCCAGTGGCCTTCTCACGTCAGCCAGCGTCAGCCTTATATGTCGTCCTCAGCCTCC
  ATGCAGCCCATCACACGCCCACCACAGCCGTCCTACCAGACGCCACCGTCACTGCCAAATCACATCTCCA
  GGGCGCCCAGCCCAGCGTCCTTCCAGCGCTCCCTGGAGAACCGCATGTCTCCAAGCAAGTCTCCTTTTCT
  GCCGTCTATGAAGATGCAGAAGGTCATGCCCACGGTCCCCACATCCCAGGTCACCGGGCCACCACCCCAA
  CCACCCCCAATCAGAAGGGAGATCACCTTTCCTCCTGGCTCAGTAGAAGCATCACAACCAGTCTTGAAAC
  AAAGGCGAAAGATTACCTCCAAAGATATCGTTACTCCTGAGGCGTGGCGTGTGATGATGTCCCTTAAATC
  AGGTCTTTTGGCTGAGAGTACGTGGGCTTTGGACACTATTAATATTCTTCTGTATGATGACAGCACTGTT
  GCTACTTTCAATCTCTCCCAGTTGTCTGGATTTCTCGAACTTTTAGTCGAGTACTTTAGAAAATGCCTGA
  TTGACATTTTTGGAATTCTTATGGAATATGAAGTGGGAGACCCCAGCCAAAAAGCACTTGATCACAACGC
  AGCAAGGAAGGATGACAGCCAGTCCTTGGCAGACGATTCTGGGAAAGAGGAGGAAGATGCTGAATGTATT
  GATGACGACGAGGAAGACGAGGAGGATGAGGAGGAAGACAGCGAGAAGACAGAAAGCGATGAAAAGAGCA
  GCATCGCTCTGACTGCCCCGGACGCCGCTGCAGACCCAAAGGAGAAGCCCAAGCAAGCCAGTAAGTTCGA
  CAAGCTGCCAATAAAGATAGTCAAAAAGAACAACCTGTTTGTTGTTGACCGATCTGACAAGTTGGGGCGT
  GTGCAGGAGTTCAATAGTGGCCTTCTGCACTGGCAGCTCGGCGGGGGTGACACCACCGAGCACATTCAGA
  CTCACTTTGAGAGCAAGATGGAAATTCCTCCTCGCAGGCGCCCACCTCCCCCCTTAAGCTCCGCAGGTAG
  AAAGAAAGAGCAAGAAGGCAAAGGCGACTCTGAAGAGCAGCAAGAGAAAAGCATCATAGCAACCATCGAT
  GACGTCCTCTCTGCTCGGCCAGGGGCATTGCCTGAAGACGCAAACCCTGGGCCCCAGACCGAAAGCAGTA
  AGTTTCCCTTTGGTATCCAGCAAGCCAAAAGTCACCGGAACATCAAGCTGCTGGAGGACGAGCCCAGGAG
  CCGAGACGAGACTCCTCTGTGTACCATCGCGCACTGGCAGGACTCGCTGGCTAAGCGATGCATCTGTGTG
  TCCAATATTGTCCGTAGCTTGTCATTCGTGCCTGGCAATGATGCCGAAATGTCCAAACATCCAGGCCTGG
  TGCTGATCCTGGGGAAGCTGATTCTTCTTCACCACGAGCATCCAGAGAGAAAGCGAGCACCGCAGACCTA
  TGAGAAAGAGGAGGATGAGGACAAGGGGGTGGCCTGCAGCAAAGATGAGTGGTGGTGGGACTGCCTCGAG
  GTCTTGAGGGATAACACGTTGGTCACGTTGGCCAACATTTCCGGGCAGCTAGACTTGTCTGCTTACACGG
  AAAGCATCTGCTTGCCAATTTTGGATGGCTTGCTGCACTGGATGGTGTGCCCGTCTGCAGAGGCACAAGA
  TCCCTTTCCAACTGTGGGACCCAACTCGGTCCTGTCGCCTCAGAGACTTGTGCTGGAGACCCTCTGTAAA
  CTCAGTATCCAGGACAATAATGTGGACCTGATCTTGGCCACTCCTCCATTTAGTCGTCAGGAGAAATTCT
  ATGCTACATTAGTTAGGTACGTTGGGGATCGCAAAAACCCAGTCTGTCGAGAAATGTCCATGGCGCTTTT
  ATCGAACCTTGCCCAAGGGGACGCACTAGCAGCAAGGGCCATAGCTGTGCAGAAAGGAAGCATTGGAAAC
  TTGATAAGCTTCCTAGAGGATGGGGTCACGATGGCCCAGTACCAGCAGAGCCAGCACAACCTCATGCACA
  TGCAGCCCCCGCCCCTGGAACCACCTAGCGTAGACATGATGTGCAGGGCGGCCAAGGCTTTGCTAGCCAT
  GGCCAGAGTGGACGAAAACCGCTCGGAATTCCTTTTGCACGAGGGCCGGTTGCTGGATATCTCGATATCA
  GCTGTCCTGAACTCTCTGGTTGCATCTGTCATCTGTGATGTACTGTTTCAGATTGGGCAGTTA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG214830 representing NM_020732
  Red=Cloning site Green=Tags(s)
  
  METGLLPNHKLKTVGEAPAAPPHQQHHHHHHAHHHHHHAHHLHHHHALQQQLNQFQQQQQQQQQQQQQQQ
  QQQHPISNNNSLGGAGGGAPQPGPDMEQPQHGGAKDSAAGGQADPPGPPLLSKPGDEDDAPPKMGEPAGG
  RYEHPGLGALGTQQPPVAVPGGGGGPAAVPEFNNYYGSAAPASGGPGGRAGPCFDQHGGQQSPGMGMMHS
  ASAAAAGAPGSMDPLQNSHEGYPNSQCNHYPGYSRPGAGGGGGGGGGGGGGSGGGGGGGGAGAGGAGAGA
  VAAAAAAAAAAAGGGGGGGYGGSSAGYGVLSSPRQQGGGMMMGPGGGGAASLSKAAAGSAAGGFQRFAGQ
  NQHPSGATPTLNQLLTSPSPMMRSYGGSYPEYSSPSAPPPPPSQPQSQAAAAGAAAGGQQAAAGMGLGKD
  MGAQYAAASPAWAAAQQRSHPAMSPGTPGPTMGRSQGSPMDPMVMKRPQLYGMGSNPHSQPQQSSPYPGG
  SYGPPGPQRYPIGIQGRTPGAMAGMQYPQQQDSGDATWKETFWLMPPQYGQQGVSGYCQQGQQPYYSQQP
  QPPHLPPQAQYLPSQSQQRYQPQQDMSQEGYGTRSQPPLAPGKPNHEDLNLIQQERPSSLPDLSGSIDDL
  PTGTEATLSSAVSASGSTSSQGDQSNPAQSPFSPHASPHLSSIPGGPSPSPVGSPVGSNQSRSGPISPAS
  IPGSQMPPQPPGSQSESSSHPALSQSPMPQERGFMAGTQRNPQMAQYGPQQTGPSMSPHPSPGGQMHAGI
  SSFQQSNSSGTYGPQMSQYGPQGNYSRPPAYSGVPSASYSGPGPGMGISANNQMHGQGPSQPCGAVPLGR
  MPSAGMQNRPFPGNMSSMTPSSPGMSQQGGPGMGPPMPTVNRKAQEAAAAVMQAAANSAQSRQGSFPGMN
  QSGLMASSSPYSQPMNNSSSLMNTQAPPYSMAPAMVNSSAASVGLADMMSPGESKLPLPLKADGKEEGTP
  QPESKSKKSSSSTTTGEKITKVYELGNEPERKLWVDRYLTFMEERGSPVSSLPAVGKKPLDLFRLYVCVK
  EIGGLAQVNKNKKWRELATNLNVGTSSSAASSLKKQYIQYLFAFECKIERGEEPPPEVFSTGDTKKQPKL
  QPPSPANSGSLQGPQTPQSTGSNSMAEVPGDLKPPTPASTPHGQMTPMQGGRSSTISVHDPFSDVSDSSF
  PKRNSMTPNAPYQQGMSMPDVMGRMPYEPNKDPFGGMRKVPGSSEPFMTQGQMPNSSMQDMYNQSPSGAM
  SNLGMGQRQQFPYGASYDRRHEPYGQQYPGQGPPSGQPPYGGHQPGLYPQQPNYKRHMDGMYGPPAKRHE
  GDMYNMQYSSQQQEMYNQYGGSYSGPDRRPIQGQYPYPYSRERMQGPGQIQTHGIPPQMMGGPLQSSSSE
  GPQQNMWAARNDMPYPYQNRQGPGGPTQAPPYPGMNRTDDMMVPDQRINHESQWPSHVSQRQPYMSSSAS
  MQPITRPPQPSYQTPPSLPNHISRAPSPASFQRSLENRMSPSKSPFLPSMKMQKVMPTVPTSQVTGPPPQ
  PPPIRREITFPPGSVEASQPVLKQRRKITSKDIVTPEAWRVMMSLKSGLLAESTWALDTINILLYDDSTV
  ATFNLSQLSGFLELLVEYFRKCLIDIFGILMEYEVGDPSQKALDHNAARKDDSQSLADDSGKEEEDAECI
  DDDEEDEEDEEEDSEKTESDEKSSIALTAPDAAADPKEKPKQASKFDKLPIKIVKKNNLFVVDRSDKLGR
  VQEFNSGLLHWQLGGGDTTEHIQTHFESKMEIPPRRRPPPPLSSAGRKKEQEGKGDSEEQQEKSIIATID
  DVLSARPGALPEDANPGPQTESSKFPFGIQQAKSHRNIKLLEDEPRSRDETPLCTIAHWQDSLAKRCICV
  SNIVRSLSFVPGNDAEMSKHPGLVLILGKLILLHHEHPERKRAPQTYEKEEDEDKGVACSKDEWWWDCLE
  VLRDNTLVTLANISGQLDLSAYTESICLPILDGLLHWMVCPSAEAQDPFPTVGPNSVLSPQRLVLETLCK
  LSIQDNNVDLILATPPFSRQEKFYATLVRYVGDRKNPVCREMSMALLSNLAQGDALAARAIAVQKGSIGN
  LISFLEDGVTMAQYQQSQHNLMHMQPPPLEPPSVDMMCRAAKALLAMARVDENRSEFLLHEGRLLDISIS
  AVLNSLVASVICDVLFQIGQL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020732
• ORF Size: 6573 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020732.2, NP_065783.2
• RefSeq Size: 8126 bp
• RefSeq ORF: 6750 bp
• Locus ID: 57492
• UniProt ID: Q8NFD5
• Cytogenetics: 6q25.3
• Domains: ARID
• Gene Summary: This locus encodes an AT-rich DNA interacting domain-containing protein. The encoded protein is a component of the SWI/SNF chromatin remodeling complex and may play a role in cell-cycle activation. The protein encoded by this locus is similar to AT-rich interactive domain-containing protein 1A. These two proteins function as alternative, mutually exclusive ARID-subunits of the SWI/SNF complex. The associated complexes play opposing roles. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2016]