CRMP3 (DPYSL4) (NM_006426) Human Tagged ORF Clone

CAT#: RG214821

DPYSL4 (tGFP-tagged) - Human dihydropyrimidinase-like 4 (DPYSL4)

Product Images

Circular map for RG214821

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CRMP3
• Synonyms: CRMP3; DRP-4; ULIP4
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG214821 representing NM_006426
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCTTCCAGGGCAAGAAAAGCATCCCCCGGATCACGAGTGACCGCCTTCTGATCAGAGGTGGGAGGA
  TCGTGAATGACGACCAGTCCTTTTACGCTGATGTGCACGTGGAAGATGGCTTGATAAAACAAATCGGAGA
  AAACCTCATCGTCCCTGGGGGCATCAAGACCATTGACGCCCACGGCCTGATGGTCCTTCCTGGTGGCGTT
  GACGTCCACACAAGGCTGCAGATGCCTGTCCTGGGCATGACACCGGCTGACGACTTCTGTCAGGGCACCA
  AGGCAGCGCTAGCAGGAGGAACCACCATGATCTTGGACCACGTCTTCCCCGACACGGGTGTGAGCCTGCT
  GGCGGCCTACGAGCGGTGGCGGGAGCGGGCGGACAGCGCGGCCTGCTGCGACTACTCCCTGCACGTGGAC
  ATCACCCGATGGCATGAGAGCATCAAGGAGGAGCTGGAGGCCCTGGTCAAGGAGAAGGGTGTGAACTCCT
  TCCTGGTCTTCATGGCATACAAGGACCGGTGCCAGTGCAGCGACAGCCAGATGTACGAGATCTTCAGCAT
  CATCCGGGACCTGGGGGCCTTGGCCCAGGTGCACGCTGAGAACGGGGACATCGTGGAGGAGGAGCAGAAG
  CGGTTGCTGGAGCTCGGCATCACTGGCCCCGAGGGCCACGTGCTCAGCCACCCCGAGGAGGTGGAGGCTG
  AGGCGGTGTACCGAGCTGTCACCATCGCCAAGCAGGCAAACTGCCCGCTGTACGTCACCAAGGTGATGAG
  CAAGGGGGCGGCCGACGCCATCGCTCAGGCCAAGCGCAGAGGGGTGGTCGTGTTTGGGGAGCCCATCACC
  GCCAGCCTGGGCACCGACGGTTCACACTACTGGAGCAAGAACTGGGCCAAGGCCGCAGCCTTCGTCACAT
  CACCCCCTGTCAACCCAGACCCCACCACGGCAGACCACCTCACCTGCTTGCTGTCCAGCGGGGACCTCCA
  GGTGACAGGCAGCGCCCACTGCACCTTCACCACTGCCCAGAAGGCTGTGGGCAAGGACAACTTCGCGCTG
  ATCCCCGAGGGCACCAACGGCATTGAGGAGCGCATGTCGATGGTCTGGGAGAAATGTGTGGCCTCTGGGA
  AGATGGACGAGAATGAGTTCGTCGCGGTGACCAGTACAAATGCTGCCAAAATCTTCAATTTTTACCCAAG
  GAAGGGGCGAGTGGCTGTGGGCTCTGACGCTGACCTGGTCATATGGAACCCCAAGGCCACCAAGATCATC
  TCTGCCAAGACCCACAATCTGAACGTGGAGTACAACATCTTCGAGGGAGTGGAGTGCCGGGGAGCGCCTG
  CCGTGGTCATAAGTCAGGGCCGAGTGGCGCTGGAGGACGGGAAGATGTTTGTCACCCCGGGGGCGGGCCG
  CTTCGTCCCTCGGAAAACATTCCCGGACTTTGTCTACAAGAGGATCAAAGCTCGCAACAGGCTGGCGGAG
  ATCCACGGTGTGCCCCGTGGGCTGTATGACGGGCCCGTCCACGAGGTGATGGTGCCTGCCAAGCCAGGGA
  GTGGCGCTCCGGCCCGCGCGTCCTGCCCAGGCAAGATCTCCGTGCCTCCTGTGCGCAACCTACATCAGTC
  GGGGTTCAGCCTATCTGGGTCTCAGGCTGATGACCACATCGCCCGACGCACAGCACAGAAGATCATGGCA
  CCACCTGGCGGCCGCTCCAACATCACCTCTCTCTCC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG214821 representing NM_006426
  Red=Cloning site Green=Tags(s)
  
  MSFQGKKSIPRITSDRLLIRGGRIVNDDQSFYADVHVEDGLIKQIGENLIVPGGIKTIDAHGLMVLPGGV
  DVHTRLQMPVLGMTPADDFCQGTKAALAGGTTMILDHVFPDTGVSLLAAYERWRERADSAACCDYSLHVD
  ITRWHESIKEELEALVKEKGVNSFLVFMAYKDRCQCSDSQMYEIFSIIRDLGALAQVHAENGDIVEEEQK
  RLLELGITGPEGHVLSHPEEVEAEAVYRAVTIAKQANCPLYVTKVMSKGAADAIAQAKRRGVVVFGEPIT
  ASLGTDGSHYWSKNWAKAAAFVTSPPVNPDPTTADHLTCLLSSGDLQVTGSAHCTFTTAQKAVGKDNFAL
  IPEGTNGIEERMSMVWEKCVASGKMDENEFVAVTSTNAAKIFNFYPRKGRVAVGSDADLVIWNPKATKII
  SAKTHNLNVEYNIFEGVECRGAPAVVISQGRVALEDGKMFVTPGAGRFVPRKTFPDFVYKRIKARNRLAE
  IHGVPRGLYDGPVHEVMVPAKPGSGAPARASCPGKISVPPVRNLHQSGFSLSGSQADDHIARRTAQKIMA
  PPGGRSNITSLS
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006426
• ORF Size: 1716 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006426.1, NP_006417.1
• RefSeq Size: 2699 bp
• RefSeq ORF: 1719 bp
• Locus ID: 10570
• UniProt ID: O14531
• Cytogenetics: 10q26.3
• Domains: Amidohydro_1
• Gene Summary: Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration (By similarity).[UniProtKB/Swiss-Prot Function]