Metabotropic Glutamate Receptor 1 (GRM1) (NM_000838) Human Tagged ORF Clone

CAT#: RG214400

GRM1 (tGFP-tagged) - Human glutamate receptor, metabotropic 1 (GRM1), transcript variant 1

Product Images

Circular map for RG214400

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Metabotropic Glutamate Receptor 1
• Synonyms: GPRC1A; GRM1A; mGlu1; MGLUR1; MGLUR1A; SCAR13
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG214400 representing NM_000838
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCGGGCTCCTTTTGTTTTTTTTCCCAGCGATCTTTTTGGAGGTGTCCCTTCTCCCCAGAAGCCCCG
  GCAGGAAAGTGTTGCTGGCAGGAGCGTCGTCTCAGCGCTCGGTGGCCAGAATGGACGGAGATGTCATCAT
  TGGAGCCCTCTTCTCAGTCCATCACCAGCCTCCGGCCGAGAAAGTGCCCGAGAGGAAGTGTGGGGAGATC
  AGGGAGCAGTATGGCATCCAGAGGGTGGAGGCCATGTTCCACACGTTGGATAAGATCAACGCGGACCCGG
  TCCTCCTGCCCAACATCACCCTGGGCAGTGAGATCCGGGACTCCTGCTGGCACTCTTCCGTGGCTCTGGA
  ACAGAGCATTGAGTTCATTAGGGACTCTCTGATTTCCATTCGAGATGAGAAGGATGGGATCAACCGGTGT
  CTGCCTGACGGCCAGTCCCTCCCCCCAGGCAGGACTAAGAAGCCCATTGCGGGAGTGATCGGTCCCGGCT
  CCAGCTCTGTAGCCATTCAAGTGCAGAACCTGCTCCAGCTCTTCGACATCCCCCAGATCGCTTATTCAGC
  CACAAGCATCGACCTGAGTGACAAAACTTTGTACAAATACTTCCTGAGGGTTGTCCCTTCTGACACTTTG
  CAGGCAAGGGCCATGCTTGACATAGTCAAACGTTACAATTGGACCTATGTCTCTGCAGTCCACACGGAAG
  GGAATTATGGGGAGAGCGGAATGGACGCTTTCAAAGAGCTGGCTGCCCAGGAAGGCCTCTGTATCGCCCA
  TTCTGACAAAATCTACAGCAACGCTGGGGAGAAGAGCTTTGACCGACTCTTGCGCAAACTCCGAGAGAGG
  CTTCCCAAGGCTAGAGTGGTGGTCTGCTTCTGTGAAGGCATGACAGTGCGAGGACTCCTGAGCGCCATGC
  GGCGCCTTGGCGTCGTGGGCGAGTTCTCACTCATTGGAAGTGATGGATGGGCAGACAGAGATGAAGTCAT
  TGAAGGTTATGAGGTGGAAGCCAACGGGGGAATCACGATAAAGCTGCAGTCTCCAGAGGTCAGGTCATTT
  GATGATTATTTCCTGAAACTGAGGCTGGACACTAACACGAGGAATCCCTGGTTCCCTGAGTTCTGGCAAC
  ATCGGTTCCAGTGCCGCCTTCCAGGACACCTTCTGGAAAATCCCAACTTTAAACGAATCTGCACAGGCAA
  TGAAAGCTTAGAAGAAAACTATGTCCAGGACAGTAAGATGGGGTTTGTCATCAATGCCATCTATGCCATG
  GCACATGGGCTGCAGAACATGCACCATGCCCTCTGCCCTGGCCACGTGGGCCTCTGCGATGCCATGAAGC
  CCATCGACGGCAGCAAGCTGCTGGACTTCCTCATCAAGTCCTCATTCATTGGAGTATCTGGAGAGGAGGT
  GTGGTTTGATGAGAAAGGAGACGCTCCTGGAAGGTATGATATCATGAATCTGCAGTACACTGAAGCTAAT
  CGCTATGACTATGTGCACGTTGGAACCTGGCATGAAGGAGTGCTGAACATTGATGATTACAAAATCCAGA
  TGAACAAGAGTGGAGTGGTGCGGTCTGTGTGCAGTGAGCCTTGCTTAAAGGGCCAGATTAAGGTTATACG
  GAAAGGAGAAGTGAGCTGCTGCTGGATTTGCACGGCCTGCAAAGAGAATGAATATGTGCAAGATGAGTTC
  ACCTGCAAAGCTTGTGACTTGGGATGGTGGCCCAATGCAGATCTAACAGGCTGTGAGCCCATTCCTGTGC
  GCTATCTTGAGTGGAGCAACATCGAATCCATTATAGCCATCGCCTTTTCATGCCTGGGAATCCTTGTTAC
  CTTGTTTGTCACCCTAATCTTTGTACTGTACCGGGACACACCAGTGGTCAAATCCTCCAGTCGGGAGCTC
  TGCTACATCATCCTAGCTGGCATCTTCCTTGGTTATGTGTGCCCATTCACTCTCATTGCCAAACCTACTA
  CCACCTCCTGCTACCTCCAGCGCCTCTTGGTTGGCCTCTCCTCTGCGATGTGCTACTCTGCTTTAGTGAC
  TAAAACCAATCGTATTGCACGCATCCTGGCTGGCAGCAAGAAGAAGATCTGCACCCGGAAGCCCAGGTTC
  ATGAGTGCCTGGGCTCAGGTGATCATTGCCTCAATTCTGATTAGTGTGCAACTAACCCTGGTGGTAACCC
  TGATCATCATGGAACCCCCTATGCCCATTCTGTCCTACCCAAGTATCAAGGAAGTCTACCTTATCTGCAA
  TACCAGCAACCTGGGTGTGGTGGCCCCTTTGGGCTACAATGGACTCCTCATCATGAGCTGTACCTACTAT
  GCCTTCAAGACCCGCAACGTGCCCGCCAACTTCAACGAGGCCAAATATATCGCGTTCACCATGTACACCA
  CCTGTATCATCTGGCTAGCTTTTGTGCCCATTTACTTTGGGAGCAACTACAAGATCATCACAACTTGCTT
  TGCAGTGAGTCTCAGTGTAACAGTGGCTCTGGGGTGCATGTTCACTCCCAAGATGTACATCATTATTGCC
  AAGCCTGAGAGGAATGTCCGCAGTGCCTTCACCACCTCTGATGTTGTCCGCATGCATGTTGGCGATGGCA
  AGCTGCCCTGCCGCTCCAACACTTTCCTCAACATCTTCCGAAGAAAGAAGGCAGGGGCAGGGAATGCCAA
  TTCTAATGGCAAGTCTGTGTCATGGTCTGAACCAGGTGGAGGACAGGTGCCCAAGGGACAGCATATGTGG
  CACCGCCTCTCTGTGCACGTGAAGACCAATGAGACGGCCTGCAACCAAACAGCCGTCATCAAGCCCCTCA
  CTAAAAGTTACCAAGGCTCTGGCAAGAGCCTGACCTTTTCAGATACCAGCACCAAGACCCTTTACAACGT
  AGAGGAGGAGGAGGATGCCCAGCCGATTCGCTTTAGCCCGCCTGGTAGCCCTTCCATGGTGGTGCACAGG
  CGCGTGCCAAGCGCGGCGACCACTCCGCCTCTGCCGTCCCACCTGACCGCAGAGGAGACCCCCCTCTTCC
  TGGCCGAACCAGCCCTCCCCAAGGGCTTGCCCCCTCCTCTCCAGCAGCAGCAGCAACCCCCTCCACAGCA
  GAAATCGCTGATGGACCAGCTCCAGGGAGTGGTCAGCAACTTCAGTACCGCGATCCCGGATTTTCACGCG
  GTGCTGGCAGGCCCCGGGGGTCCCGGGAACGGGCTGCGGTCCCTGTACCCGCCCCCGCCACCTCCGCAGC
  ACCTGCAGATGCTGCCGCTGCAGCTGAGCACCTTTGGGGAGGAGCTGGTCTCCCCGCCCGCGGACGACGA
  CGACGACAGCGAGAGGTTTAAGCTCCTCCAGGAGTACGTGTATGAGCACGAGCGGGAAGGGAACACGGAA
  GAAGACGAACTGGAAGAGGAGGAGGAGGACCTGCAGGCGGCCAGCAAACTGACCCCGGATGATTCGCCTG
  CGCTGACGCCTCCGTCGCCTTTCCGCGACTCGGTGGCCTCGGGCAGCTCGGTGCCCAGCTCCCCCGTGTC
  CGAGTCGGTGCTCTGCACCCCTCCCAACGTATCCTACGCCTCTGTCATTCTGCGGGACTACAAGCAAAGC
  TCTTCCACCCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG214400 representing NM_000838
  Red=Cloning site Green=Tags(s)
  
  MVGLLLFFFPAIFLEVSLLPRSPGRKVLLAGASSQRSVARMDGDVIIGALFSVHHQPPAEKVPERKCGEI
  REQYGIQRVEAMFHTLDKINADPVLLPNITLGSEIRDSCWHSSVALEQSIEFIRDSLISIRDEKDGINRC
  LPDGQSLPPGRTKKPIAGVIGPGSSSVAIQVQNLLQLFDIPQIAYSATSIDLSDKTLYKYFLRVVPSDTL
  QARAMLDIVKRYNWTYVSAVHTEGNYGESGMDAFKELAAQEGLCIAHSDKIYSNAGEKSFDRLLRKLRER
  LPKARVVVCFCEGMTVRGLLSAMRRLGVVGEFSLIGSDGWADRDEVIEGYEVEANGGITIKLQSPEVRSF
  DDYFLKLRLDTNTRNPWFPEFWQHRFQCRLPGHLLENPNFKRICTGNESLEENYVQDSKMGFVINAIYAM
  AHGLQNMHHALCPGHVGLCDAMKPIDGSKLLDFLIKSSFIGVSGEEVWFDEKGDAPGRYDIMNLQYTEAN
  RYDYVHVGTWHEGVLNIDDYKIQMNKSGVVRSVCSEPCLKGQIKVIRKGEVSCCWICTACKENEYVQDEF
  TCKACDLGWWPNADLTGCEPIPVRYLEWSNIESIIAIAFSCLGILVTLFVTLIFVLYRDTPVVKSSSREL
  CYIILAGIFLGYVCPFTLIAKPTTTSCYLQRLLVGLSSAMCYSALVTKTNRIARILAGSKKKICTRKPRF
  MSAWAQVIIASILISVQLTLVVTLIIMEPPMPILSYPSIKEVYLICNTSNLGVVAPLGYNGLLIMSCTYY
  AFKTRNVPANFNEAKYIAFTMYTTCIIWLAFVPIYFGSNYKIITTCFAVSLSVTVALGCMFTPKMYIIIA
  KPERNVRSAFTTSDVVRMHVGDGKLPCRSNTFLNIFRRKKAGAGNANSNGKSVSWSEPGGGQVPKGQHMW
  HRLSVHVKTNETACNQTAVIKPLTKSYQGSGKSLTFSDTSTKTLYNVEEEEDAQPIRFSPPGSPSMVVHR
  RVPSAATTPPLPSHLTAEETPLFLAEPALPKGLPPPLQQQQQPPPQQKSLMDQLQGVVSNFSTAIPDFHA
  VLAGPGGPGNGLRSLYPPPPPPQHLQMLPLQLSTFGEELVSPPADDDDDSERFKLLQEYVYEHEREGNTE
  EDELEEEEEDLQAASKLTPDDSPALTPPSPFRDSVASGSSVPSSPVSESVLCTPPNVSYASVILRDYKQS
  SSTL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000838
• ORF Size: 3582 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000838.2, NP_000829.1
• RefSeq Size: 6619 bp
• RefSeq ORF: 3584 bp
• Locus ID: 2911
• Cytogenetics: 6q24.3
• Domains: 7tm_3, ANF_receptor
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Calcium signaling pathway, Gap junction, Long-term depression, Long-term potentiation, Neuroactive ligand-receptor interaction
• Gene Summary: This gene encodes a metabotropic glutamate receptor that functions by activating phospholipase C. L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The canonical alpha isoform of the encoded protein is a disulfide-linked homodimer whose activity is mediated by a G-protein-coupled phosphatidylinositol-calcium second messenger system. This gene may be associated with many disease states, including schizophrenia, bipolar disorder, depression, and breast cancer. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, May 2013]