HOXB1 (NM_002144) Human Tagged ORF Clone

CAT#: RG214217

HOXB1 (tGFP-tagged) - Human homeobox B1 (HOXB1)

Product Images

Circular map for RG214217

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: HOXB1
• Synonyms: HCFP3; Hox-2.9; HOX2; HOX2I
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG214217 representing NM_002144
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTATAATAGGATGAACTCCTTCTTAGAGTACCCACTCTGTAACCGGGGACCCAGCGCCTACAGCG
  CCCACAGCGCCCCAACCTCCTTTCCCCCAAGCTCGGCTCAGGCGGTTGACAGCTATGCAAGCGAGGGCCG
  CTACGGTGGGGGGCTGTCCAGCCCTGCGTTTCAGCAGAACTCCGGCTATCCCGCCCAGCAGCCGCCTTCG
  ACCCTGGGGGTGCCCTTCCCCAGCTCCGCGCCCTCGGGGTATGCTCCTGCCGCCTGCAGCCCCAGCTACG
  GGCCTTCTCAGTACTACCCTCTGGGTCAATCAGAAGGAGACGGAGGCTATTTTCATCCCTCGAGCTACGG
  GGCCCAGCTAGGGGGCTTGTCCGATGGCTACGGAGCAGGTGGAGCCGGTCCGGGGCCATATCCTCCGCAG
  CATCCCCCTTATGGGAACGAGCAGACCGCGAGCTTTGCACCGGCCTATGCTGATCTCCTCTCCGAGGACA
  AGGAAACACCCTGCCCTTCAGAACCTAACACCCCCACGGCCCGGACCTTCGACTGGATGAAGGTTAAGAG
  AAACCCACCCAAGACAGCGAAGGTGTCAGAGCCAGGCCTGGGCTCGCCCAGTGGCCTCCGCACCAACTTC
  ACCACAAGGCAGCTGACAGAACTGGAAAAGGAGTTCCATTTCAACAAGTACCTGAGCCGGGCCCGGAGGG
  TGGAGATTGCCGCCACCCTGGAGCTCAATGAAACACAGGTCAAGATTTGGTTCCAGAACCGACGAATGAA
  GCAGAAGAAGCGCGAGCGAGAGGAAGGTCGGGTCCCCCCAGCCCCACCAGGCTGCCCCAAGGAGGCAGCT
  GGAGATGCCTCAGACCAGTCGACATGCACCTCCCCGGAAGCCTCACCCAGCTCTGTCACCTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG214217 representing NM_002144
  Red=Cloning site Green=Tags(s)
  
  MDYNRMNSFLEYPLCNRGPSAYSAHSAPTSFPPSSAQAVDSYASEGRYGGGLSSPAFQQNSGYPAQQPPS
  TLGVPFPSSAPSGYAPAACSPSYGPSQYYPLGQSEGDGGYFHPSSYGAQLGGLSDGYGAGGAGPGPYPPQ
  HPPYGNEQTASFAPAYADLLSEDKETPCPSEPNTPTARTFDWMKVKRNPPKTAKVSEPGLGSPSGLRTNF
  TTRQLTELEKEFHFNKYLSRARRVEIAATLELNETQVKIWFQNRRMKQKKREREEGRVPPAPPGCPKEAA
  GDASDQSTCTSPEASPSSVTS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002144
• ORF Size: 903 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002144.4
• RefSeq Size: 1014 bp
• RefSeq ORF: 906 bp
• Locus ID: 3211
• UniProt ID: P14653
• Cytogenetics: 17q21.32
• Protein Families: Transcription Factors
• Gene Summary: This gene belongs to the homeobox family of genes. The homeobox genes encode a highly conserved family of transcription factors that play an important role in morphogenesis in all multicellular organisms. Mammals possess four similar homeobox gene clusters, HOXA, HOXB, HOXC and HOXD, located on different chromosomes, consisting of 9 to 11 genes arranged in tandem. This gene is one of several homeobox HOXB genes located in a cluster on chromosome 17. [provided by RefSeq, Jul 2008]