Bombesin Receptor 3 (BRS3) (NM_001727) Human Tagged ORF Clone

CAT#: RG213923

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  • TrueORF®

BRS3 (GFP-tagged) - Human bombesin-like receptor 3 (BRS3)



  "NM_001727" in other vectors (6)

USD 460.00


Availability*
In Stock

Size
    • 10 ug


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Specifications

Product Data
Product Name Bombesin Receptor 3 (BRS3) (NM_001727) Human Tagged ORF Clone
Type Human Tagged ORF Clone
Tag TurboGFP
Symbol BRS3
Synonyms BB3; BB3R; BBR3
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Cell Selection Neomycin
Sequence Data
>RG213923 representing NM_001727
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTCAAAGGCAGCCTCACTCACCTAATCAGACTTTAATTTCAATCACAAATGACACAGAATCATCAA
GCTCTGTGGTTTCTAACGATAACACAAATAAAGGATGGAGCGGGGACAACTCTCCAGGAATAGAAGCATT
GTGTGCCATCTATATTACTTATGCTGTGATCATTTCAGTGGGCATCCTTGGAAATGCTATTCTCATCAAA
GTCTTTTTCAAGACCAAATCCATGCAAACAGTTCCAAATATTTTCATCACCAGCCTGGCTTTTGGAGATC
TTTTACTTCTGCTAACTTGTGTGCCAGTGGATGCAACTCACTACCTTGCAGAAGGATGGCTGTTCGGAAG
AATTGGTTGTAAGGTGCTCTCTTTCATCCGGCTCACTTCTGTTGGTGTGTCAGTGTTCACATTAACAATT
CTCAGCGCTGACAGATACAAGGCAGTTGTGAAGCCACTTGAGCGACAGCCCTCCAATGCCATCCTGAAGA
CTTGTGTAAAAGCTGGCTGCGTCTGGATCGTGTCTATGATATTTGCTCTACCTGAGGCTATATTTTCAAA
TGTATACACTTTTCGAGATCCCAATAAAAATATGACATTTGAATCATGTACCTCTTATCCTGTCTCTAAG
AAGCTCTTGCAAGAAATACATTCTCTGCTGTGCTTCTTAGTGTTCTACATTATTCCACTCTCTATTATCT
CTGTCTACTATTCCTTGATTGCTAGGACCCTTTACAAAAGCACCCTGAACATACCTACTGAGGAACAAAG
CCATGCCCGTAAGCAGATTGAATCCCGAAAGAGAATTGCCAGAACGGTATTGGTGTTGGTGGCTCTGTTT
GCCCTCTGCTGGTTGCCAAATCACCTCCTGTACCTCTACCATTCATTCACTTCTCAAACCTATGTAGACC
CCTCTGCCATGCATTTCATTTTCACCATTTTCTCTCGGGTTTTGGCTTTCAGCAATTCTTGCGTAAACCC
CTTTGCTCTCTACTGGCTGAGCAAAAGCTTCCAGAAGCATTTTAAAGCTCAGTTGTTCTGTTGCAAGGCG
GAGCGGCCTGAGCCTCCTGTTGCTGACACCTCTCTTACCACCCTGGCTGTGATGGGAACGGTCCCGGGCA
CTGGGAGCATACAGATGTCTGAAATTAGTGTGACCTCGTTCACTGGGTGTAGTGTGAAGCAGGCAGAGGA
CAGATTC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG213923 representing NM_001727
Red=Cloning site Green=Tags(s)

MAQRQPHSPNQTLISITNDTESSSSVVSNDNTNKGWSGDNSPGIEALCAIYITYAVIISVGILGNAILIK
VFFKTKSMQTVPNIFITSLAFGDLLLLLTCVPVDATHYLAEGWLFGRIGCKVLSFIRLTSVGVSVFTLTI
LSADRYKAVVKPLERQPSNAILKTCVKAGCVWIVSMIFALPEAIFSNVYTFRDPNKNMTFESCTSYPVSK
KLLQEIHSLLCFLVFYIIPLSIISVYYSLIARTLYKSTLNIPTEEQSHARKQIESRKRIARTVLVLVALF
ALCWLPNHLLYLYHSFTSQTYVDPSAMHFIFTIFSRVLAFSNSCVNPFALYWLSKSFQKHFKAQLFCCKA
ERPEPPVADTSLTTLAVMGTVPGTGSIQMSEISVTSFTGCSVKQAEDRF

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
Tag TurboGFP
ACCN NM_001727
ORF Size 1197 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001727.1, NP_001718.1
RefSeq Size 1413 bp
RefSeq ORF 1200 bp
Locus ID 680
Cytogenetics Xq26.3
Protein Families Druggable Genome, GPCR, Transmembrane
Protein Pathways Neuroactive ligand-receptor interaction
Gene Summary The protein encoded by this gene is a G protein-coupled membrane receptor that binds bombesin-like peptides. This binding results in activation of a phosphatidylinositol-calcium second messenger system, with physiological effects including regulation of metabolic rate, glucose metabolism, and hypertension. [provided by RefSeq, Sep 2011]

Citations (1)

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