THOC5 (NM_001002877) Human Tagged ORF Clone

CAT#: RG213773

THOC5 (tGFP-tagged) - Human THO complex 5 (THOC5), transcript variant 3

Product Images

Circular map for RG213773

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: THOC5
• Synonyms: C22orf19; Fmip; fSAP79; PK1.3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG213773 representing NM_001002877
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCATCAGAATCGAGCAAAAAACGGAAGCCCAAAGTGATCCGAAGCGATGGAGCCCCAGCTGAAGGAA
  AGCGGAATCGATCTGACACCGAGCAGGAAGGTAAATACTACAGTGAGGAGGCCGAGGTGGATCTGCGGGA
  CCCTGGCAGAGACTATGAGTTATACAAGTACACCTGCCAGGAGCTACAGAGGCTGATGGCTGAGATCCAA
  GACCTGAAGAGCAGGGGTGGCAAGGATGTGGCAATAGAAATAGAAGAACGGAGGATCCAGAGCTGTGTGC
  ATTTCATGACTCTAAAGAAGCTTAACCGATTAGCCCACATCAGGTTGAAGAAAGGAAGAGATCAGACCCA
  CGAGGCTAAGCAGAAAGTAGATGCCTATCATCTGCAGCTCCAGAACCTGTTGTATGAGGTGATGCACCTA
  CAGAAGGAGATCACCAAATGTTTGGAGTTTAAGTCAAAGCATGAAGAAATTGATCTGGTCAGTTTAGAGG
  AGTTTTATAAGGAGGCTCCACCAGATATCAGCAAGGCCGAAGTCACCATGGGAGACCCTCACCAGCAAAC
  ACTGGCACGTCTGGACTGGGAGCTGGAGCAGCGGAAAAGGCTGGCAGAGAAGTACCGAGAGTGCCTATCT
  AACAAGGAGAAGATTCTCAAGGAGATTGAGGTGAAGAAGGAGTACCTGAGCAGCCTCCAGCCCCGCCTCA
  ACAGCATCATGCAGGCTTCCCTTCCGGTGCAGGAGTACCTGTTTATGCCATTCGACCAGGCTCACAAGCA
  GTATGAGACAGCCAGACACCTGCCGCCTCCCCTCTATGTCCTCTTTGTTCAGGCCACTGCGTATGGGCAG
  GCCTGTGATAAGACGTTATCTGTGGCAATCGAAGGCAGTGTGGATGAAGCCAAGGCTCTGTTCAAACCTC
  CAGAGGACTCCCAAGATGACGAGAGTGACTCAGATGCCGAGGAGGAGCAGACTACGAAGCGCCGGAGACC
  CACACTGGGGGTTCAGTTGGACGACAAACGCAAGGAGATGCTGAAGAGGCACCCACTGTCTGTCATGCTC
  GACCTGAAGTGCAAAGATGACAGTGTGCTTCACCTGACTTTCTACTACCTCATGAACCTCAACATCATGA
  CAGTAAAAGCCAAAGTGACAACTGCCATGGAGCTGATCACCCCCATCAGTGCAGGTGACTTGCTGTCTCC
  TGACTCAGTCCTGAGTTGCTTGTATCCTGGGGATCATGGAAAGAAAACTCCGAATCCAGCCAATCAGTAT
  CAGTTTGATAAAGTTGGCATCCTGACTTTGAGCGACTATGTACTTGAGCTAGGTCACCCCTATTTGTGGG
  TGCAGAAGCTGGGTGGCCTCCACTTCCCCAAAGAGCAGCCCCAGCAAACAGTGATTGCTGACCACTCGCT
  GAGCGCCAGCCACATGGAGACCACCATGAAACTTCTGAAGACCAGGGTGCAGTCCCGCCTGGCCCTCCAC
  AAACAGTTTGCATCCCTAGAACATGGCATTGTGCCAGTTACCAGTGATTGCCAGTACCTCTTCCCTGCCA
  AGGTTGTCTCTCGCCTGGTGAAATGGGTGACAGTTGCCCATGAGGATTACATGGAGCTGCACTTCACCAA
  AGACATTGTGGATGCGGGACTGGCTGGGGACACCAATCTCTACTACATGGCGCTCATCGAAAGGGGCACA
  GCCAAACTGCAGGCCGCTGTGGTGTTGAACCCTGGCTACTCCTCCATCCCACCTGTTTTCCAGCTCTGTT
  TGAACTGGAAAGGGGAGAAAACCAACAGCAACGATGACAACATTCGGGCCATGGAGGGCGAAGTCAATGT
  GTGCTACAAGGAGCTGTGTGGCCCTTGGCCCAGCCACCAGCTGTTGACCAACCAGCTGCAGCGGCTGTGT
  GTGCTGCTGGATGTTTACCTGGAGACCGAGAGCCATGACGACAGTGTGGAGGGGCCCAAGGAATTTCCCC
  AGGAGAAGATGTGTCTGCGGCTCTTCAGGGGTCCTAGCAGGATGAAGCCATTTAAATACAACCATCCTCA
  GGGATTCTTCAGCCATCGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG213773 representing NM_001002877
  Red=Cloning site Green=Tags(s)
  
  MSSESSKKRKPKVIRSDGAPAEGKRNRSDTEQEGKYYSEEAEVDLRDPGRDYELYKYTCQELQRLMAEIQ
  DLKSRGGKDVAIEIEERRIQSCVHFMTLKKLNRLAHIRLKKGRDQTHEAKQKVDAYHLQLQNLLYEVMHL
  QKEITKCLEFKSKHEEIDLVSLEEFYKEAPPDISKAEVTMGDPHQQTLARLDWELEQRKRLAEKYRECLS
  NKEKILKEIEVKKEYLSSLQPRLNSIMQASLPVQEYLFMPFDQAHKQYETARHLPPPLYVLFVQATAYGQ
  ACDKTLSVAIEGSVDEAKALFKPPEDSQDDESDSDAEEEQTTKRRRPTLGVQLDDKRKEMLKRHPLSVML
  DLKCKDDSVLHLTFYYLMNLNIMTVKAKVTTAMELITPISAGDLLSPDSVLSCLYPGDHGKKTPNPANQY
  QFDKVGILTLSDYVLELGHPYLWVQKLGGLHFPKEQPQQTVIADHSLSASHMETTMKLLKTRVQSRLALH
  KQFASLEHGIVPVTSDCQYLFPAKVVSRLVKWVTVAHEDYMELHFTKDIVDAGLAGDTNLYYMALIERGT
  AKLQAAVVLNPGYSSIPPVFQLCLNWKGEKTNSNDDNIRAMEGEVNVCYKELCGPWPSHQLLTNQLQRLC
  VLLDVYLETESHDDSVEGPKEFPQEKMCLRLFRGPSRMKPFKYNHPQGFFSHR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001002877
• ORF Size: 2049 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001002877.2
• RefSeq Size: 2563 bp
• RefSeq ORF: 2052 bp
• Locus ID: 8563
• UniProt ID: Q13769
• Cytogenetics: 22q12.2
• Gene Summary: Acts as component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. THOC5 in conjunction with ALYREF/THOC4 functions in NXF1-NXT1 mediated nuclear export of HSP70 mRNA; both proteins enhance the RNA binding activity of NXF1 and are required for NXF1 localization to the nuclear rim. Involved in transcription elongation and genome stability. Involved in alternative polyadenylation site choice by recruiting CPSF6 to 5' region of target genes; probably mediates association of the TREX and CFIm complexes.[UniProtKB/Swiss-Prot Function]