PGAM4 (NM_001029891) Human Tagged ORF Clone

CAT#: RG213480

PGAM4 (tGFP-tagged) - Human phosphoglycerate mutase family member 4 (PGAM4)

Product Images

Circular map for RG213480

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PGAM4
• Synonyms: dJ1000K24.1; PGAM-B; PGAM1; PGAM3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG213480 representing NM_001029891
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGCCTACAAACTGGTGCTGATCCGGCACGGCGAGAGCACATGGAACCTGGAGAACCGCTTCAGCT
  GCTGGTACGACGCCGATCTGAGCCCGGCGGGCCACGAGGAGGCGAAGCGCGGCGGGCAGGCGCTACGAGA
  TGCTGGCTATGAGTTTGACATCTGCCTCACCTCAGTGCAGAAGAGAGTGATCCGGACCCTCTGGACAGTG
  CTAGATGCCATTGATCAGATGTGGCTGCCAGTGGTGAGGACTTGGCGCCTCAATGAGCGGCACTATGGGG
  GTCTAACCGGTCTCAATAAAGCAGAAACTGCTGCAAAGCATGGTGAGGCCCAGGTGAAGATCTGGAGGCG
  CTCCTATGATGTCCCACCACCTCCGATGGAGCCCGACCATCCTTTCTACAGCAACATCAGTAAGGATCGC
  AGGTATGCAGACCTCACAGAAGATCAGCTACCCTCCTATGAGAGTCCGAAGGATACTATTGCCAGAGCTC
  TGCCCTTCTGGAATGAAGAAATAGTTCCCCAGATCAAGGAGGGGAAACGTGTACTGATTGCAGCCCATGG
  CAACAGCCTCCAGGGCATTGCCAAGCATGTGGAGGGTCTCTCTGAAGAGGCTATCATGGAGCTGAACCTG
  CCGACTGGTATTCCCATCGTCTATGAATTGGACAAGAACTTGAAGCCTATCAAGCCCATGCAGTTTCTGG
  GGGATGAAGAGACGGTGTGCAAAGCCATAGAAGCTGTGGCTGCCCAGGGCAAGGCCAAGAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG213480 representing NM_001029891
  Red=Cloning site Green=Tags(s)
  
  MAAYKLVLIRHGESTWNLENRFSCWYDADLSPAGHEEAKRGGQALRDAGYEFDICLTSVQKRVIRTLWTV
  LDAIDQMWLPVVRTWRLNERHYGGLTGLNKAETAAKHGEAQVKIWRRSYDVPPPPMEPDHPFYSNISKDR
  RYADLTEDQLPSYESPKDTIARALPFWNEEIVPQIKEGKRVLIAAHGNSLQGIAKHVEGLSEEAIMELNL
  PTGIPIVYELDKNLKPIKPMQFLGDEETVCKAIEAVAAQGKAKK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001029891
• ORF Size: 762 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001029891.3
• RefSeq Size: 1678 bp
• RefSeq ORF: 765 bp
• Locus ID: 441531
• UniProt ID: Q8N0Y7
• Cytogenetics: Xq21.1
• Protein Pathways: Glycolysis / Gluconeogenesis, Metabolic pathways
• Gene Summary: This intronless gene appears to have arisen from a retrotransposition event, yet it is thought to be an expressed, protein-coding gene. The encoded protein is a member of the phosphoglycerate mutase family, a set of enzymes that catalyze the transfer of a phosphate group from 3-phosphoglycerate to 2-phosphoglycerate. [provided by RefSeq, May 2010]