HOXA4 (NM_002141) Human Tagged ORF Clone

CAT#: RG213447

HOXA4 (tGFP-tagged) - Human homeobox A4 (HOXA4)

Product Images

Circular map for RG213447

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: HOXA4
• Synonyms: HOX1; HOX1D
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG213447 representing NM_002141
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCATGAGCTCGTTTTTGATAAACTCCAACTACATCGAGCCCAAGTTCCCTCCCTTCGAGGAGTACG
  CGCAGCACAGCGGCTCGGGCGGCGCAGACGGCGGCCCGGGCGGGGGCCCCGGCTACCAGCAGCCCCCAGC
  GCCCCCGACCCAGCACCTGCCGCTGCAGCAGCCCCAGCTCCCTCACGCGGGCGGCGGCCGAGAGCCCACT
  GCCTCCTACTACGCGCCGCGGACCGCCCGCGAGCCCGCCTACCCTGCTGCCGCGCTGTACCCCGCGCATG
  GGGCCGCGGACACCGCCTACCCCTATGGCTACCGCGGCGGCGCCAGCCCCGGGCGGCCGCCCCAGCCCGA
  GCAGCCCCCGGCGCAAGCCAAGGGCCCAGCGCACGGCCTGCATGCGAGCCACGTCCTGCAGCCCCAGCTG
  CCGCCGCCCCTGCAGCCTCGCGCCGTGCCCCCAGCGGCCCCGCGGCGCTGCGAGGCGGCCCCCGCCACCC
  CAGGCGTCCCGGCAGGGGGCAGCGCCCCCGCGTGCCCGCTGCTCTTGGCCGACAAGAGCCCGCTGGGCCT
  GAAGGGCAAGGAGCCCGTGGTGTACCCCTGGATGAAGAAGATCCATGTCAGCGCCGTTAACCCCAGTTAT
  AACGGAGGGGAGCCTAAGCGCTCTCGAACCGCCTACACCCGGCAGCAGGTCTTGGAGCTGGAGAAGGAGT
  TCCACTTCAATCGATACCTGACCCGGCGGCGCCGCATCGAGATCGCCCACACGCTCTGTTTGTCTGAGCG
  CCAGGTCAAGATCTGGTTTCAGAACCGGAGGATGAAGTGGAAGAAAGACCACAAACTGCCCAACACCAAG
  ATGCGATCCTCCAATTCGGCCTCGGCCTCTGCCGGCCCACCAGGGAAAGCACAAACTCAGAGCCCACACC
  TCCATCCCCACCCCCACCCGAGCACCTCCACACCCGTTCCCTCCTCCATA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG213447 representing NM_002141
  Red=Cloning site Green=Tags(s)
  
  MTMSSFLINSNYIEPKFPPFEEYAQHSGSGGADGGPGGGPGYQQPPAPPTQHLPLQQPQLPHAGGGREPT
  ASYYAPRTAREPAYPAAALYPAHGAADTAYPYGYRGGASPGRPPQPEQPPAQAKGPAHGLHASHVLQPQL
  PPPLQPRAVPPAAPRRCEAAPATPGVPAGGSAPACPLLLADKSPLGLKGKEPVVYPWMKKIHVSAVNPSY
  NGGEPKRSRTAYTRQQVLELEKEFHFNRYLTRRRRIEIAHTLCLSERQVKIWFQNRRMKWKKDHKLPNTK
  MRSSNSASASAGPPGKAQTQSPHLHPHPHPSTSTPVPSSI
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002141
• ORF Size: 960 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002141.5
• RefSeq Size: 1728 bp
• RefSeq ORF: 963 bp
• Locus ID: 3201
• UniProt ID: Q00056
• Cytogenetics: 7p15.2
• Protein Families: Transcription Factors
• Gene Summary: In vertebrates, the genes encoding the class of transcription factors called homeobox genes are found in clusters named A, B, C, and D on four separate chromosomes. Expression of these proteins is spatially and temporally regulated during embryonic development. This gene is part of the A cluster on chromosome 7 and encodes a DNA-binding transcription factor which may regulate gene expression, morphogenesis, and differentiation. [provided by RefSeq, Jul 2008]