ZIP Kinase (DAPK3) (NM_001348) Human Tagged ORF Clone

CAT#: RG213427

DAPK3 (tGFP-tagged) - Human death-associated protein kinase 3 (DAPK3)

Product Images

Circular map for RG213427

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ZIP Kinase
• Synonyms: DLK; ZIP; ZIPK
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG213427 representing NM_001348
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCACGTTCAGGCAGGAGGACGTGGAGGACCATTATGAGATGGGGGAGGAGCTGGGCAGCGGCCAGT
  TTGCGATCGTGCGGAAGTGCCGGCAGAAGGGCACGGGCAAGGAGTACGCAGCCAAGTTCATCAAGAAGCG
  CCGCCTGTCATCCAGCCGGCGTGGGGTGAGCCGGGAGGAGATCGAGCGGGAGGTGAACATCCTGCGGGAG
  ATCCGGCACCCCAACATCATCACCCTGCACGACATCTTCGAGAACAAGACGGACGTGGTCCTCATCCTGG
  AGCTGGTCTCTGGCGGGGAGCTCTTTGACTTCCTGGCGGAGAAGGAGTCGCTGACGGAGGACGAGGCCAC
  CCAGTTCCTCAAGCAGATCCTGGACGGCGTTCACTACCTGCACTCTAAGCGCATCGCACACTTTGACCTG
  AAGCCGGAAAACATCATGCTGCTGGACAAGAACGTGCCCAACCCACGAATCAAGCTCATCGACTTCGGCA
  TCGCGCACAAGATCGAGGCGGGGAACGAGTTCAAGAACATCTTCGGCACCCCGGAGTTTGTGGCCCCAGA
  GATTGTGAACTATGAGCCGCTGGGCCTGGAGGCGGACATGTGGAGCATCGGTGTCATCACCTATATCCTC
  CTGAGCGGTGCATCCCCGTTCCTGGGCGAGACCAAGCAGGAGACGCTCACCAACATCTCAGCCGTGAACT
  ACGACTTCGACGAGGAGTACTTCAGCAACACCAGCGAGCTGGCCAAGGACTTCATTCGCCGGCTGCTCGT
  CAAAGATCCCAAGCGGAGAATGACCATTGCCCAGAGCCTGGAACATTCCTGGATTAAGGCGATCCGGCGG
  CGGAACGTGCGTGGTGAGGACAGCGGCCGCAAGCCCGAGCGGCGGCGCCTGAAGACCACGCGTCTGAAGG
  AGTACACCATCAAGTCGCACTCCAGCTTGCCGCCCAACAACAGCTACGCCGACTTCGAGCGCTTCTCCAA
  GGTGCTGGAGGAGGCGGCGGCCGCCGAGGAGGGCCTGCGCGAGCTGCAGCGCAGCCGGCGGCTCTGCCAC
  GAGGACGTGGAGGCGCTGGCCGCCATCTACGAGGAGAAGGAGGCCTGGTACCGCGAGGAGAGCGACAGCC
  TGGGCCAGGACCTGCGGAGGCTACGGCAGGAGCTGCTCAAGACCGAGGCGCTCAAGCGGCAGGCGCAGGA
  GGAGGCCAAGGGCGCGCTGCTGGGGACCAGCGGCCTCAAGCGCCGCTTCAGCCGCCTGGAGAACCGCTAC
  GAGGCGCTGGCCAAGCAAGTAGCCTCCGAGATGCGCTTCGTGCAGGACCTCGTGCGCGCCCTGGAGCAGG
  AGAAGCTGCAGGGCGTGGAGTGCGGGCTGCGC
  
  CTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG213427 representing NM_001348
  Red=Cloning site Green=Tags(s)
  
  MSTFRQEDVEDHYEMGEELGSGQFAIVRKCRQKGTGKEYAAKFIKKRRLSSSRRGVSREEIEREVNILRE
  IRHPNIITLHDIFENKTDVVLILELVSGGELFDFLAEKESLTEDEATQFLKQILDGVHYLHSKRIAHFDL
  KPENIMLLDKNVPNPRIKLIDFGIAHKIEAGNEFKNIFGTPEFVAPEIVNYEPLGLEADMWSIGVITYIL
  LSGASPFLGETKQETLTNISAVNYDFDEEYFSNTSELAKDFIRRLLVKDPKRRMTIAQSLEHSWIKAIRR
  RNVRGEDSGRKPERRRLKTTRLKEYTIKSHSSLPPNNSYADFERFSKVLEEAAAAEEGLRELQRSRRLCH
  EDVEALAAIYEEKEAWYREESDSLGQDLRRLRQELLKTEALKRQAQEEAKGALLGTSGLKRRFSRLENRY
  EALAKQVASEMRFVQDLVRALEQEKLQGVECGLR
  
  LE - GFP Tag - V
• Restriction Sites: SgfI-XhoI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001348
• ORF Size: 1362 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001348.3
• RefSeq Size: 2105 bp
• RefSeq ORF: 1365 bp
• Locus ID: 1613
• UniProt ID: O43293
• Cytogenetics: 19p13.3
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Bladder cancer, Pathways in cancer
• Gene Summary: Death-associated protein kinase 3 (DAPK3) induces morphological changes in apoptosis when overexpressed in mammalian cells. These results suggest that DAPK3 may play a role in the induction of apoptosis. [provided by RefSeq, Jul 2008]