MINAR1 (NM_015206) Human Tagged ORF Clone

CAT#: RG213413

KIAA1024 (tGFP-tagged) - Human KIAA1024 (KIAA1024)

Product Images

Circular map for RG213413

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: MINAR1
• Synonyms: KIAA1024; UBTOR
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG213413 representing NM_015206
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGACCAGTCAGGAAACTTCCCTCTTCTTGGTGAAGATCTTGGAGGAACTGGACAGCAAGCAAAATA
  CCGTTTCTTATCAGGACCTGTGCAAATCTCTCTGTGCCCGGTTCGACCTGTCGCAGCTTGCCAAACTGAG
  AAGTGTGCTCTTCTACACAGCTTGTCTCGATCCCAATTTTCCAGCCACGCTATTCAAAGACAAGATGAAA
  TGCACTGTGAATAACCAGCAATCAAAGAAAATCATGGTGGCAGCAGATATTGTGACGATATTCAACCTGA
  TCCAAATGAATGGCGGGGCTGCCAAGGAGAAGCTGCCCACGGGCCGCCAGAAGGTACGCAAGAAGGAGGC
  ATCCTTTGAATCATGTAGGTCGGACACAGAGATCTGCAATGCAGCTGAGTGTGAGCCCCTGAACTGTGAG
  CTGAGTGAGAGGTCTTTCAGCCGGGGCTACCCCATCAGGCAGTCGTCCAAGTGCCGGAAGATGGACTGCA
  AGGACTGCCCACAGTTTGTCCCTGCCTCTGAGCCTAACTTCCTGTTGGGAGTTAGCAAAGAGGTGAAAAA
  CCGCGCCGCTTCCCTGGACAGGTTGCAGGCCCTGGCTCCGTACTCTGTGACCAGCCCTCAGCCCTGTGAG
  ATGCAGAGGACCTACTTCCCCATGAACATCGAAAACGAGTCCATTTCAGACCAGGACTCCCTGCCCATCA
  ATCAGAGCATCAAGGAGACCTTCATTTCCAATGAGGAGCCATTTGTGGTCCAGTCCTGTGTCCAGAAAAG
  GAATATCTTCAAAGAGGATTTTCACAATTTGATGGCAGTGTCCCCCAGTTTGGTTGGCCCCATCAGCAAA
  GCAGAGAATGAGCACAGGGAACCCCAGAGTCGAAAGGAACCCCACAAGCCACCCTTCTTCAACCACAGCT
  TTGAAATGCCCTATAACAGCCAGTACCTGAATCCAGTGTATTCCCCGGTTCCTGACAAAAGGCGAGCAAA
  GCACGAAAGCTTAGATGACCTTCAAGCCTCTACATATTTTGGGCCCACTCCCGTGATGGGAACCCAAGAA
  GCCAGGCGCTGTCTAGGGAAGCCCAACAAGCAGACTCCCTGGCCAGCCAAAAGCTGGAGCCTAAACACAG
  AGGAAGTTCCTGACTTTGAACGGTCCTTTTTCAATAGAAATCCCTCCGAGGAGAAGCTACACTATCCAAA
  TGCCAGTAGCCAGACCCCCAATTTCCCAGCCCCAGAAAGGCGCCCAACTTACCTTGTGCCAAAGGATCAA
  CAGCCAATTCTCCCCATTGCTTATGCGGCAAAACAAAATGGGCTCAAATCTAAAGAGATCTCATCCCCTG
  TTGACCTGGAGAAGCATGAACCAGTCAAAAAGTTTAAAGACAAGAGCATTAACTGCACCAGTGGGCAGCT
  CAGCTCAGACACCAGTAGCGTGGGCACCCAGACTGAGCACGTGCTGGAGCCCAAGAAATGCAGAGACCTG
  TGCACCTCTGGTCAGGGCAAGTACAGTGACAGGCACACCATGAAGCACTCAGACGATGACTCAGAAATTG
  TCAGCGACGACATCAGTGACATTTTCCGATTTCTTGATGACATGAGCATCAGTGGCTCCACGGGAGTGAT
  ACAGTCGTCCTGCTACAACAGCACAGGATCCTTGTCTCAGCTCCATAAGTCAGACTGCGACAGTTCCCCT
  GAGCACAACTTAACCAAAATTGCCAATGGGGTCCCCAACAGCAAGGGAGACAAGGGCAACCGGCCTGAAA
  ACACCCACCACTCGGAAGAAGAGCTGAAGACCAGTGTGTGCAAACTGGTGCTCAGGATTGGCGAAATCGA
  ACGGAAGCTGGAATCCCTGTCGGGTGTCCGTGATGAAATCTCCCAGGTCTTGGGCAAACTAAATAAATTG
  GACCAGAAAATGCAACAGCCTGAGAAGGTGAGTGTGCAGATAGATCTGAACTCCTTGACAAGCGAGGGTC
  CGTCTGATGACAGTGCCTCTCCCCGGATGTTCCACGCACACAGTGGCTCCCACGGACCCAAACTAGAGAA
  CAACCCTGACTGGTGCTGCTCTGATGCTAGCGGGAGCAACAGTGAAAGCCTGCGGGTCAAGGCCTTAAAA
  AAAAGCCTCTTCACCAGGCCATCCTCTAGGTCCCTAACAGAGGAGAACAGTGCCACAGAGTCCAAAATTG
  CCAGCATCTCCAACTCGCCCAGAGACTGGCGCACCATCACTTATACCAACCGTGTGGGCCTCAATGAGGA
  GGAGATAAAAGACACAGGCCCAGGAGATAATAAAGACTGGCATCGGAAATCTAAAGAGGCAGACAGGCAG
  TACGACATTCCCCCACAGCACCGACTGCCCAAGCAGCCCAAAGATGGCTTCCTGGTGGAGCAGGTGTTCA
  GCCCTCACCCCTACCCTGCCTCCCTCAAGGCCCACATGAAGAGCAACCCCCTGTACACAGACATGCGGCT
  GACCGAGTTGGCCGAGGTGAAGCGGGGCCAACCTTCTTGGACCATTGAGGAGTATGCACGGAATGCGGGC
  GACAAGGGCAAGCTGACAGCCCTGGACCTGCAGACGCAAGAATCTTTAAACCCAAATAATTTAGAGTACT
  GGATGGAAGACATTTATACTCCAGGATACGATTCATTACTAAAACGTAAAGAAGCCGAATTCAGACGAGC
  CAAGGTCTGCAAGATAGCTGCTCTGATCGCTGCTGCGGCATGCACCGTCATCCTCGTTATTGTCGTGCCC
  ATCTGCACAATGAAATCA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG213413 representing NM_015206
  Red=Cloning site Green=Tags(s)
  
  METSQETSLFLVKILEELDSKQNTVSYQDLCKSLCARFDLSQLAKLRSVLFYTACLDPNFPATLFKDKMK
  CTVNNQQSKKIMVAADIVTIFNLIQMNGGAAKEKLPTGRQKVRKKEASFESCRSDTEICNAAECEPLNCE
  LSERSFSRGYPIRQSSKCRKMDCKDCPQFVPASEPNFLLGVSKEVKNRAASLDRLQALAPYSVTSPQPCE
  MQRTYFPMNIENESISDQDSLPINQSIKETFISNEEPFVVQSCVQKRNIFKEDFHNLMAVSPSLVGPISK
  AENEHREPQSRKEPHKPPFFNHSFEMPYNSQYLNPVYSPVPDKRRAKHESLDDLQASTYFGPTPVMGTQE
  ARRCLGKPNKQTPWPAKSWSLNTEEVPDFERSFFNRNPSEEKLHYPNASSQTPNFPAPERRPTYLVPKDQ
  QPILPIAYAAKQNGLKSKEISSPVDLEKHEPVKKFKDKSINCTSGQLSSDTSSVGTQTEHVLEPKKCRDL
  CTSGQGKYSDRHTMKHSDDDSEIVSDDISDIFRFLDDMSISGSTGVIQSSCYNSTGSLSQLHKSDCDSSP
  EHNLTKIANGVPNSKGDKGNRPENTHHSEEELKTSVCKLVLRIGEIERKLESLSGVRDEISQVLGKLNKL
  DQKMQQPEKVSVQIDLNSLTSEGPSDDSASPRMFHAHSGSHGPKLENNPDWCCSDASGSNSESLRVKALK
  KSLFTRPSSRSLTEENSATESKIASISNSPRDWRTITYTNRVGLNEEEIKDTGPGDNKDWHRKSKEADRQ
  YDIPPQHRLPKQPKDGFLVEQVFSPHPYPASLKAHMKSNPLYTDMRLTELAEVKRGQPSWTIEEYARNAG
  DKGKLTALDLQTQESLNPNNLEYWMEDIYTPGYDSLLKRKEAEFRRAKVCKIAALIAAAACTVILVIVVP
  ICTMKS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015206
• ORF Size: 2748 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015206.1, NP_056021.1
• RefSeq Size: 6741 bp
• RefSeq ORF: 2751 bp
• Locus ID: 23251
• UniProt ID: Q9UPX6
• Cytogenetics: 15q25.1
• Protein Families: Transmembrane
• Gene Summary: Intrinsically disordered protein which may negatively regulate mTOR signaling pathway by stabilizing the mTOR complex component DEPTOR (PubMed:30080879). Negatively regulates angiogenesis (PubMed:29329397). Negatively regulates cell growth (PubMed:29329397, PubMed:30080879). Negatively regulates neurite outgrowth in hippocampal neurons (By similarity).[UniProtKB/Swiss-Prot Function]