TMEM16A (ANO1) (NM_018043) Human Tagged ORF Clone

CAT#: RG213098

ANO1 (tGFP-tagged) - Human anoctamin 1, calcium activated chloride channel (ANO1), transcript variant 1

Product Images

Circular map for RG213098

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: TMEM16A
• Synonyms: DOG1; ORAOV2; TAOS2; TMEM16A
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG213098 representing NM_018043
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGGTCAACGAGAAGTACTCGACGCTCCCGGCCGAGGACCGCAGCGTCCACATCATCAACATCTGCG
  CCATCGAGGACATCGGCTACCTGCCGTCCGAGGGCACGCTGCTGAACTCCTTATCTGTGGACCCTGATGC
  CGAGTGCAAGTATGGCCTGTACTTCAGGGACGGCCGGCGCAAGGTGGACTACATCCTGGTGTACCATCAC
  AAGAGGCCCTCGGGCAACCGGACCCTGGTCAGGAGGGTGCAGCACAGCGACACCCCCTCTGGGGCTCGCA
  GCGTCAAGCAGGACCACCCCCTGCCGGGCAAGGGGGCGTCGCTGGATGCAGGCTCGGGGGAGCCCCCGAT
  GGACTACCACGAGGATGACAAGCGCTTCCGCAGGGAGGAGTACGAGGGCAACCTCCTGGAGGCGGGCCTG
  GAGCTGGAGCGGGACGAGGACACTAAAATCCACGGAGTCGGGTTTGTGAAAATCCATGCCCCCTGGAACG
  TGCTGTGCAGAGAGGCCGAGTTTCTGAAACTGAAGATGCCGACGAAGAAGATGTACCACATTAATGAGAC
  CCGTGGCCTCCTGAAAAAAATCAACTCTGTGCTCCAGAAAATCACAGATCCCATCCAGCCCAAAGTGGCT
  GAGCACAGGCCCCAGACCATGAAGAGACTCTCCTATCCCTTCTCCCGGGAGAAGCAGCATCTATTTGACT
  TGTCTGATAAGGATTCCTTTTTCGACAGCAAAACCCGGAGCACGATTGTCTATGAGATCTTGAAGAGAAC
  GACGTGTACAAAGGCCAAGTACAGCATGGGCATCACGAGCCTGCTGGCCAATGGTGTGTACGCGGCTGCA
  TACCCACTGCACGATGGAGACTACAACGGTGAAAACGTCGAGTTCAACGACAGAAAACTCCTGTACGAAG
  AGTGGGCACGCTATGGAGTTTTCTATAAGTACCAGCCCATCGACCTGGTCAGGAAGTATTTTGGGGAGAA
  GATCGGCCTGTACTTCGCCTGGCTGGGCGTGTACACCCAGATGCTCATCCCTGCCTCCATCGTGGGAATC
  ATTGTCTTCCTGTACGGATGCGCCACCATGGATGAAAACATCCCCAGCATGGAGATGTGTGACCAGAGAC
  ACAATATCACCATGTGCCCGCTTTGCGACAAGACCTGCAGCTACTGGAAGATGAGCTCAGCCTGCGCCAC
  GGCCCGCGCCAGCCACCTCTTCGACAACCCCGCCACGGTCTTCTTCTCTGTCTTCATGGCCCTCTGGGCT
  GCCACCTTCATGGAGCACTGGAAGCGGAAACAGATGCGACTCAACTACCGCTGGGACCTCACGGGCTTTG
  AAGAGGAAGAGGAGGCTGTCAAGGATCATCCTAGAGCTGAATACGAAGCCAGAGTCTTGGAGAAGTCTCT
  GAAGAAAGAGTCCAGAAACAAAGAGACTGACAAAGTGAAGCTGACATGGAGAGATCGGTTCCCAGCCTAC
  CTCACTAACTTGGTCTCCATCATCTTCATGATTGCAGTGACGTTTGCCATCGTCCTCGGCGTCATCATCT
  ACAGAATCTCCATGGCCGCCGCCTTGGCCATGAACTCCTCCCCCTCCGTGCGGTCCAACATCCGGGTCAC
  AGTCACAGCCACCGCAGTCATCATCAACCTAGTGGTCATCATCCTCCTGGACGAGGTGTATGGCTGCATA
  GCCCGATGGCTCACCAAGATCGAGGTCCCAAAGACGGAGAAAAGCTTTGAGGAGAGGCTGATCTTCAAGG
  CTTTCCTGCTGAAGTTTGTGAATTCCTACACCCCCATCTTTTACGTGGCGTTCTTCAAAGGCCGGTTTGT
  TGGACGCCCGGGCGACTACGTGTACATTTTCCGTTCCTTCCGAATGGAAGAGTGTGCGCCAGGGGGCTGC
  CTGATGGAGCTATGCATCCAGCTCAGCATCATCATGCTGGGGAAACAGCTGATCCAGAACAACCTGTTCG
  AGATCGGCATCCCGAAGATGAAGAAGCTCATCCGCTACCTGAAGCTGAAGCAGCAGAGCCCCCCTGACCA
  CGAGGAGTGTGTGAAGAGGAAACAGCGGTACGAGGTGGATTACAACCTGGAGCCCTTCGCGGGCCTCACC
  CCAGAGTACATGGAAATGATCATCCAGTTTGGCTTCGTCACCCTGTTTGTCGCCTCCTTCCCCCTGGCCC
  CACTGTTTGCGCTGCTGAACAACATCATCGAGATCCGCCTGGACGCCAAAAAGTTTGTCACTGAGCTCCG
  AAGGCCGGTAGCTGTCAGAGCCAAAGACATCGGAATCTGGTACAATATCCTCAGAGGCATTGGGAAGCTT
  GCTGTCATCATCAATGCCTTCGTGATCTCCTTCACGTCTGACTTCATCCCGCGCCTGGTGTACCTCTACA
  TGTACAGTAAGAACGGGACCATGCACGGCTTCGTCAACCACACCCTCTCCTCCTTCAACGTCAGTGACTT
  CCAGAACGGCACGGCCCCCAATGACCCCCTGGACCTGGGCTACGAGGTGCAGATCTGCAGGTATAAAGAC
  TACCGAGAGCCGCCGTGGTCGGAAAACAAGTACGACATCTCCAAGGACTTCTGGGCCGTCCTGGCAGCCC
  GGCTGGCGTTTGTCATCGTCTTCCAGAACCTGGTCATGTTCATGAGCGACTTTGTGGACTGGGTCATCCC
  GGACATCCCCAAGGACATCAGCCAGCAGATCCACAAGGAGAAGGTGCTCATGGTGGAGCTGTTCATGCGG
  GAGGAGCAAGACAAGCAGCAGCTGCTGGAAACCTGGATGGAGAAGGAGCGGCAGAAGGACGAGCCGCCGT
  GCAACCACCACAACACCAAAGCCTGCCCAGACAGCCTCGGCAGCCCAGCCCCCAGCCATGCCTACCACGG
  GGGCGTCCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG213098 representing NM_018043
  Red=Cloning site Green=Tags(s)
  
  MRVNEKYSTLPAEDRSVHIINICAIEDIGYLPSEGTLLNSLSVDPDAECKYGLYFRDGRRKVDYILVYHH
  KRPSGNRTLVRRVQHSDTPSGARSVKQDHPLPGKGASLDAGSGEPPMDYHEDDKRFRREEYEGNLLEAGL
  ELERDEDTKIHGVGFVKIHAPWNVLCREAEFLKLKMPTKKMYHINETRGLLKKINSVLQKITDPIQPKVA
  EHRPQTMKRLSYPFSREKQHLFDLSDKDSFFDSKTRSTIVYEILKRTTCTKAKYSMGITSLLANGVYAAA
  YPLHDGDYNGENVEFNDRKLLYEEWARYGVFYKYQPIDLVRKYFGEKIGLYFAWLGVYTQMLIPASIVGI
  IVFLYGCATMDENIPSMEMCDQRHNITMCPLCDKTCSYWKMSSACATARASHLFDNPATVFFSVFMALWA
  ATFMEHWKRKQMRLNYRWDLTGFEEEEEAVKDHPRAEYEARVLEKSLKKESRNKETDKVKLTWRDRFPAY
  LTNLVSIIFMIAVTFAIVLGVIIYRISMAAALAMNSSPSVRSNIRVTVTATAVIINLVVIILLDEVYGCI
  ARWLTKIEVPKTEKSFEERLIFKAFLLKFVNSYTPIFYVAFFKGRFVGRPGDYVYIFRSFRMEECAPGGC
  LMELCIQLSIIMLGKQLIQNNLFEIGIPKMKKLIRYLKLKQQSPPDHEECVKRKQRYEVDYNLEPFAGLT
  PEYMEMIIQFGFVTLFVASFPLAPLFALLNNIIEIRLDAKKFVTELRRPVAVRAKDIGIWYNILRGIGKL
  AVIINAFVISFTSDFIPRLVYLYMYSKNGTMHGFVNHTLSSFNVSDFQNGTAPNDPLDLGYEVQICRYKD
  YREPPWSENKYDISKDFWAVLAARLAFVIVFQNLVMFMSDFVDWVIPDIPKDISQQIHKEKVLMVELFMR
  EEQDKQQLLETWMEKERQKDEPPCNHHNTKACPDSLGSPAPSHAYHGGVL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018043
• ORF Size: 2880 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018043.4, NP_060513.4
• RefSeq Size: 4498 bp
• RefSeq ORF: 2961 bp
• Locus ID: 55107
• UniProt ID: Q5XXA6
• Cytogenetics: 11q13.3
• Domains: DUF590
• Protein Families: Transmembrane
• Gene Summary: Calcium-activated chloride channel (CaCC) which plays a role in transepithelial anion transport and smooth muscle contraction. Required for the normal functioning of the interstitial cells of Cajal (ICCs) which generate electrical pacemaker activity in gastrointestinal smooth muscles. Acts as a major contributor to basal and stimulated chloride conductance in airway epithelial cells and plays an important role in tracheal cartilage development.[UniProtKB/Swiss-Prot Function]