GXYLT1 (NM_001099650) Human Tagged ORF Clone

CAT#: RG213057

GXYLT1 (tGFP-tagged) - Human glucoside xylosyltransferase 1 (GXYLT1), transcript variant 2

Product Images

Circular map for RG213057

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: GXYLT1
• Synonyms: GLT8D3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG213057 representing NM_001099650
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGCGCTACCTGCGCGTCGTGGTGCTGTGTGTGGCCTGCGGCTTCTGCTCGCTCCTTTACGCTTTCA
  GCCAGCTCGCCGTGTCCCTGGAAGAAGGAACGGGCGGCGGTGGCGGGAAGCCGCAGGCCGCGGTGGCTTC
  CTGGCTCGCGGGCGGCGGACGCGGCGCCGTGAGAGGCGCCGGCGTCGCGGGCCCCGCAGCGCATCCCGGC
  GTGTCGGACAGGTACAGTCTGAAAATACAGCCTGTTGAGAAAATGCATCTAGCTGTAGTTGCCTGTGGTG
  AAAGACTGGAAGAAACTATGACCATGTTGAAGTCAGCTATCATTTTCAGCATCAAACCTCTTCAATTCCA
  TATTTTTGCTGAAGATCAGCTACATCATAGCTTTAAAGGCAGACTTGACAACTGGTCATTTCTACAAACA
  TTTAATTATACGTTATACCCCATAACCTTTCCAAGTGAGAATGCAGCAGAGTGGAAAAAACTCTTTAAAC
  CATGTGCTTCGCAGAGATTGTTCTTGCCGTTAATCCTGAAAGAAGTTGACTCACTATTGTATGTCGACAC
  TGATATCCTTTTTTTACGACCAGTTGATGATATTTGGTCTTTACTAAAGAAATTTAATTCCACACAAATT
  GCTGCAATGGCACCAGAACATGAGGAACCTCGAATAGGATGGTATAATCGCTTTGCTAGGCATCCATATT
  ATGGAAAAACTGGAGTAAACTCTGGAGTTATGTTGATGAACATGACTCGAATGAGAAGGAAGTATTTCAA
  GAATGATATGACAACTGTACGACTACAATGGGGAGATATACTTATGCCATTGCTTAAAAAATACAAACTA
  AACATCACATGGGGTGATCAAGATCTATTGAATATCGTGTTTTTTCATAATCCAGAAAGCCTTTTTGTTT
  TTCCGTGTCAATGGAATTATCGACCAGATCATTGTATATATGGAAGCAATTGCCAAGAAGCAGAAGAAGG
  AGGAATCTTTATTCTTCATGGGAACAGAGGTGTTTACCATGACGATAAGCAACCAGCATTTAGAGCTGTT
  TATGAAGCACTGAGAAATTGTTCTTTTGAAGATGACAACATCCGTTCCTTATTAAAACCTTTAGAACTGG
  AACTACAAAAAACAGTGCATACATACTGTGGAAAAATTTACAAAATATTTATCAAACAACTAGCAAAAAG
  TGTAAGAGATCGTTATGCCAGATCACCAAAGGAAAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG213057 representing NM_001099650
  Red=Cloning site Green=Tags(s)
  
  MRRYLRVVVLCVACGFCSLLYAFSQLAVSLEEGTGGGGGKPQAAVASWLAGGGRGAVRGAGVAGPAAHPG
  VSDRYSLKIQPVEKMHLAVVACGERLEETMTMLKSAIIFSIKPLQFHIFAEDQLHHSFKGRLDNWSFLQT
  FNYTLYPITFPSENAAEWKKLFKPCASQRLFLPLILKEVDSLLYVDTDILFLRPVDDIWSLLKKFNSTQI
  AAMAPEHEEPRIGWYNRFARHPYYGKTGVNSGVMLMNMTRMRRKYFKNDMTTVRLQWGDILMPLLKKYKL
  NITWGDQDLLNIVFFHNPESLFVFPCQWNYRPDHCIYGSNCQEAEEGGIFILHGNRGVYHDDKQPAFRAV
  YEALRNCSFEDDNIRSLLKPLELELQKTVHTYCGKIYKIFIKQLAKSVRDRYARSPKEK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001099650
• ORF Size: 1227 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001099650.2
• RefSeq Size: 7416 bp
• RefSeq ORF: 1230 bp
• Locus ID: 283464
• UniProt ID: Q4G148
• Cytogenetics: 12q12
• Protein Families: Transmembrane
• Gene Summary: GXYLT1 is a xylosyltransferase (EC 2.4.2.-) that adds the first xylose to O-glucose-modified residues in the epidermal growth factor (EGF; MIM 131530) repeats of proteins such as NOTCH1 (MIM 190198) (Sethi et al., 2010 [PubMed 19940119]).[supplied by OMIM, Mar 2010]