MINDY2 (NM_001040450) Human Tagged ORF Clone

CAT#: RG213021

FAM63B (tGFP-tagged) - Human family with sequence similarity 63, member B (FAM63B), transcript variant 1

Product Images

Circular map for RG213021

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: MINDY2
• Synonyms: FAM63B
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG213021 representing NM_001040450
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAGCAGCCCCGAGAGCCTGCAGCCGCTAGAACACGGGGTGGCGGCCGGGCCAGCGTCAGGGACAG
  GTTCTTCGCAGGAAGGGCTACAGGAGACCAGGCTCGCCGCTGGTGATGGTCCTGGGGTATGGGCGGCGGA
  GACCAGCGGCGGGAATGGGCTGGGGGCGGCGGCCGCCAGGAGGAGCCTCCCGGACTCGGCTTCTCCCGCG
  GGCTCTCCTGAGGTTCCCGGACCCTGCAGCTCCTCCGCGGGTTTGGACTTGAAGGACAGTGGTTTGGAGA
  GTCCTGCTGCCGCCGAGGCGCCTCTGAGAGGGCAGTACAAGGTGACCGCCTCCCCGGAGACAGCCGTGGC
  CGGAGTGGGTCATGAGTTGGGTACCGCCGGAGACGCGGGAGCCCGCCCGGATCTCGCCGGCACCTGCCAA
  GCAGAACTGACCGCCGCCGGCTCCGAAGAGCCCAGCAGCGCCGGCGGCCTCAGCAGCAGTTGCAGCGACC
  CGAGCCCTCCTGGGGAATCTCCGAGCCTGGACTCTCTGGAGTCGTTCTCTAACCTGCATTCTTTTCCCAG
  TAGCTGCGAGTTCAATAGTGAGGAGGGAGCGGAGAACAGGGTCCCTGAGGAGGAGGAGGGCGCGGCGGTG
  TTGCCCGGGGCTGTTCCTCTGTGCAAGGAGGAGGAGGGGGAGGAGACCGCTCAGGTGCTGGCGGCCTCCA
  AGGAACGCTTCCCGGGACAATCTGTGTATCACATCAAGTGGATCCAGTGGAAGGAAGAGAACACACCCAT
  CATCACCCAGAATGAGAACGGACCCTGCCCCTTGCTGGCCATCCTCAATGTTTTGCTCCTGGCCTGGAAG
  GTGAAACTTCCACCGATGATGGAAATCATAACTGCTGAGCAGCTGATGGAATATTTAGGAGATTACATGC
  TTGATGCAAAGCCAAAAGAAATTTCAGAAATTCAACGTTTAAATTATGAACAGAATATGAGTGATGCCAT
  GGCAATTTTGCACAAACTACAGACAGGCCTGGATGTAAATGTAAGATTCACTGGTGTTCGAGTGTTTGAA
  TATACACCAGAATGCATAGTATTTGATCTTCTTGATATTCCTTTGTACCATGGGTGGTTAGTAGACCCTC
  AGATTGATGACATTGTAAAAGCTGTTGGTAACTGCAGCTACAACCAACTAGTGGAGAAGATCATCTCTTG
  TAAACAGTCAGACAATAGTGAGCTGGTTAGTGAAGGCTTTGTAGCTGAGCAGTTTCTAAATAACACAGCC
  ACTCAACTGACATACCATGGATTATGTGAACTAACTTCAACGGTTCAGGAAGGAGAACTTTGTGTGTTCT
  TTCGGAATAATCATTTTAGCACCATGACCAAATACAAGGGTCAACTGTATTTGTTGGTAACGGACCAGGG
  GTTTCTTACTGAAGAGAAAGTTGTTTGGGAAAGCCTACACAACGTAGATGGTGATGGAAATTTCTGTGAC
  TCAGAATTTCATCTTCGACCTCCTTCAGATCCTGAAACTGTATACAAAGGACAACAAGATCAGATAGATC
  AGGATTATCTTATGGCATTATCTCTACAACAAGAACAGCAGAGCCAAGAGATCAATTGGGAACAAATCCC
  GGAAGGAATCAGTGATTTGGAACTAGCAAAGAAACTCCAAGAGGAAGAGGACAGACGGGCTTCTCAATAC
  TATCAGGAACAGGAACAAGCAGCAGCTGCTGCTGCTGCTGCTTCTACACAGGCTCAGCAGGGCCAGCCAG
  CACAAGCCTCTCCATCAAGTGGAAGACAATCTGGGAATAGTGAACGTAAACGGAAGGAACCACGAGAAAA
  AGATAAAGAAAAAGAAAAGGAAAAAAATAGCTGTGTTATTTTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG213021 representing NM_001040450
  Red=Cloning site Green=Tags(s)
  
  MESSPESLQPLEHGVAAGPASGTGSSQEGLQETRLAAGDGPGVWAAETSGGNGLGAAAARRSLPDSASPA
  GSPEVPGPCSSSAGLDLKDSGLESPAAAEAPLRGQYKVTASPETAVAGVGHELGTAGDAGARPDLAGTCQ
  AELTAAGSEEPSSAGGLSSSCSDPSPPGESPSLDSLESFSNLHSFPSSCEFNSEEGAENRVPEEEEGAAV
  LPGAVPLCKEEEGEETAQVLAASKERFPGQSVYHIKWIQWKEENTPIITQNENGPCPLLAILNVLLLAWK
  VKLPPMMEIITAEQLMEYLGDYMLDAKPKEISEIQRLNYEQNMSDAMAILHKLQTGLDVNVRFTGVRVFE
  YTPECIVFDLLDIPLYHGWLVDPQIDDIVKAVGNCSYNQLVEKIISCKQSDNSELVSEGFVAEQFLNNTA
  TQLTYHGLCELTSTVQEGELCVFFRNNHFSTMTKYKGQLYLLVTDQGFLTEEKVVWESLHNVDGDGNFCD
  SEFHLRPPSDPETVYKGQQDQIDQDYLMALSLQQEQQSQEINWEQIPEGISDLELAKKLQEEEDRRASQY
  YQEQEQAAAAAAAASTQAQQGQPAQASPSSGRQSGNSERKRKEPREKDKEKEKEKNSCVIL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001040450
• ORF Size: 1863 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001040450.3
• RefSeq Size: 5013 bp
• RefSeq ORF: 1866 bp
• Locus ID: 54629
• UniProt ID: Q8NBR6
• Cytogenetics: 15q21.3-q22.1
• Protein Families: Druggable Genome
• Gene Summary: Hydrolase that can remove 'Lys-48'-linked conjugated ubiquitin from proteins (PubMed:27292798). Binds to polyubiquitin chains of different linkage types, including 'Lys-6', 'Lys-11', 'Lys-29', 'Lys-33', 'Lys-48' and 'Lys-63' (PubMed:28082312). May play a regulatory role at the level of protein turnover (PubMed:27292798).[UniProtKB/Swiss-Prot Function]