C19orf28 (MFSD12) (NM_021731) Human Tagged ORF Clone

CAT#: RG212962

MFSD12 (tGFP-tagged) - Human chromosome 19 open reading frame 28 (C19orf28), transcript variant 1

Product Images

Circular map for RG212962

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: C19orf28
• Synonyms: C19orf28; PP3501
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG212962 representing NM_021731
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCCCGGGACCCCCAGCGGCCGGAGCGGCGCCGTCCCCGCGGCCGCTGTCCCTGGTGGCGCGGCTGA
  GCTACGCCGTGGGCCACTTCCTCAACGACCTGTGCGCGTCCATGTGGTTCACCTACCTGCTGCTCTACCT
  GCACTCGGTGCGCGCCTACAGCTCCCGCGGCGCGGGGCTGCTGCTGCTGCTGGGCCAGGTGGCCGACGGG
  CTGTGCACACCGCTCGTGGGCTACGAGGCCGACCGCGCCGCCAGCTGCTGCGCCCGCTACGGCCCGCGCA
  AGGCCTGGCACCTGGTCGGCACCGTCTGCGTCCTGCTGTCCTTCCCCTTCATCTTCAGCCCCTGCCTGGG
  CTGTGGGGCGGCCACGCCCGAGTGGGCTGCCCTCCTCTACTACGGCCCGTTCATCGTGATCTTCCAGTTT
  GGCTGGGCCTCCACACAGATCTCCCACCTCAGCCTCATCCCGGAGCTCGTCACCAACGACCATGAGAAGG
  TGGAGCTCACGGCACTCAGGTATGCGTTCACCGTGGTGGCCAACATCACCGTCTACGGCGCCGCCTGGCT
  CCTGCTGCACCTGCAGGGCTCGTCGCGGGTGGAGCCCACCCAAGACATCAGCATCAGCGACCAGCTGGGG
  GGCCAGGACGTGCCCGTGTTCCGGAACCTGTCCCTGCTGGTGGTGGGTGTCGGCGCCGTGTTCTCACTGC
  TATTCCACCTGGGCACCCGGGAGAGGCGCCGGCCGCATGCGGAGGAGCCAGGCGAGCACACCCCCCTGTT
  GGCCCCTGCCACGGCCCAGCCCCTGCTGCTCTGGAAGCACTGGCTCCGGGAGCCGGCTTTCTACCAGGTG
  GGCATACTGTACATGACCACCAGGCTCATCGTGAACCTGTCCCAGACCTACATGGCCATGTACCTCACCT
  ACTCGCTCCACCTGCCCAAGAAGTTCATCGCGACCATTCCCCTGGTGATGTACCTCAGCGGCTTCTTGTC
  CTCCTTCCTCATGAAGCCCATCAACAAGTGCATTGGGAGGAACATGACCTACTTCTCAGGCCTCCTGGTG
  ATCCTGGCCTTTGCCGCCTGGGTGGCGCTGGCGGAGGGACTGGGTGTGGCCGTGTACGCAGCGGCTGTGC
  TGCTGGGTGCTGGCTGTGCCACCATCCTCGTCACCTCGCTGGCCATGACGGCCGACCTCATCGGTCCCCA
  CACGAACAGCGGAGCGTTCGTGTACGGCTCCATGAGCTTCTTGGATAAGGTGGCCAATGGGCTGGCAGTC
  ATGGCCATCCAGAGCCTGCACCCTTGCCCCTCAGAGCTCTGCTGCAGGGCCTGCGTGAGCTTTTACCACT
  GGGCGATGGTGGCTGTGACGGGCGGCGTGGGCGTGGCCGCTGCCCTGTGTCTCTGTAGCCTCCTGCTGTG
  GCCGACCCGCCTGCGACGCTCTTTTCTTGCCTGGAGAAGAGGGAGGGGAGAGGACAAGGGCCCTGGCTAC
  TCCTGGATTCCTACAGTCCTTGTCCAGCCTCCAAGACCCACAAGTCCCTTCCTCTGGGAAGCCCCCCTGG
  CCTGGAGGTGCACCAGGAAGAAGTGGTCTGGGGCTGGCACTAAGCCATGGCCCAGGGAAGACTGGGGGAC
  CCAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG212962 representing NM_021731
  Red=Cloning site Green=Tags(s)
  
  MGPGPPAAGAAPSPRPLSLVARLSYAVGHFLNDLCASMWFTYLLLYLHSVRAYSSRGAGLLLLLGQVADG
  LCTPLVGYEADRAASCCARYGPRKAWHLVGTVCVLLSFPFIFSPCLGCGAATPEWAALLYYGPFIVIFQF
  GWASTQISHLSLIPELVTNDHEKVELTALRYAFTVVANITVYGAAWLLLHLQGSSRVEPTQDISISDQLG
  GQDVPVFRNLSLLVVGVGAVFSLLFHLGTRERRRPHAEEPGEHTPLLAPATAQPLLLWKHWLREPAFYQV
  GILYMTTRLIVNLSQTYMAMYLTYSLHLPKKFIATIPLVMYLSGFLSSFLMKPINKCIGRNMTYFSGLLV
  ILAFAAWVALAEGLGVAVYAAAVLLGAGCATILVTSLAMTADLIGPHTNSGAFVYGSMSFLDKVANGLAV
  MAIQSLHPCPSELCCRACVSFYHWAMVAVTGGVGVAAALCLCSLLLWPTRLRRSFLAWRRGRGEDKGPGY
  SWIPTVLVQPPRPTSPFLWEAPLAWRCTRKKWSGAGTKPWPREDWGTH
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021731
• ORF Size: 1614 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021731.2, NP_068377.2
• RefSeq Size: 2284 bp
• RefSeq ORF: 1616 bp
• Locus ID: 126321
• Cytogenetics: 19p13.3
• Protein Families: Transmembrane
• Gene Summary: Transporter that mediates the import of cysteine into melanosomes, thereby regulating skin pigmentation (PubMed:33208952). In melanosomes, cysteine import is required both for normal levels of cystine, the oxidized dimer of cysteine, and provide cysteine for the production of the cysteinyldopas used in pheomelanin synthesis, thereby regulating skin pigmentation (PubMed:33208952). Also catalyzes import of cysteine into lysosomes in non-pigmented cells (PubMed:33208952).[UniProtKB/Swiss-Prot Function]