CPNE7 (NM_014427) Human Tagged ORF Clone

CAT#: RG212576

CPNE7 (tGFP-tagged) - Human copine VII (CPNE7), transcript variant 2

Product Images

Circular map for RG212576

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CPNE7
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG212576 representing NM_014427
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGCGGGCTCGGAGCGCGGGGCGGCGGCAACCCCCGGGGGTTTGCCCGCGCCCTGCGCCTCGAAGG
  TGGAGCTGCGGCTCAGCTGCCGGCACCTGCTGGACCGCGACCCGCTCACCAAGTCCGACCCCAGCGTGGC
  GTTGCTGCAGCAGGCGCAGGGCCAGTGGGTGCAGGTGGGCAGAACCGAGGTGGTCCGGAGCAGCCTGCAT
  CCCGTGTTCTCCAAGGTCTTCACGGTGGACTACTACTTCGAGGAGGTGCAGAGGCTGCGCTTTGAGGTGT
  ACGACACGCATGGGCCCAGCGGCTTCAGCTGTCAGGAGGACGATTTCCTGGGGGGCATGGAGTGCACCCT
  GGGGCAGCCAGCCCAAAAGTGGCTTCTGCAAGTCGTGATGAGAGTGTCTGTTGATGTGCTGGGCCCTGCT
  GGACACTGCGCTAAGCACTTCCTGTGCTGCACGGAATCCTCACACCTTGCCAGGACGGGTCCTTCTTTTT
  TATTGAGGTATGATGACCTCTGCCTCCCCTGGGCGACTGCTGGCGCCGTGAGGTGGTGGACGTGCAGGGG
  TGGCCACACGCAGGGATGGCAGATTGTGGCCCAGAAGAAGGTGACCCGCCCGCTGCTGCTCAAGTTTGGC
  AGGAACGCTGGCAAGTCCACCATCACGGTGATCGCCGAGGACATCTCGGGGAACAACGGCTACGTGGAGC
  TCTCCTTCCGGGCCAGGAAGCTGGACGACAAGGACCTCTTCAGCAAGTCCGACCCCTTCCTGGAGCTCTA
  CAGGGTCAACGACGACCAGGGCTTGCAGCTGGTGTACAGGACGGAGGTGGTGAAGAACAACCTGAACCCG
  GTGTGGGAGGCCTTCAAAGTCTCTCTGAGTTCCCTCTGCAGCTGCGAGGAGACAAGGCCTCTAAAGTGCC
  TGGTCTGGGATTACGACTCTCGAGGAAAGCACGACTTCATCGGAGAATTCTCTACCACCTTCGAGGAGAT
  GCAGAAGGCCTTTGAGGAGGGGCAGGCCCAGTGGGACTGTGTGAACCCCAAATACAAGCAGAAGAGACGC
  AGTTATAAGAACTCAGGAGTGGTCGTCCTGGCTGACCTCAAGTTCCACAGGGTGTACTCCTTCCTGGACT
  ATATCATGGGCGGCTGCCAGATCCACTTCACCGTGGCCATTGACTTCACCGCCTCCAATGGAGACCCGCG
  GAACAGCTGCTCCCTGCACTACATCAACCCCTACCAGCCGAACGAGTACCTGAAGGCACTGGTGTCCGTG
  GGCGAGATCTGCCAGGACTATGACAGTGACAAGAGGTTTTCCGCTTTGGGGTTTGGAGCCCGGATCCCTC
  CCAAGTATGAGGTGTCCCATGACTTTGCCATCAATTTCAACCCTGAGGACGATGAGTGTGAAGGCATCCA
  GGGCGTGGTGGAGGCCTACCAGAACTGCCTGCCCAGGGTCCAGCTCTACGGCCCCACCAACGTGGCGCCC
  ATCATCTCCAAGGTGGCACGCGTGGCGGCGGCCGAGGAGAGCACCGGGAAAGCCTCTCAATACTACATCC
  TGCTGATCCTGACGGACGGCGTGGTGACCGACATGGCCGACACACGGGAGGCCATTGTGCGTGCCTCACG
  CCTGCCCATGTCCATCATCATCGTGGGCGTGGGCAACGCCGACTTCACCGACATGCAGGTCCTGGACGGC
  GACGACGGCGTCCTGCGCTCCCCACGGGGTGAGCCCGCGCTCCGGGACATCGTACAGTTCGTGCCCTTCC
  GGGAGCTCAAGAACGCATCCCCTGCGGCGCTGGCCAAGTGCGTGCTGGCCGAGGTCCCGAAGCAGGTGGT
  GGAGTACTACAGCCACAGAGGCCTGCCCCCGAGAAGCCTGGGCGTCCCTGCCGGAGAGGCCAGCCCAGGC
  TGCACACCG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG212576 representing NM_014427
  Red=Cloning site Green=Tags(s)
  
  MSAGSERGAAATPGGLPAPCASKVELRLSCRHLLDRDPLTKSDPSVALLQQAQGQWVQVGRTEVVRSSLH
  PVFSKVFTVDYYFEEVQRLRFEVYDTHGPSGFSCQEDDFLGGMECTLGQPAQKWLLQVVMRVSVDVLGPA
  GHCAKHFLCCTESSHLARTGPSFLLRYDDLCLPWATAGAVRWWTCRGGHTQGWQIVAQKKVTRPLLLKFG
  RNAGKSTITVIAEDISGNNGYVELSFRARKLDDKDLFSKSDPFLELYRVNDDQGLQLVYRTEVVKNNLNP
  VWEAFKVSLSSLCSCEETRPLKCLVWDYDSRGKHDFIGEFSTTFEEMQKAFEEGQAQWDCVNPKYKQKRR
  SYKNSGVVVLADLKFHRVYSFLDYIMGGCQIHFTVAIDFTASNGDPRNSCSLHYINPYQPNEYLKALVSV
  GEICQDYDSDKRFSALGFGARIPPKYEVSHDFAINFNPEDDECEGIQGVVEAYQNCLPRVQLYGPTNVAP
  IISKVARVAAAEESTGKASQYYILLILTDGVVTDMADTREAIVRASRLPMSIIIVGVGNADFTDMQVLDG
  DDGVLRSPRGEPALRDIVQFVPFRELKNASPAALAKCVLAEVPKQVVEYYSHRGLPPRSLGVPAGEASPG
  CTP
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014427
• ORF Size: 1899 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014427.3, NP_055242.1
• RefSeq Size: 2657 bp
• RefSeq ORF: 1902 bp
• Locus ID: 27132
• UniProt ID: Q9UBL6
• Cytogenetics: 16q24.3
• Gene Summary: This gene encodes a member of the copine family, which is composed of calcium-dependent membrane-binding proteins. The gene product contains two N-terminal C2 domains and one von Willebrand factor A domain. The encoded protein may be involved in membrane trafficking. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2008]