IKBKE (NM_014002) Human Tagged ORF Clone

CAT#: RG212481

IKBKE (tGFP-tagged) - Human inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon (IKBKE), transcript variant 1

Product Images

Circular map for RG212481

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: IKBKE
• Synonyms: IKK-E; IKK-i; IKKE; IKKI
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG212481 representing NM_014002
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGAGCACAGCCAATTACCTGTGGCACACAGATGACCTGCTGGGGCAGGGGGCCACTGCCAGTGTGT
  ACAAGGCCCGCAACAAGAAATCCGGAGAGCTGGTTGCTGTGAAGGTCTTCAACACTACCAGCTACCTGCG
  GCCCCGCGAGGTGCAGGTGAGGGAGTTTGAGGTCCTGCGGAAGCTGAACCACCAGAACATCGTCAAGCTC
  TTTGCGGTGGAGGAGACGGGCGGAAGCCGGCAGAAGGTACTGGTGATGGAGTACTGCTCCAGTGGGAGCC
  TGCTGAGTGTGCTGGAGAGCCCTGAGAATGCCTTTGGGCTGCCTGAGGATGAGTTCCTGGTGGTGCTGCG
  CTGTGTGGTGGCCGGCATGAACCACCTGCGGGAGAACGGCATTGTGCATCGCGACATCAAGCCGGGGAAC
  ATCATGCGCCTCGTAGGGGAGGAGGGGCAGAGCATCTACAAGCTGACAGACTTCGGCGCTGCCCGGGAGC
  TGGATGATGATGAGAAGTTCGTCTCGGTCTATGGGACTGAGGAGTACCTGCATCCCGACATGTATGAGCG
  GGCGGTGCTTCGAAAGCCCCAGCAAAAAGCGTTCGGGGTGACTGTGGATCTCTGGAGCATTGGAGTGACC
  TTGTACCATGCAGCCACTGGCAGCCTGCCCTTCATCCCCTTTGGTGGGCCACGGCGGAACAAGGAGATCA
  TGTACCGGATCACCACGGAGAAGCCGGCTGGGGCCATTGCAGGTGCCCAGAGGCGGGAGAACGGGCCCCT
  GGAGTGGAGCTACACCCTCCCCATCACCTGCCAGCTGTCACTGGGGCTGCAGAGCCAGCTGGTGCCCATC
  CTGGCCAACATCCTGGAGGTGGAGCAGGCCAAGTGCTGGGGCTTCGACCAGTTCTTTGCGGAGACCAGTG
  ACATCCTGCAGCGAGTTGTCGTCCATGTCTTCTCCCTGTCCCAGGCAGTCCTGCACCACATCTATATCCA
  TGCCCACAACACGATAGCCATTTTCCAGGAGGCCGTGCACAAGCAGACCAGTGTGGCCCCCCGACACCAG
  GAGTACCTCTTTGAGGGTCACCTCTGTGTCCTCGAGCCCAGCGTCTCAGCACAGCACATCGCCCACACGA
  CGGCAAGCAGCCCCCTGACCCTCTTCAGCACAGCCATCCCTAAGGGGCTGGCCTTCAGGGACCCTGCTCT
  GGACGTCCCCAAGTTCGTCCCCAAAGTGGACCTGCAGGCGGATTACAACACTGCCAAGGGCGTGTTGGGC
  GCCGGCTACCAGGCCCTGCGGCTGGCACGGGCCCTGCTGGATGGGCAGGAGCTAATGTTTCGGGGGCTGC
  ACTGGGTCATGGAGGTGCTCCAGGCCACATGCAGACGGACTCTGGAAGTGGCAAGGACATCCCTCCTCTA
  CCTCAGCAGCAGCCTGGGAACTGAGAGGTTCAGCAGCGTGGCTGGAACGCCTGAGATCCAGGAACTGAAG
  GCGGCTGCAGAACTGAGGTCCAGGCTGCGGACTCTAGCGGAGGTCCTCTCCAGATGCTCCCAAAATATCA
  CGGAGACCCAGGAGAGCCTGAGCAGCCTGAACCGGGAGCTGGTGAAGAGCCGGGATCAGGTACATGAGGA
  CAGAAGCATCCAGCAGATTCAGTGCTGTTTGGACAAGATGAACTTCATCTACAAACAGTTCAAGAAGTCT
  AGGATGAGGCCAGGGCTTGGCTACAACGAGGAGCAGATTCACAAGCTGGATAAGGTGAATTTCAGTCATT
  TAGCCAAAAGACTCCTGCAGGTGTTCCAGGAGGAGTGCGTGCAGAAGTATCAAGCGTCCTTAGTCACACA
  CGGCAAGAGGATGAGGGTGGTGCACGAGACCAGGAACCACCTGCGCCTGGTTGGCTGTTCTGTGGCTGCC
  TGTAACACAGAAGCCCAGGGGGTCCAGGAGAGTCTCAGCAAGCTCCTGGAAGAGCTATCTCACCAGCTCC
  TTCAGGACCGAGCAAAGGGGGCTCAGGCCTCGCCGCCTCCCATAGCTCCTTACCCCAGCCCTACACGAAA
  GGACCTGCTTCTCCACATGCAAGAGCTCTGCGAGGGGATGAAGCTGCTGGCATCTGACCTCCTGGACAAC
  AACCGCATCATCGAACGGCTAAATAGAGTCCCAGCACTTCCTGATGTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG212481 representing NM_014002
  Red=Cloning site Green=Tags(s)
  
  MQSTANYLWHTDDLLGQGATASVYKARNKKSGELVAVKVFNTTSYLRPREVQVREFEVLRKLNHQNIVKL
  FAVEETGGSRQKVLVMEYCSSGSLLSVLESPENAFGLPEDEFLVVLRCVVAGMNHLRENGIVHRDIKPGN
  IMRLVGEEGQSIYKLTDFGAARELDDDEKFVSVYGTEEYLHPDMYERAVLRKPQQKAFGVTVDLWSIGVT
  LYHAATGSLPFIPFGGPRRNKEIMYRITTEKPAGAIAGAQRRENGPLEWSYTLPITCQLSLGLQSQLVPI
  LANILEVEQAKCWGFDQFFAETSDILQRVVVHVFSLSQAVLHHIYIHAHNTIAIFQEAVHKQTSVAPRHQ
  EYLFEGHLCVLEPSVSAQHIAHTTASSPLTLFSTAIPKGLAFRDPALDVPKFVPKVDLQADYNTAKGVLG
  AGYQALRLARALLDGQELMFRGLHWVMEVLQATCRRTLEVARTSLLYLSSSLGTERFSSVAGTPEIQELK
  AAAELRSRLRTLAEVLSRCSQNITETQESLSSLNRELVKSRDQVHEDRSIQQIQCCLDKMNFIYKQFKKS
  RMRPGLGYNEEQIHKLDKVNFSHLAKRLLQVFQEECVQKYQASLVTHGKRMRVVHETRNHLRLVGCSVAA
  CNTEAQGVQESLSKLLEELSHQLLQDRAKGAQASPPPIAPYPSPTRKDLLLHMQELCEGMKLLASDLLDN
  NRIIERLNRVPALPDV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014002
• ORF Size: 2148 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014002.4
• RefSeq Size: 3263 bp
• RefSeq ORF: 2151 bp
• Locus ID: 9641
• UniProt ID: Q14164
• Cytogenetics: 1q32.1
• Domains: pkinase
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Cytosolic DNA-sensing pathway, RIG-I-like receptor signaling pathway, Toll-like receptor signaling pathway
• Gene Summary: IKBKE is a noncanonical I-kappa-B (see MIM 164008) kinase (IKK) that is essential for regulating antiviral signaling pathways. IKBKE has also been identified as a breast cancer (MIM 114480) oncogene and is amplified and overexpressed in over 30% of breast carcinomas and breast cancer cell lines (Hutti et al., 2009 [PubMed 19481526]).[supplied by OMIM, Oct 2009]