AMMECR1 (NM_015365) Human Tagged ORF Clone

CAT#: RG212378

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  • TrueORF®

AMMECR1 (GFP-tagged) - Human Alport syndrome, mental retardation, midface hypoplasia and elliptocytosis chromosomal region gene 1 (AMMECR1), transcript variant 1



  "NM_015365" in other vectors (4)

USD 460.00


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Size
    • 10 ug


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Specifications

Product Data
Product Name AMMECR1 (NM_015365) Human Tagged ORF Clone
Type Human Tagged ORF Clone
Tag TurboGFP
Symbol AMMECR1
Synonyms AMMERC1; MFHIEN
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Cell Selection Neomycin
Sequence Data
>RG212378 representing NM_015365
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCGGGTTGCTGCGGGGTGAAGAAGCAGAAACTGTCCAGTTCGCCCCCCTCTGGCTCGGGTGGCG
GTGGTGGCGCCTCCTCCTCCTCCCACTGCAGCGGAGAGAGCCAGTGCCGAGCTGGGGAGCTGGGACTAGG
AGGCGCCGGTACGCGGCTCAACGGGCTGGGAGGTCTAACCGGAGGAGGTAGCGGCAGCGGCTGTACCCTC
TCTCCCCCCCAGGGCTGCGGCGGCGGCGGCGGGGGGATCGCCCTGTCGCCACCTCCGAGCTGCGGAGTGG
GGACCCTACTTTCTACCCCGGCCGCCGCCACCTCTTCCTCACCCTCCTCATCGTCCGCCGCCTCGTCCTC
ATCGCCGGGCTCCCGGAAGATGGTGGTGTCAGCAGAGATGTGCTGCTTTTGCTTCGATGTGCTCTACTGT
CACCTGTATGGATACCAGCAGCCCCGGACCCCCCGATTCACCAACGAGCCCTACCCACTGTTTGTAACAT
GGAAGATTGGTCGAGACAAAAGATTACGTGGATGCATAGGTACTTTTTCTGCCATGAATTTGCATTCAGG
ACTCAGGGAGTACACACTTACCAGTGCCCTTAAAGATAGCCGTTTTCCCCCAATGACAAGGGATGAGCTG
CCACGGCTTTTCTGCTCAGTGTCTCTGCTCACTAACTTTGAAGATGTCTGTGATTATTTGGACTGGGAGG
TGGGTGTACATGGCATTAGAATAGAATTCATCAATGAAAAAGGATCAAAACGCACCGCCACCTACCTACC
GGAGGTTGCAAAGGAGCAAGGATGGGACCATATACAGACCATAGACTCCTTATTGAGGAAAGGAGGATAC
AAAGCTCCGATTACTAATGAATTCAGGAAAACCATAAAACTGACCAGGTATCGTAGTGAAAAGATGACCC
TGAGCTATGCTGAATACCTTGCTCATCGCCAGCATCATCATTTCCAAAATGGCATTGGGCATCCCCTTCC
GCCATACAACCATTATTCC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG212378 representing NM_015365
Red=Cloning site Green=Tags(s)

MAAGCCGVKKQKLSSSPPSGSGGGGGASSSSHCSGESQCRAGELGLGGAGTRLNGLGGLTGGGSGSGCTL
SPPQGCGGGGGGIALSPPPSCGVGTLLSTPAAATSSSPSSSSAASSSSPGSRKMVVSAEMCCFCFDVLYC
HLYGYQQPRTPRFTNEPYPLFVTWKIGRDKRLRGCIGTFSAMNLHSGLREYTLTSALKDSRFPPMTRDEL
PRLFCSVSLLTNFEDVCDYLDWEVGVHGIRIEFINEKGSKRTATYLPEVAKEQGWDHIQTIDSLLRKGGY
KAPITNEFRKTIKLTRYRSEKMTLSYAEYLAHRQHHHFQNGIGHPLPPYNHYS

TRTRPLE - GFP Tag - V
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
Tag TurboGFP
ACCN NM_015365
ORF Size 999 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_015365.2, NP_056180.1
RefSeq Size 5431 bp
RefSeq ORF 1002 bp
Locus ID 9949
Cytogenetics Xq23
Protein Families Druggable Genome
Gene Summary The exact function of this gene is not known, however, submicroscopic deletion of the X chromosome including this gene, COL4A5, and FACL4 genes, result in a contiguous gene deletion syndrome, the AMME complex (Alport syndrome, mental retardation, midface hypoplasia, and elliptocytosis). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2010]

Citations (1)

Other Versions

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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