MEGF9 (NM_001080497) Human Tagged ORF Clone

CAT#: RG212354

MEGF9 (tGFP-tagged) - Human multiple EGF-like-domains 9 (MEGF9)

Product Images

Circular map for RG212354

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: MEGF9
• Synonyms: EGFL5
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG212354 representing NM_001080497
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGAGCCTGCCGAGCCTGGGCGGCCTCGCCCTGTTGTGCTGCGCCGCCGCCGCCGCCGCCGCCGCCG
  TCGCCTCAGCCGCCTCGGCGGGGAATGTCACCGGTGGCGGCGGGGCCGCGGGGCAGGTGGACGCGTCGCC
  GGGCCCCGGGTTGCGGGGCGAGCCCAGCCACCCCTTCCCTAGGGCGACGGCTCCCACGGCCCAGGCCCCG
  AGGACCGGGCCCCCGCGCGCCACCGTCCACCGACCCCTGGCTGCGACTTCTCCAGCCCAGTCCCCGGAGA
  CCACCCCTCTTTGGGCGACTGCTGGACCCTCTTCCACCACCTTTCAGGCGCCGCTCGGCCCCTCGCCGAC
  CACCCCTCCGGCGGCGGAACGCACTTCGACCACCTCTCAGGCGCCGACCAGACCCGCGCCGACCACCCTT
  TCGACGACCACTGGCCCGGCGCCGACCACCCCTGTAGCGACCACCGTACCGGCGCCCACGACTCCCCGGA
  CCCCGACCCCCGATCTCCCCAGCAGCAGCAACAGCAGCGTCCTCCCCACCCCACCTGCCACCGAGGCCCC
  CTCTTCGCCTCCTCCAGGGCATCAGTGGCCTGTGGCAAAGATGCCCCAAAAGTATCTAGGAAAATATGCC
  TGTGAGAGTAACCTGAAAAGCAAGTACTTGCCTCTTACACAGCCAGTCATGAATCTTAGAGTCTCAGAAG
  CTGTTAAAACGGAGTATGTATGTAACTGCTCTGTGGTTGGAAGCCTGAATGTGAATCGCTGCAACCAGAC
  CACAGGGCAGTGTGAGTGTCGGCCAGGTTATCAGGGGCTTCACTGTGAAACCTGCAAAGAGGGCTTTTAC
  CTAAATTACACTTCTGGGCTCTGTCAGCCATGTGACTGTAGTCCACATGGAGCTCTCAGCATACCGTGCA
  ACAGTTCTGGGAAATGCCAGTGCAAAGTGGGTGTCATTGGCTCTATATGTGACCGATGCCAAGATGGATA
  TTATGGCTTTAGTAAGAATGGCTGCTTGCCCTGCCAATGCAATAATCGGTCTGCCAGTTGCGATGCCCTC
  ACAGGTGCTTGTTTAAACTGCCAGGAAAATAGCAAAGGAAATCACTGTGAAGAATGTAAAGAAGGATTTT
  ATCAGAGTCCTGATGCCACTAAAGAATGTCTTCGCTGCCCTTGTTCAGCAGTGACATCTACAGGCAGCTG
  CTCTATAAAATCGAGTGAATTGGAACCTGAATGTGACCAGTGTAAAGATGGTTACATAGGCCCGAACTGC
  AATAAATGTGAAAATGGCTATTACAATTTTGACAGCATCTGTAGAAAGTGCCAATGTCACGGCCATGTGG
  ACCCAGTTAAAACTCCAAAGATTTGTAAGCCCGAGAGTGGTGAGTGCATCAACTGCCTCCATAACACCAC
  TGGGTTTTGGTGTGAGAACTGCCTAGAAGGTTATGTTCACGACCTCGAGGGAAATTGCATCAAGAAAGAA
  GTTATTCTTCCAACACCTGAAGGTTCTACCATTTTGGTTTCCAATGCCTCTTTGACCACATCAGTGCCCA
  CCCCTGTTATAAATAGTACTTTTACCCCTACAACCCTGCAGACTATCTTTTCAGTAAGCACTTCTGAAAA
  CAGCACTTCAGCTTTAGCTGATGTATCATGGACCCAATTTAACATCATCATTTTGACAGTCATCATCATT
  GTTGTGGTGCTGCTAATGGGATTTGTGGGGGCTGTATATATGTACCGCGAGTACCAAAACCGGAAACTCA
  ATGCCCCCTTTTGGACCATCGAGCTGAAAGAAGACAATATCAGTTTCAGCAGCTACCATGACAGCATTCC
  CAATGCAGATGTTTCGGGATTGTTGGAAGATGATGGCAATGAAGTGGCTCCCAATGGGCAGCTGACCCTG
  ACGACGCCCATACATAACTACAAAGCC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG212354 representing NM_001080497
  Red=Cloning site Green=Tags(s)
  
  MRSLPSLGGLALLCCAAAAAAAAVASAASAGNVTGGGGAAGQVDASPGPGLRGEPSHPFPRATAPTAQAP
  RTGPPRATVHRPLAATSPAQSPETTPLWATAGPSSTTFQAPLGPSPTTPPAAERTSTTSQAPTRPAPTTL
  STTTGPAPTTPVATTVPAPTTPRTPTPDLPSSSNSSVLPTPPATEAPSSPPPGHQWPVAKMPQKYLGKYA
  CESNLKSKYLPLTQPVMNLRVSEAVKTEYVCNCSVVGSLNVNRCNQTTGQCECRPGYQGLHCETCKEGFY
  LNYTSGLCQPCDCSPHGALSIPCNSSGKCQCKVGVIGSICDRCQDGYYGFSKNGCLPCQCNNRSASCDAL
  TGACLNCQENSKGNHCEECKEGFYQSPDATKECLRCPCSAVTSTGSCSIKSSELEPECDQCKDGYIGPNC
  NKCENGYYNFDSICRKCQCHGHVDPVKTPKICKPESGECINCLHNTTGFWCENCLEGYVHDLEGNCIKKE
  VILPTPEGSTILVSNASLTTSVPTPVINSTFTPTTLQTIFSVSTSENSTSALADVSWTQFNIIILTVIII
  VVVLLMGFVGAVYMYREYQNRKLNAPFWTIELKEDNISFSSYHDSIPNADVSGLLEDDGNEVAPNGQLTL
  TTPIHNYKA
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001080497
• ORF Size: 1917 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001080497.1, NP_001073966.1
• RefSeq Size: 6297 bp
• RefSeq ORF: 1809 bp
• Locus ID: 1955
• UniProt ID: Q9H1U4
• Cytogenetics: 9q33.2