MLH3 (NM_014381) Human Tagged ORF Clone

CAT#: RG212150

MLH3 (tGFP-tagged) - Human mutL homolog 3 (E. coli) (MLH3), transcript variant 2

Product Images

Circular map for RG212150

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: MLH3
• Synonyms: HNPCC7
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG212150 representing NM_014381
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCAAGTGCTTGTCAGTTGAAGTACAAGCCAAATTGCGTTCTGGTTTGGCCATAAGCTCCTTGGGCC
  AATGTGTTGAGGAACTTGCCCTCAACAGTATTGATGCTGAAGCAAAATGTGTGGCTGTCAGGGTGAATAT
  GGAAACCTTCCAAGTTCAAGTGATAGACAATGGATTTGGGATGGGGAGTGATGATGTAGAGAAAGTGGGA
  AATCGTTATTTCACCAGTAAATGCCACTCGGTACAGGACTTGGAGAATCCAAGGTTTTATGGTTTCCGAG
  GAGAGGCCTTGGCAAATATTGCTGACATGGCCAGTGCTGTGGAAATTTCGTCCAAGAAAAACAGGACAAT
  GAAAACTTTTGTGAAACTGTTTCAGAGTGGAAAAGCCCTGAAAGCTTGTGAAGCTGATGTGACTAGAGCA
  AGCGCTGGGACTACTGTAACAGTGTATAACCTATTTTACCAGCTTCCTGTAAGGAGGAAATGCATGGACC
  CTAGACTGGAGTTTGAGAAGGTTAGGCAGAGAATAGAAGCTCTCTCACTCATGCACCCTTCCATTTCTTT
  CTCTTTGAGAAATGATGTTTCTGGTTCCATGGTTCTTCAGCTCCCTAAAACCAAAGACGTATGTTCCCGA
  TTTTGTCAAATTTATGGATTGGGAAAGTCCCAAAAGCTAAGAGAAATAAGTTTTAAATATAAAGAGTTTG
  AGCTTAGTGGCTATATCAGCTCTGAAGCACATTACAACAAGAATATGCAGTTTTTGTTTGTGAACAAAAG
  ACTAGTTTTAAGGACAAAGCTACATAAACTCATTGACTTTTTATTAAGGAAAGAAAGTATTATATGCAAG
  CCAAAGAATGGTCCCACCAGTAGGCAAATGAATTCAAGTCTTCGGCACCGGTCTACCCCAGAACTCTATG
  GCATATATGTAATTAATGTGCAGTGCCAATTCTGTGAGTATGATGTGTGCATGGAGCCAGCCAAAACTCT
  GATTGAATTTCAGAACTGGGACACTCTCTTGTTTTGCATTCAGGAAGGAGTGAAAATGTTTTTAAAGCAA
  GAAAAATTATTTGTGGAATTATCAGGTGAGGATATTAAGGAATTTAGTGAAGATAATGGTTTTAGTTTAT
  TTGATGCTACTCTTCAGAAGCGTGTGACTTCCGATGAGAGGAGCAATTTCCAGGAAGCATGTAATAATAT
  TTTAGATTCCTATGAGATGTTTAATTTGCAGTCAAAAGCTGTGAAAAGAAAAACTACTGCAGAAAACGTA
  AACACACAGAGTTCTAGGGATTCAGAAGCTACCAGAAAAAATACAAATGATGCATTTTTGTACATTTATG
  AATCAGGTGGTCCAGGCCATAGCAAAATGACAGAGCCATCTTTACAAAACAAAGACAGCTCTTGCTCAGA
  ATCAAAGATGTTAGAACAAGAGACAATTGTAGCATCAGAAGCTGGAGAAAATGAGAAACATAAAAAATCT
  TTCCTGGAACATAGCTCTTTAGAAAATCCGTGTGGAACCAGTTTAGAAATGTTTTTAAGCCCTTTTCAGA
  CACCATGTCACTTTGAGGAGAGTGGGCAGGATCTAGAAATATGGAAAGAAAGTACTACTGTTAATGGCAT
  GGCTGCCAACATCTTGAAAAATAATAGAATTCAGAATCAACCAAAGAGATTTAAAGATGCTACTGAAGTG
  GGATGCCAGCCTCTGCCTTTTGCAACAACATTATGGGGAGTACATAGTGCTCAGACAGAGAAAGAGAAAA
  AAAAAGAATCTAGCAATTGTGGAAGAAGAAATGTTTTTAGTTATGGGCGAGTTAAATTATGTTCCACTGG
  CTTTATAACTCATGTAGTACAAAATGAAAAAACTAAATCAACTGAAACAGAACATTCATTTAAAAATTAT
  GTTAGACCTGGTCCCACACGTGCCCAAGAAACATTTGGAAATAGAACACGTCATTCAGTTGAAACTCCAG
  ACATCAAAGATTTAGCCAGCACTTTAAGTAAAGAATCTGGTCAATTGCCCAACAAAAAAAATTGCAGAAC
  GAATATAAGTTATGGGCTAGAGAATGAACCTACAGCAACTTATACAATGTTTTCTGCTTTTCAGGAAGGT
  AGCAAAAAATCACAAACAGATTGCATATTATCTGATACATCCCCCTCTTTCCCCTGGTATAGACACGTTT
  CCAATGATAGTAGGAAAACAGATAAATTAATTGGTTTCTCCAAACCAATCGTCCGTAAGAAGCTAAGCTT
  GAGTTCACAGCTAGGATCTTTAGAGAAGTTTAAGAGGCAATATGGGAAGGTTGAAAATCCTCTGGATACA
  GAAGTAGAGGAAAGTAATGGAGTCACTACCAATCTCAGTCTTCAAGTTGAACCTGACATTCTGCTGAAGG
  ACAAGAACCGCTTAGAGAACTCTGATGTTTGTAAAATCACTACTATGGAGCATAGTGATTCAGATAGTAG
  TTGTCAACCAGCAAGCCACATCCTTGACTCAGAGAAGTTTCCATTCTCCAAGGATGAAGATTGTTTAGAA
  CAACAGATGCTTAGTTTGAGAGAAAGTCCTATGACCCTGAAGGAGTTATCTCTCTTTAATAGAAAACCTT
  TGGACCTTGAGAAGTCATCTGAATCACTAGCCTCTAAATTATCCAGACTGAAGGGTTCCGAAAGAGAAAC
  TCAAACAATGGGGATGATGAGTCGTTTTAATGAACTTCCAAATTCAGATTCCAGTAGGAAAGACAGCAAG
  TTGTGCAGTGTGTTAACACAAGATTTTTGTATGTTATTTAACAACAAGCATGAAAAAACAGAGAATGGTG
  TCATCCCAACATCAGATTCTGCCACACAGGATAATTCCTTTAATAAAAATAGTAAAACACATTCTAACAG
  CAATACAACAGAGAACTGTGTGATATCAGAAACTCCTTTGGTATTGCCCTATAATAATTCTAAAGTTACC
  GGTAAAGATTCAGATGTTCTTATCAGAGCCTCAGAACAACAGATAGGAAGTCTTGACTCTCCCAGTGGAA
  TGTTAATGAATCCGGTAGAAGATGCCACAGGTGACCAAAATGGAATTTGTTTTCAGAGTGAGGAATCTAA
  AGCAAGAGCTTGTTCTGAAACTGAAGAGTCAAACACGTGTTGTTCAGATTGGCAGCGGCATTTCGATGTA
  GCCCTGGGAAGAATGGTTTATGTCAACAAAATGACTGGACTCAGCACATTCATTGCCCCAACTGAGGACA
  TTCAGGCTGCTTGTACTAAAGACCTGACAACTGTGGCTGTGGATGTTGTACTTGAGAATGGGTCTCAGTA
  CAGGTGTCAACCTTTTAGAAGCGACCTTGTTCTTCCTTTCCTTCCGAGAGCTCGAGCAGAGAGGACTGTG
  ATGAGACAGGATAACAGAGATACTGTGGATGATACTGTTAGTAGCGAATCGCTTCAGTCTTTGTTCTCAG
  AATGGGACAATCCAGTATTTGCCCGTTATCCAGAGGTTGCTGTTGATGTAAGCAGTGGCCAGGCTGAGAG
  CTTAGCAGTTAAAATTCACAACATCTTGTATCCCTATCGTTTCACCAAAGGAATGATTCATTCAATGCAG
  GTTCTCCAGCAAGTAGATAACAAGTTTATTGCCTGTTTGATGAGCACTAAGACTGAAGAGAATGGCGAGG
  CAGATTCCTACGAGAAGCAACAGGCACAAGGCTCTGGTCGGAAAAAATTACTGTCTTCTACTCTAATTCC
  TCCGCTAGAGATAACAGTGACAGAGGAACAAAGGAGACTCTTATGGTGTTACCACAAAAATCTGGAAGAT
  CTGGGCCTTGAATTTGTATTTCCAGACACTAGTGATTCTCTGGTCCTTGTGGGAAAAGTACCACTATGTT
  TTGTGGAAAGAGAAGCCAATGAACTTCGGAGAGGAAGATCTACTGTGACCAAGAGTATTGTGGAGGAATT
  TATCCGAGAACAACTGGAGCTACTCCAGACCACCGGAGGCATCCAAGGGACATTGCCACTGACTGTCCAG
  AAGGTGTTGGCATCCCAAGCCTGCCATGGGGCCATTAAGTTTAATGATGGCCTGAGCTTACAGGAAAGTT
  GCCGCCTTATTGAAGCTCTGTCCTCATGCCAGCTGCCATTCCAGTGTGCTCACGGGAGACCTTCTATGCT
  GCCGTTAGCTGACATAGACCACTTGGAACAGGAAAAACAGATTAAACCCAACCTCACTAAACTTCGCAAA
  ATGGCCCAGGCCTGGCGTCTCTTTGGAAAAGCAGAGTGTGATACAAGGCAGAGCCTGCAGCAATCCATGC
  CTCCCTGTGAGCCACCA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG212150 representing NM_014381
  Red=Cloning site Green=Tags(s)
  
  MIKCLSVEVQAKLRSGLAISSLGQCVEELALNSIDAEAKCVAVRVNMETFQVQVIDNGFGMGSDDVEKVG
  NRYFTSKCHSVQDLENPRFYGFRGEALANIADMASAVEISSKKNRTMKTFVKLFQSGKALKACEADVTRA
  SAGTTVTVYNLFYQLPVRRKCMDPRLEFEKVRQRIEALSLMHPSISFSLRNDVSGSMVLQLPKTKDVCSR
  FCQIYGLGKSQKLREISFKYKEFELSGYISSEAHYNKNMQFLFVNKRLVLRTKLHKLIDFLLRKESIICK
  PKNGPTSRQMNSSLRHRSTPELYGIYVINVQCQFCEYDVCMEPAKTLIEFQNWDTLLFCIQEGVKMFLKQ
  EKLFVELSGEDIKEFSEDNGFSLFDATLQKRVTSDERSNFQEACNNILDSYEMFNLQSKAVKRKTTAENV
  NTQSSRDSEATRKNTNDAFLYIYESGGPGHSKMTEPSLQNKDSSCSESKMLEQETIVASEAGENEKHKKS
  FLEHSSLENPCGTSLEMFLSPFQTPCHFEESGQDLEIWKESTTVNGMAANILKNNRIQNQPKRFKDATEV
  GCQPLPFATTLWGVHSAQTEKEKKKESSNCGRRNVFSYGRVKLCSTGFITHVVQNEKTKSTETEHSFKNY
  VRPGPTRAQETFGNRTRHSVETPDIKDLASTLSKESGQLPNKKNCRTNISYGLENEPTATYTMFSAFQEG
  SKKSQTDCILSDTSPSFPWYRHVSNDSRKTDKLIGFSKPIVRKKLSLSSQLGSLEKFKRQYGKVENPLDT
  EVEESNGVTTNLSLQVEPDILLKDKNRLENSDVCKITTMEHSDSDSSCQPASHILDSEKFPFSKDEDCLE
  QQMLSLRESPMTLKELSLFNRKPLDLEKSSESLASKLSRLKGSERETQTMGMMSRFNELPNSDSSRKDSK
  LCSVLTQDFCMLFNNKHEKTENGVIPTSDSATQDNSFNKNSKTHSNSNTTENCVISETPLVLPYNNSKVT
  GKDSDVLIRASEQQIGSLDSPSGMLMNPVEDATGDQNGICFQSEESKARACSETEESNTCCSDWQRHFDV
  ALGRMVYVNKMTGLSTFIAPTEDIQAACTKDLTTVAVDVVLENGSQYRCQPFRSDLVLPFLPRARAERTV
  MRQDNRDTVDDTVSSESLQSLFSEWDNPVFARYPEVAVDVSSGQAESLAVKIHNILYPYRFTKGMIHSMQ
  VLQQVDNKFIACLMSTKTEENGEADSYEKQQAQGSGRKKLLSSTLIPPLEITVTEEQRRLLWCYHKNLED
  LGLEFVFPDTSDSLVLVGKVPLCFVEREANELRRGRSTVTKSIVEEFIREQLELLQTTGGIQGTLPLTVQ
  KVLASQACHGAIKFNDGLSLQESCRLIEALSSCQLPFQCAHGRPSMLPLADIDHLEQEKQIKPNLTKLRK
  MAQAWRLFGKAECDTRQSLQQSMPPCEPP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014381
• ORF Size: 4287 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014381.3
• RefSeq Size: 4895 bp
• RefSeq ORF: 4290 bp
• Locus ID: 27030
• Cytogenetics: 14q24.3
• Domains: HATPase_c
• Protein Families: Druggable Genome
• Protein Pathways: Mismatch repair
• Gene Summary: This gene is a member of the MutL-homolog (MLH) family of DNA mismatch repair (MMR) genes. MLH genes are implicated in maintaining genomic integrity during DNA replication and after meiotic recombination. The protein encoded by this gene functions as a heterodimer with other family members. Somatic mutations in this gene frequently occur in tumors exhibiting microsatellite instability, and germline mutations have been linked to hereditary nonpolyposis colorectal cancer type 7 (HNPCC7). Several alternatively spliced transcript variants have been identified, but the full-length nature of only two transcript variants has been determined. [provided by RefSeq, Jul 2008]