HNRPH2 (HNRNPH2) (NM_001032393) Human Tagged ORF Clone

CAT#: RG212052

HNRNPH2 (tGFP-tagged) - Human heterogeneous nuclear ribonucleoprotein H2 (H') (HNRNPH2), transcript variant 2

Product Images

Circular map for RG212052

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: HNRPH2
• Synonyms: FTP3; hnRNPH'; HNRPH'; HNRPH2; MRXSB; NRPH2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG212052 representing NM_001032393
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGCTGAGCACGGAAGGCAGGGAGGGGTTCGTGGTGAAGGTCAGGGGCCTACCCTGGTCCTGCTCAG
  CCGATGAAGTGATGCGCTTCTTCTCTGATTGCAAGATCCAAAATGGCACATCAGGTATTCGTTTCATCTA
  CACCAGAGAAGGCAGACCAAGTGGTGAAGCATTTGTTGAACTTGAATCTGAAGAGGAAGTGAAATTGGCT
  TTGAAGAAGGACAGAGAAACCATGGGACACAGATACGTTGAAGTATTCAAGTCTAACAGTGTTGAAATGG
  ATTGGGTGTTGAAGCATACAGGTCCGAATAGCCCTGATACTGCCAACGATGGCTTCGTCCGGCTTAGAGG
  ACTCCCATTTGGCTGTAGCAAGGAAGAGATTGTTCAGTTCTTTTCAGGGTTGGAAATTGTGCCAAATGGG
  ATGACACTGCCAGTGGACTTTCAGGGGCGAAGCACAGGGGAAGCCTTTGTGCAGTTTGCTTCACAGGAGA
  TAGCTGAGAAGGCCTTAAAGAAACACAAGGAAAGAATAGGGCACAGGTACATTGAGATCTTCAAGAGTAG
  CCGAGCTGAAGTTCGAACCCACTATGATCCCCCTCGAAAGCTCATGGCTATGCAGCGGCCAGGTCCCTAT
  GATAGGCCGGGGGCTGGCAGAGGGTATAATAGCATTGGCAGAGGAGCTGGGTTTGAAAGGATGAGGCGTG
  GTGCCTATGGTGGAGGGTATGGAGGCTATGATGACTATGGTGGCTATAATGATGGATATGGCTTTGGGTC
  TGATAGATTTGGAAGAGACCTCAATTACTGTTTTTCAGGAATGTCTGATCATAGATACGGAGATGGTGGG
  TCCAGTTTCCAGAGCACCACAGGGCACTGTGTACACATGAGGGGGTTACCTTACAGAGCCACTGAGAATG
  ATATTTATAATTTCTTCTCACCTCTTAATCCCATGAGAGTACATATTGAAATTGGACCCGATGGCAGAGT
  TACCGGTGAGGCAGATGTTGAATTTGCTACTCATGAAGATGCTGTGGCAGCTATGGCAAAAGACAAAGCT
  AATATGCAACACAGATATGTGGAGCTCTTCTTAAATTCTACTGCAGGAACAAGTGGGGGTGCTTACGATC
  ACAGCTATGTAGAACTTTTTTTGAATTCTACAGCAGGGGCAAGTGGTGGCGCTTATGGTAGCCAAATGAT
  GGGAGGGATGGGCTTATCCAACCAGTCTAGTTATGGAGGTCCTGCTAGCCAGCAGCTGAGTGGTGGTTAT
  GGAGGTGGTTATGGTGGTCAGAGCAGTATGAGTGGATATGACCAAGTTCTGCAGGAAAACTCCAGTGACT
  ATCAGTCAAACCTTGCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG212052 representing NM_001032393
  Red=Cloning site Green=Tags(s)
  
  MMLSTEGREGFVVKVRGLPWSCSADEVMRFFSDCKIQNGTSGIRFIYTREGRPSGEAFVELESEEEVKLA
  LKKDRETMGHRYVEVFKSNSVEMDWVLKHTGPNSPDTANDGFVRLRGLPFGCSKEEIVQFFSGLEIVPNG
  MTLPVDFQGRSTGEAFVQFASQEIAEKALKKHKERIGHRYIEIFKSSRAEVRTHYDPPRKLMAMQRPGPY
  DRPGAGRGYNSIGRGAGFERMRRGAYGGGYGGYDDYGGYNDGYGFGSDRFGRDLNYCFSGMSDHRYGDGG
  SSFQSTTGHCVHMRGLPYRATENDIYNFFSPLNPMRVHIEIGPDGRVTGEADVEFATHEDAVAAMAKDKA
  NMQHRYVELFLNSTAGTSGGAYDHSYVELFLNSTAGASGGAYGSQMMGGMGLSNQSSYGGPASQQLSGGY
  GGGYGGQSSMSGYDQVLQENSSDYQSNLA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001032393
• ORF Size: 1347 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001032393.2, NP_001027565.1
• RefSeq Size: 2315 bp
• RefSeq ORF: 1350 bp
• Locus ID: 3188
• UniProt ID: P55795
• Cytogenetics: Xq22.1
• Gene Summary: This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that binds to RNAs. It is very similar to the family member HNRPH1. This gene is thought to be involved in Fabray disease and X-linked agammaglobulinemia phenotype. Alternative splicing results in multiple transcript variants encoding the same protein. Read-through transcription between this locus and the ribosomal protein L36a gene has been observed. [provided by RefSeq, Jan 2011]