EPB41 (NM_004437) Human Tagged ORF Clone

CAT#: RG212040

EPB41 (tGFP-tagged) - Human erythrocyte membrane protein band 4.1 (elliptocytosis 1, RH-linked) (EPB41), transcript variant 6

Product Images

Circular map for RG212040

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: EPB41
• Synonyms: 4.1R; EL1; HE
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG212040 representing NM_004437
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACTGCAAGGTTTCTTTGTTGGATGACACAGTTTATGAATGTGTTGTGGAGAAACATGCTAAGGGAC
  AAGATTTGCTTAAACGAGTATGTGAGCATCTCAATCTTTTGGAAGAAGACTATTTTGGTCTAGCCATTTG
  GGATAACGCAACCTCTAAGACATGGCTGGATTCCGCCAAAGAAATAAAAAAGCAGGTTCGTGGTGTCCCT
  TGGAATTTTACATTTAATGTAAAGTTTTATCCACCTGACCCAGCACAGTTAACAGAAGACATAACAAGAT
  ATTATTTATGTCTTCAGCTTCGGCAGGACATAGTTGCAGGACGTCTGCCCTGTTCCTTTGCAACCTTAGC
  ATTATTAGGTTCTTACACCATCCAGTCTGAACTGGGAGACTACGACCCAGAACTCCATGGCGTGGATTAT
  GTTAGTGATTTTAAACTGGCCCCGAATCAGACCAAGGAACTTGAAGAGAAGGTCATGGAACTGCATAAGT
  CATACAGGTCCATGACTCCAGCTCAGGCTGACTTGGAGTTTCTTGAGAATGCCAAAAAGTTGTCTATGTA
  TGGAGTTGATCTTCATAAAGCAAAGGACTTGGAAGGAGTAGATATCATCCTAGGTGTCTGCTCTAGTGGC
  CTTCTGGTTTACAAAGATAAGCTGAGAATTAACCGCTTCCCTTGGCCCAAAGTGCTGAAGATTTCTTATA
  AACGTAGTAGCTTTTTCATCAAGATTCGGCCTGGAGAGCAAGAGCAGTATGAAAGTACCATCGGATTCAA
  ACTTCCCAGTTACCGAGCAGCTAAGAAATTATGGAAAGTCTGTGTAGAACATCACACGTTTTTCAGATTG
  ACATCTACAGACACCATTCCCAAAAGCAAATTTCTTGCGCTAGGATCCAAATTTCGATACAGTGGCCGGA
  CTCAAGCTCAGACCAGGCAAGCTAGTGCTCTAATTGACAGGCCTGCCCCACACTTCGAGCGTACAGCAAG
  TAAACGGGCGTCCCGGAGCCTCGATGGAGCAGCAGCTGTCGATTCGGCAGACCGAAGTCCTCGGCCCACT
  TCTGCACCTGCCATTACTCAGGGTCAGGTTGCAGAAGGTGGCGTCCTAGATGCCTCTGCTAAAAAAACAG
  TGGTCCCTAAAGCACAGAAGGAAACAGTGAAGGCTGAAGTGAAAAAGGAAGACGAGCCACCTGAGCAAGC
  TGAGCCAGAGCCCACAGAAGCATGGAAGAAAAAGAGAGAAAGACTAGATGGTGAAAACATTTATATCAGA
  CATAGCAATTTAATGTTGGAGGATTTAGACAAGAGTCAAGAGGAGATCAAAAAACATCATGCCAGCATCA
  GTGAGCTGAAAAAGAACTTCATGGAGTCTGTACCAGAACCACGGCCTAGTGAATGGGATAAACGCTTATC
  CACTCACTCACCCTTCCGAACTCTTAACATCAATGGGCAAATCCCCACAGGAGAAGGACCTCCCCTGGTG
  AAGACACAAACTGTCACCATCTCAGATAATGCCAATGCTGTGAAAAGTGAAATCCCAACCAAAGACGTCC
  CTATTGTCCACACTGAGACCAAGACCATCACTTATGAGGCTGCCCAGACTGTAAAAGGTGGGATTTCAGA
  GACACGTATTGAAAAGAGAATTGTGATCACAGGAGATGCTGATATTGACCATGATCAGGTCCTTGTACAA
  GCCATCAAGGAGGCAAAGGAGCAGCACCCAGACATGTCAGTGACCAAGGTGGTCGTCCACCAGGAGACCG
  AGATTGCTGATGAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG212040 representing NM_004437
  Red=Cloning site Green=Tags(s)
  
  MHCKVSLLDDTVYECVVEKHAKGQDLLKRVCEHLNLLEEDYFGLAIWDNATSKTWLDSAKEIKKQVRGVP
  WNFTFNVKFYPPDPAQLTEDITRYYLCLQLRQDIVAGRLPCSFATLALLGSYTIQSELGDYDPELHGVDY
  VSDFKLAPNQTKELEEKVMELHKSYRSMTPAQADLEFLENAKKLSMYGVDLHKAKDLEGVDIILGVCSSG
  LLVYKDKLRINRFPWPKVLKISYKRSSFFIKIRPGEQEQYESTIGFKLPSYRAAKKLWKVCVEHHTFFRL
  TSTDTIPKSKFLALGSKFRYSGRTQAQTRQASALIDRPAPHFERTASKRASRSLDGAAAVDSADRSPRPT
  SAPAITQGQVAEGGVLDASAKKTVVPKAQKETVKAEVKKEDEPPEQAEPEPTEAWKKKRERLDGENIYIR
  HSNLMLEDLDKSQEEIKKHHASISELKKNFMESVPEPRPSEWDKRLSTHSPFRTLNINGQIPTGEGPPLV
  KTQTVTISDNANAVKSEIPTKDVPIVHTETKTITYEAAQTVKGGISETRIEKRIVITGDADIDHDQVLVQ
  AIKEAKEQHPDMSVTKVVVHQETEIADE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004437
• ORF Size: 1764 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004437.4
• RefSeq Size: 5823 bp
• RefSeq ORF: 1767 bp
• Locus ID: 2035
• UniProt ID: P11171
• Cytogenetics: 1p35.3
• Domains: B41, SAB
• Protein Families: Druggable Genome
• Protein Pathways: Tight junction
• Gene Summary: The protein encoded by this gene, together with spectrin and actin, constitute the red cell membrane cytoskeletal network. This complex plays a critical role in erythrocyte shape and deformability. Mutations in this gene are associated with type 1 elliptocytosis (EL1). Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Oct 2009]