INPP4A (NM_004027) Human Tagged ORF Clone

CAT#: RG211900

INPP4A (tGFP-tagged) - Human inositol polyphosphate-4-phosphatase, type I, 107kDa (INPP4A), transcript variant a

Product Images

Circular map for RG211900

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: INPP4A
• Synonyms: INPP4; TVAS1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG211900 representing NM_004027
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAGCAAGAGAGCACAGCCCTCGCCATGGTGCCAGGGCCCGTGCAATGCAGCGGGCTTCCACCATCG
  ACGTGGCGGCCGACATGCTGGGCCTCTCTCTGGCAGGAAATATACAAGACCCAGATGAGCCCATTTTAGA
  ATTTAGCTTAGCTTGCAGTGAGCTGCATACTCCATCGCTAGATCGAAAGCCAAATAGTTTTGTTGCGGTG
  AGTGTCACCACCCCTCCTCAGGCATTCTGGACGAAGCATGCACAGACGGAGATCATTGAGGGAACCAACA
  ATCCTATATTTCTAAGCAGTATTGCCTTCTTTCAAGACTCTCTTATCAATCAGATGACACAAGTCAAACT
  CTCCGTGTATGATGTCAAAGATAGATCTCAGGGAACAATGTATTTACTGGGCTCTGGAACGTTCATTGTC
  AAAGATCTGCTCCAGGACAGGCATCATAGGTTGCATTTAACACTAAGGTCTGCAGAGAGTGACCGTGTAG
  GTAACATCACCGTGATTGGCTGGCAGATGGAGGAGAAGTCAGACCAACGGCCCCCTGTGACCCGGTCTGT
  GGACACTGTCAATGGGAGGATGGTTCTTCCTGTCGATGAGAGCTTGACGGAGGCGTTAGGAATCCGATCC
  AAATACGCTTCATTGCGAAAGGACACTTTGCTGAAATCGGTGTTCGGTGGTGCCATCTGCCGCATGTACC
  GGTTTCCAACCACTGATGGTAACCATTTGCGGATCCTGGAGCAGATGGCAGAGAGCGTGCTCTCCCTGCA
  CGTGCCCCGGCAGTTCGTGAAGCTCCTACTAGAGGAAGATGCAGCCAGAGTGTGTGAGCTGGAGGAGCTG
  GGAGAGCTGTCCCCTTGCTGGGAGAGCCTCCGGCGCCAAATTGTCACCCAGTACCAGACCATCATCCTCA
  CATACCAGGAGAACCTGACCGACCTCCATCAGTACAGAGGGCCCTCGTTTAAAGCAAGCAGTTTGAAAGC
  AGATAAAAAGTTAGAATTTGTTCCCACAAACTTGCACATACAAAGGATGAGAGTTCAAGACGATGGAGGA
  TCAGATCAGAACTACGACATCGTCACCATTGGGGCGCCAGCAGCACACTGCCAAGGTTTTAAGTCAGGAG
  GTCTCCGCAAAAAGCTGCACAAATTTGAAGAGACCAAGAAACATTTTGAGGAGTGTTGTACATCATCTGG
  CTGCCAGTCCATAATCTACATACCCCAGGATGTTGTCAGAGCCAAGGAGATCATCGCCCAGATCAACACC
  CTGAAAACCCAAGTGAGTTACTACGCAGAGCGGCTGTCAAGGGCAGCCAAGGACAGGTCTGCCACTGGCC
  TTGAGAGGACACTCGCCATCTTGGCAGACAAGACACGGCAGCTGGTCACGGTCTGCGACTGCAAGCTCCT
  GGCCAACTCCATCCATGGGCTGAACGCTGCACGGCCTGACTACATTGCCTCCAAGGCCTCTCCCACTTCG
  ACTGAGGAGGAGCAGGTGATGCTTAGAAATGACCAGGACACCCTCATGGCCCGGTGGACAGGGAGAAACA
  GCCGATCTTCCCTGCAGGTGGACTGGCACGAGGAGGAGTGGGAGAAAGTGTGGCTGAACGTGGACAAGAG
  CCTAGAGTGCATCATTCAGCGTGTGGACAAGCTGCTGCAGAAGGAGCGGCTGCATGGCGAGGGCTGTGAG
  GATGTCTTCCCCTGTGCAGGCAGCTGCACCAGCAAGAAAGATTGCAGTCCCCCTCCTGAAGAGTCCAGCC
  CAGGTGAATGGAGTGAGGCCCTTTACCCGCTGCTGACCACTCTCACCGACTGCGTGGCCATGATGAGTGA
  CAAGGCCAAGAAGGCCATGGTATTCCTGCTCATGCAGGACAGCGCGCCCACCATAGCCACCTACCTGAGC
  CTGCAGTACCGCCGTGACGTGGTCTTCTGCCAGACGCTGACCGCCCTCATCTGCGGCTTCATCATTAAGC
  TGAGGAACTGCCTGCATGACGACGGCTTCCTGCGCCAGCTCTACACCATCGGGCTGCTGGCCCAGTTCGA
  GAGCCTGCTGAGCACCTACGGGGAGGAGCTGGCAATGCTGGAGGACATGAGCCTTGGGATCATGGACTTG
  AGGAACGTGACCTTCAAAGTCACTCAGGCCACTTCCAGCGCCTCCGCAGACATGCTGCCCGTCATCACAG
  GAAATCGCGACGGGTTTAACGTGCGGGTCCCTCTGCCGGGCCCGCTGTTTGACGCCTTGCCCCGGGAGAT
  CCAGAGTGGCATGCTGCTGCGAGTGCAGCCCGTCCTCTTCAACGTGGGCATCAATGAGCAGCAGACACTG
  GCCGAGAGGTTTGGCGATACGTCTTTACAAGAAGTCATCAACGTGGAGAGTTTGGTGCGGTTAAATTCCT
  ACTTTGAGCAGTTTAAGGAAGTTTTGCCTGAGGATTGCCTGCCTCGGTCTCGCAGTCAGACGTGCCTGCC
  AGAGCTGCTGCGGTTTCTGGGTCAGAACGTGCATGCCCGGAAGAATAAGAACGTCGACATTCTCTGGCAA
  GCTGCTGAGATCTGCCGCCGCCTTAATGGGGTCCGGTTCACCAGCTGCAAGAGCGCTAAGGACCGTACAG
  CCATGTCGGTGACACTGGAGCAGTGCCTGATCCTGCAACACGAGCATGGCATGGCCCCGCAGGTCTTCAC
  CCAGGCCCTGGAGTGCATGCGCAGTGAGGGTTGTCGAAGAGAAAATACAATGAAGAATGTTGGAAGTCGC
  AAATATGCATTTAATTCCCTGCAGCTGAAGGCTTTCCCCAAGCATTACAGGCCTCCCGAAGGGACTTACG
  GAAAAGTTGAAACG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG211900 representing NM_004027
  Red=Cloning site Green=Tags(s)
  
  MTAREHSPRHGARARAMQRASTIDVAADMLGLSLAGNIQDPDEPILEFSLACSELHTPSLDRKPNSFVAV
  SVTTPPQAFWTKHAQTEIIEGTNNPIFLSSIAFFQDSLINQMTQVKLSVYDVKDRSQGTMYLLGSGTFIV
  KDLLQDRHHRLHLTLRSAESDRVGNITVIGWQMEEKSDQRPPVTRSVDTVNGRMVLPVDESLTEALGIRS
  KYASLRKDTLLKSVFGGAICRMYRFPTTDGNHLRILEQMAESVLSLHVPRQFVKLLLEEDAARVCELEEL
  GELSPCWESLRRQIVTQYQTIILTYQENLTDLHQYRGPSFKASSLKADKKLEFVPTNLHIQRMRVQDDGG
  SDQNYDIVTIGAPAAHCQGFKSGGLRKKLHKFEETKKHFEECCTSSGCQSIIYIPQDVVRAKEIIAQINT
  LKTQVSYYAERLSRAAKDRSATGLERTLAILADKTRQLVTVCDCKLLANSIHGLNAARPDYIASKASPTS
  TEEEQVMLRNDQDTLMARWTGRNSRSSLQVDWHEEEWEKVWLNVDKSLECIIQRVDKLLQKERLHGEGCE
  DVFPCAGSCTSKKDCSPPPEESSPGEWSEALYPLLTTLTDCVAMMSDKAKKAMVFLLMQDSAPTIATYLS
  LQYRRDVVFCQTLTALICGFIIKLRNCLHDDGFLRQLYTIGLLAQFESLLSTYGEELAMLEDMSLGIMDL
  RNVTFKVTQATSSASADMLPVITGNRDGFNVRVPLPGPLFDALPREIQSGMLLRVQPVLFNVGINEQQTL
  AERFGDTSLQEVINVESLVRLNSYFEQFKEVLPEDCLPRSRSQTCLPELLRFLGQNVHARKNKNVDILWQ
  AAEICRRLNGVRFTSCKSAKDRTAMSVTLEQCLILQHEHGMAPQVFTQALECMRSEGCRRENTMKNVGSR
  KYAFNSLQLKAFPKHYRPPEGTYGKVET
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004027
• ORF Size: 2814 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004027.3
• RefSeq Size: 3217 bp
• RefSeq ORF: 2817 bp
• Locus ID: 3631
• UniProt ID: Q96PE3
• Cytogenetics: 2q11.2
• Protein Families: Transmembrane
• Protein Pathways: Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system
• Gene Summary: This gene encodes an Mg++ independent enzyme that hydrolyzes the 4-position phosphate from the inositol ring of phosphatidylinositol 3,4-bisphosphate, inositol 1,3,4-trisphosphate, and inositol 3,4-bisphosphate. Multiple transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Aug 2008]