Rho GTPase activating protein 24 (ARHGAP24) (NM_001042669) Human Tagged ORF Clone

CAT#: RG211886

ARHGAP24 (tGFP-tagged) - Human Rho GTPase activating protein 24 (ARHGAP24), transcript variant 3

Product Images

Circular map for RG211886

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Rho GTPase activating protein 24
• Synonyms: FILGAP; p73; p73RhoGAP; RC-GAP72; RCGAP72
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG211886 representing NM_001042669
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAGCAAATCATGAAAGCTACCTCCTCATGGCAAGCACCCAGAATGATATGGAAGACTGGGTGAAGT
  CAATCCGCCGAGTCATATGGGGACCTTTCGGAGGAGGCATTTTTGGACAGAAACTGGAGGATACTGTTCG
  TTATGAGAAGAGATATGGGAACCGTCTGGCTCCGATGTTGGTGGAGCAGTGCGTGGACTTTATCCGACAA
  AGGGGGCTGAAAGAAGAGGGTCTCTTTCGACTGCCAGGCCAGGCTAATCTTGTTAAGGAGCTCCAAGATG
  CCTTTGACTGTGGGGAGAAGCCATCATTTGACAGCAACACAGATGTACACACGGTGGCATCACTTCTTAA
  GCTGTACCTCCGAGAACTTCCAGAACCAGTTATTCCTTATGCGAAGTATGAAGATTTTTTGTCATGTGCC
  AAACTGCTCAGCAAGGAAGAGGAAGCAGGTGTTAAGGAATTAGCAAAGCAGGTGAAGAGTTTGCCAGTGG
  TAAATTACAACCTCCTCAAGTATATTTGCAGATTCTTGGATGAAGTACAGTCCTACTCGGGAGTTAACAA
  AATGAGTGTGCAGAACTTGGCAACGGTCTTTGGTCCTAATATCCTGCGCCCCAAAGTGGAAGATCCTTTG
  ACTATCATGGAGGGCACTGTGGTGGTCCAGCAGTTGATGTCAGTGATGATTAGCAAACATGATTGCCTCT
  TTCCCAAAGATGCAGAACTACAAAGCAAGCCCCAAGATGGAGTGAGCAACAACAATGAAATTCAGAAGAA
  AGCCACCATGGGGCAGTTACAGAACAAGGAGAACAATAACACCAAGGACAGCCCTAGTAGGCAGTGCTCC
  TGGGACAAGTCTGAGTCACCCCAGAGAAGCAGCATGAACAATGGATCCCCCACAGCTCTATCAGGCAGCA
  AAACCAACAGCCCAAAGAACAGTGTTCACAAGCTAGATGTGTCTAGAAGCCCCCCTCTCATGGTCAAAAA
  GAACCCAGCCTTTAATAAGGGTAGTGGGATAGTTACCAATGGGTCCTTCAGCAGCAGTAATGCAGAAGGT
  CTTGAGAAAACCCAAACCACCCCCAATGGGAGCCTACAGGCCAGAAGGAGCTCTTCACTGAAGGTATCTG
  GTACCAAAATGGGCACGCACAGTGTACAGAATGGAACGGTGCGCATGGGCATTTTGAACAGCGACACACT
  CGGGAACCCCACAAATGTTCGAAACATGAGCTGGCTGCCAAATGGCTATGTGACCCTGAGGGATAACAAG
  CAGAAAGAACAAGCTGGAGAGTTAGGCCAGCACAACAGACTGTCCACCTATGATAATGTCCATCAACAGT
  TCTCCATGATGAACCTTGATGACAAGCAGAGCATTGACAGTGCTACCTGGTCCACTTCCTCCTGTGAAAT
  CTCCCTCCCTGAGAACTCCAACTCCTGTCGCTCTTCTACCACCACCTGCCCAGAGCAAGACTTTTTTGGG
  GGGAACTTTGAGGACCCTGTTTTGGATGGGCCCCCGCAGGACGACCTTTCCCACCCCAGGGACTATGAAA
  GCAAAAGTGACCACAGGAGTGTGGGAGGTCGAAGTAGTCGTGCCACCAGTAGCAGTGACAACAGTGAGAC
  ATTTGTGGGCAACAGCAGCAGCAACCACAGTGCACTGCACAGTTTAGTTTCCAGCCTGAAACAGGAAATG
  ACCAAACAGAAGATAGAGTATGAGTCCAGGATAAAGAGCTTAGAACAGCGAAACTTGACTTTGGAAACAG
  AAATGATGAGCCTCCATGATGAACTGGATCAGGAGAGGAAAAAGTTCACAATGATAGAAATAAAAATGCG
  AAATGCCGAGCGAGCAAAAGAAGATGCCGAGAAAAGAAATGACATGCTACAGAAAGAAATGGAGCAGTTT
  TTTTCCACGTTTGGAGAACTGACAGTGGAACCCAGGAGAACCGAGAGAGGAAACACAATATGGATTCAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG211886 representing NM_001042669
  Red=Cloning site Green=Tags(s)
  
  MTANHESYLLMASTQNDMEDWVKSIRRVIWGPFGGGIFGQKLEDTVRYEKRYGNRLAPMLVEQCVDFIRQ
  RGLKEEGLFRLPGQANLVKELQDAFDCGEKPSFDSNTDVHTVASLLKLYLRELPEPVIPYAKYEDFLSCA
  KLLSKEEEAGVKELAKQVKSLPVVNYNLLKYICRFLDEVQSYSGVNKMSVQNLATVFGPNILRPKVEDPL
  TIMEGTVVVQQLMSVMISKHDCLFPKDAELQSKPQDGVSNNNEIQKKATMGQLQNKENNNTKDSPSRQCS
  WDKSESPQRSSMNNGSPTALSGSKTNSPKNSVHKLDVSRSPPLMVKKNPAFNKGSGIVTNGSFSSSNAEG
  LEKTQTTPNGSLQARRSSSLKVSGTKMGTHSVQNGTVRMGILNSDTLGNPTNVRNMSWLPNGYVTLRDNK
  QKEQAGELGQHNRLSTYDNVHQQFSMMNLDDKQSIDSATWSTSSCEISLPENSNSCRSSTTTCPEQDFFG
  GNFEDPVLDGPPQDDLSHPRDYESKSDHRSVGGRSSRATSSSDNSETFVGNSSSNHSALHSLVSSLKQEM
  TKQKIEYESRIKSLEQRNLTLETEMMSLHDELDQERKKFTMIEIKMRNAERAKEDAEKRNDMLQKEMEQF
  FSTFGELTVEPRRTERGNTIWIQ
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001042669
• ORF Size: 1959 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001042669.2
• RefSeq Size: 4195 bp
• RefSeq ORF: 1962 bp
• Locus ID: 83478
• UniProt ID: Q8N264
• Cytogenetics: 4q21.23-q21.3
• Gene Summary: This gene encodes a Rho-GTPase activating protein, which is specific for the small GTPase family member Rac. Binding of the encoded protein by filamin A targets it to sites of membrane protrusion, where it antognizes Rac. This results in suppression of lamellae formation and promotion of retraction to regulate cell polarity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016]