Glucuronic Acid Epimerase (GLCE) (NM_015554) Human Tagged ORF Clone

CAT#: RG211866

GLCE (tGFP-tagged) - Human glucuronic acid epimerase (GLCE)

Product Images

Circular map for RG211866

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Glucuronic Acid Epimerase
• Synonyms: HSEPI
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG211866 representing NM_015554
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGTTGCTTGGCAGCTCGGGTCAACTATAAGACTTTGATTATTATCTGCGCACTCTTCACTTTGGTCA
  CAGTACTTTTGTGGAATAAGTGTTCCAGTGACAAAGCAATCCAGTTTCCACGGCGTTCGAGTAGTGGCTT
  CAGAGTGGATGGGTTTGAAAAAAGAGCAGCAGCATCTGAGAGTAACAACTATATGAACCACGTGGCCAAA
  CAACAGTCTGAGGAAGCATTCCCTCAGGAACAGCAGAAAGCACCCCCTGTTGTTGGGGGCTTCAATAGCA
  ATGTGGGAAGTAAGGTGTTAGGGCTCAAATATGAAGAAATTGACTGTCTCATAAATGATGAACACACAAT
  TAAAGGGAGACGAGAGGGGAACGAAGTCTTTCTTCCATTCACTTGGGTTGAGAAATATTTTGATGTTTAT
  GGAAAGGTGGTTCAGTATGATGGCTATGATCGGTTTGAATTCTCTCATAGCTATTCCAAAGTCTATGCAC
  AGAGAGCCCCCTATCACCCCGATGGTGTGTTTATGTCTTTTGAAGGCTACAATGTGGAAGTCCGAGACAG
  AGTCAAGTGCATAAGTGGGGTTGAAGGTGTGCCATTATCTACACAATGGGGACCTCAAGGCTATTTCTAT
  CCAATCCAGATTGCACAGTATGGATTAAGTCATTACAGCAAGAATCTAACTGAGAAACCTCCTCACATAG
  AGGTATATGAAACAGCAGAAGACAGAGACAAAAACAAGCCTAATGACTGGACTGTGCCAAAGGGCTGCTT
  TATGGCGAATGTGGCTGATAAGTCTAGATTCACCAATGTCAAACAGTTTATTGCACCAGAAACCAGTGAA
  GGTGTATCCTTGCAACTGGGAAACACAAAAGATTTTATTATTTCATTTGACCTCAAGTTCTTGACAAATG
  GAAGTGTGTCCGTGGTTCTAGAGACCACAGAAAAGAATCAGCTCTTCACTATACATTATGTCTCAAATGC
  TCAGCTAATTGCTTTTAAAGAAAGAGATATATACTATGGCATTGGGCCCAGAACTTCATGGAGCACAGTT
  ACCAGGGACCTGGTCACTGACCTCAGGAAAGGAGTGGGTCTTTCAAACACAAAAGCTGTCAAGCCAACCA
  AAATAATGCCCAAGAAGGTGGTTAGGTTGATTGCAAAAGGTAAGGGATTCCTCGACAACATTACCATCTC
  TACCACAGCCCACATGGCTGCATTTTTTGCTGCTAGTGATTGGCTAGTAAGGAACCAGGATGAGAAAGGT
  GGCTGGCCAATTATGGTGACCCGTAAGTTAGGGGAAGGGTTCAAGTCTTTAGAGCCAGGATGGTATTCTG
  CCATGGCCCAAGGGCAAGCCATTTCTACATTAGTCAGGGCCTATCTGTTAACAAAAGACCATATATTCCT
  CAATTCAGCTTTAAGGGCAACAGCCCCTTATAAGTTTCTATCTGAGCAGCATGGAGTTAAAGCTGTGTTT
  ATGAATAAACATGACTGGTATGAAGAATATCCAACCACACCTAGCTCTTTTGTTTTAAATGGCTTTATGT
  ATTCTTTAATTGGGCTGTATGACTTAAAAGAAACTGCAGGGGAAAAACTCGGAAAAGAAGCAAGGTCCTT
  GTATGAGCGTGGCATGGAATCTCTTAAAGCCATGCTGCCCTTGTATGACACTGGCTCAGGAACCATCTAT
  GACCTCCGTCACTTCATGCTTGGCATCGCTCCTAACCTGGCTCGCTGGGACTATCATACCACCCACATCA
  ATCAGTTGCAGCTACTCAGTACCATTGATGAGTCCCCAGTCTTCAAAGAATTTGTCAAGAGGTGGAAAAG
  CTACCTTAAAGGCAGCAGGGCAAAGCACAAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG211866 representing NM_015554
  Red=Cloning site Green=Tags(s)
  
  MRCLAARVNYKTLIIICALFTLVTVLLWNKCSSDKAIQFPRRSSSGFRVDGFEKRAAASESNNYMNHVAK
  QQSEEAFPQEQQKAPPVVGGFNSNVGSKVLGLKYEEIDCLINDEHTIKGRREGNEVFLPFTWVEKYFDVY
  GKVVQYDGYDRFEFSHSYSKVYAQRAPYHPDGVFMSFEGYNVEVRDRVKCISGVEGVPLSTQWGPQGYFY
  PIQIAQYGLSHYSKNLTEKPPHIEVYETAEDRDKNKPNDWTVPKGCFMANVADKSRFTNVKQFIAPETSE
  GVSLQLGNTKDFIISFDLKFLTNGSVSVVLETTEKNQLFTIHYVSNAQLIAFKERDIYYGIGPRTSWSTV
  TRDLVTDLRKGVGLSNTKAVKPTKIMPKKVVRLIAKGKGFLDNITISTTAHMAAFFAASDWLVRNQDEKG
  GWPIMVTRKLGEGFKSLEPGWYSAMAQGQAISTLVRAYLLTKDHIFLNSALRATAPYKFLSEQHGVKAVF
  MNKHDWYEEYPTTPSSFVLNGFMYSLIGLYDLKETAGEKLGKEARSLYERGMESLKAMLPLYDTGSGTIY
  DLRHFMLGIAPNLARWDYHTTHINQLQLLSTIDESPVFKEFVKRWKSYLKGSRAKHN
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015554
• ORF Size: 1851 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015554.3
• RefSeq Size: 5043 bp
• RefSeq ORF: 1854 bp
• Locus ID: 26035
• UniProt ID: O94923
• Cytogenetics: 15q23
• Protein Families: Transmembrane
• Protein Pathways: Heparan sulfate biosynthesis, Metabolic pathways
• Gene Summary: Converts D-glucuronic acid residues adjacent to N-sulfate sugar residues to L-iduronic acid residues, both in maturing heparan sulfate (HS) and heparin chains. This is important for further modifications that determine the specificity of interactions between these glycosaminoglycans and proteins.[UniProtKB/Swiss-Prot Function]