CCDC64B (BICDL2) (NM_001103175) Human Tagged ORF Clone

CAT#: RG211748

CCDC64B (tGFP-tagged) - Human coiled-coil domain containing 64B (CCDC64B)

Product Images

Circular map for RG211748

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CCDC64B
• Synonyms: BICDR-2; BICDR2; CCDC64B
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG211748 representing NM_001103175
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTCTCCAGATGGGCCCAGCTTCCCGTCCGGGCCGCTCTCAGGGGGCGCCTCTCCCAGCGGCGACG
  AGGGCTTCTTCCCCTTTGTGCTGGAGCGGCGGGACTCATTCCTGGGAGGGGGCCCAGGGCCTGAGGAGCC
  CGAGGACCTAGCCTTGCAGCTGCAGCAGAAGGAGAAAGACCTGCTGTTGGCCGCGGAGCTCGGCAAGATG
  CTTCTGGAGCGAAATGAAGAGCTGCGGCGGCAGCTGGAGACGCTGAGCGCCCAGCACTTGGAGCGTGAGG
  AACGGCTCCAGCAGGAGAACCATGAGCTCCGGCGAGGCCTGGCAGCCCGAGGAGCCGAGTGGGAGGCCCG
  GGCCGTGGAGCTGGAGGGGGACGTGGAGGCCCTGCGGGCCCAGCTTGGGGAGCAGCGCTCAGAGCAGCAG
  GACAGTGGGCGAGAACGGGCACGGGCCCTCAGCGAGCTCAGCGAGCAGAACCTCCGGCTCAGCCAGCAGC
  TGGCTCAGGCCTCCCAGACTGAGCAGGAACTTCAGAGGGAACTGGACGCCCTTCGGGGACAGTGCCAGGC
  TCAGGCACTGGCTGGAGCAGAGCTGAGGACGCGGCTGGAGAGTCTGCAGGGGGAAAACCAGATGCTGCAG
  AGCCGCCGGCAGGACCTGGAGGCCCAGATCCGAGGCCTGCGTGAGGAGGTGGAGAAGGGTGAGGGCAGAC
  TGCAGACCACCCACGAGGAGTTGCTGCTGCTGAGGCGGGAGCGGCGGGAGCACAGCCTGGAGCTGGAACG
  CGCACGGTCAGAGGCTGGGGAGGCGCTGAGTGCGCTGCGGAGGCTGCAGCGGCGCGTCTCCGAGCTGGAG
  GAGGAGTCACGCCTCCAGGACGCCGACGTGTCGGCTGCCTCGTTGCAGTCAGAACTGGCCCACAGCCTCG
  ACGACGGCGACCAGGGCCAGGGCGCCGACGCACCCGGAGACACCCCGACCACCCGGTCCCCAAAGACCCG
  AAAGGCGTCCAGCCCCCAGCCTTCACCCCCGGAAGAGATCTTAGAGCCCCCCAAGAAGCGAACATCCCTC
  AGTCCAGCGGAGATACTGGAGGAGAAGGAGGTGGAAGTGGCCAAGCTGCAAGATGAGATCTCGCTGCAGC
  AGGCAGAGCTGCAGTCCCTGCGGGAAGAGCTGCAGAGGCAGAAGGAGCTGCGGGCGCAGGAAGACCCTGG
  GGAGGCCCTGCACAGTGCCCTCTCAGACCGGGACGAGGCCGTGAACAAGGCCCTGGAGCTGTCCCTGCAG
  CTCAACCGCGTCTCGCTGGAGCGAGACTCCCTGTCTCGGGAGCTGCTGCGCGCCATCCGCCAGAAGGTGG
  CGCTCACGCAGGAGCTGGAGGCCTGGCAGGACGACATGCAGGTGGTGATCGGGCAGCAGCTGCGCTCACA
  GCGCCAGAAGGAGCTGAGCGCCTCCGCGTCGTCGTCGACCCCGCGCCGTGCCGCGCCCCGCTTCTCGCTG
  CGCCTGGGCCCCGGGCCCGCCGGTGGCTTTCTCAGTAACCTCTTCCGAAGGACC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG211748 representing NM_001103175
  Red=Cloning site Green=Tags(s)
  
  MSSPDGPSFPSGPLSGGASPSGDEGFFPFVLERRDSFLGGGPGPEEPEDLALQLQQKEKDLLLAAELGKM
  LLERNEELRRQLETLSAQHLEREERLQQENHELRRGLAARGAEWEARAVELEGDVEALRAQLGEQRSEQQ
  DSGRERARALSELSEQNLRLSQQLAQASQTEQELQRELDALRGQCQAQALAGAELRTRLESLQGENQMLQ
  SRRQDLEAQIRGLREEVEKGEGRLQTTHEELLLLRRERREHSLELERARSEAGEALSALRRLQRRVSELE
  EESRLQDADVSAASLQSELAHSLDDGDQGQGADAPGDTPTTRSPKTRKASSPQPSPPEEILEPPKKRTSL
  SPAEILEEKEVEVAKLQDEISLQQAELQSLREELQRQKELRAQEDPGEALHSALSDRDEAVNKALELSLQ
  LNRVSLERDSLSRELLRAIRQKVALTQELEAWQDDMQVVIGQQLRSQRQKELSASASSSTPRRAAPRFSL
  RLGPGPAGGFLSNLFRRT
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001103175
• ORF Size: 1524 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001103175.2
• RefSeq Size: 1811 bp
• RefSeq ORF: 1527 bp
• Locus ID: 146439
• UniProt ID: A1A5D9
• Cytogenetics: 16p13.3