RIC8 (RIC8A) (NM_021932) Human Tagged ORF Clone

CAT#: RG211359

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  • TrueORF®

RIC8A (tGFP-tagged) - Human resistance to inhibitors of cholinesterase 8 homolog A (C. elegans) (RIC8A)



Reconstitution Protocol

USD 700.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Symbol RIC8A
Synonyms RIC8
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG211359 representing NM_021932
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCCCCGGGCGGTTGCAGAAGCCGTGGAGACGGGTGAGGAGGATGTGATTATGGAAGCTCTGCGGT
CATACAACCAGGAGCACTCCCAGAGCTTCACGTTTGATGATGCCCAACAGGAGGACCGGAAGAGACTGGC
GGAGCTGCTGGTCTCCGTCCTGGAACAGGGCTTGCCACCCTCCCACCGTGTCATCTGGCTGCAGAGTGTC
CGAATCCTGTCCCGGGACCGCAACTGCCTGGACCCGTTCACCAGCCGCCAGAGCCTGCAGGCACTAGCCT
GCTATGCTGACATCTCTGTCTCTGAGGGGTCCGTCCCAGAGTCCGCAGACATGGATGTTGTACTGGAGTC
CCTCAAGTGCCTGTGCAACCTCGTGCTCAGCAGCCCTGTGGCACAGATGCTGGCAGCAGAGGCCCGCCTA
GTGGTGAAGCTCACAGAGCGTGTGGGGCTGTACCGTGAGAGGAGCTTCCCCCACGATGTCCAGTTCTTTG
ACTTGCGGCTCCTCTTCCTGCTAACGGCACTCCGCACCGATGTGCGCCAGCAGCTGTTTCAGGAGCTGAA
AGGAGTGCGCCTGCTAACTGACACACTGGAGCTGACGCTGGGGGTGACTCCTGAAGGGAACCCCCCACCC
ACGCTCCTTCCTTCCCAAGAGACTGAGCGGGCCATGGAGATCCTCAAAGTGCTCTTCAACATCACCCTGG
ACTCCATCAAGGGGGAGGTGGACGAGGAAGACGCTGCCCTTTACCGACACCTGGGGACCCTTCTCCGGCA
CTGTGTGATGATCGCTACTGCTGGAGACCGCACAGAGGAGTTCCACGGCCACGCAGTGAACCTCCTGGGG
AACTTGCCCCTCAAGTGTCTGGATGTTCTCCTCACCCTGGAGCCACATGGAGACTCCACGGAGTTCATGG
GAGTGAATATGGATGTGATTCGTGCCCTCCTCATCTTCCTAGAGAAGCGTTTGCACAAGACACACAGGCT
GAAGGAGAGTGTAGCTCCCGTGCTGAGCGTGCTGACTGAATGTGCCCGGATGCACCGCCCAGCCAGGAAG
TTCCTGAAGGCCCAGGGATGGCCACCTCCCCAGGTGCTGCCCCCTCTGCGGGATGTGAGGACACGGCCTG
AGGTTGGGGAGATGCTGCGGAACAAGCTTGTCCGCCTCATGACACACCTGGACACAGATGTGAAGAGGGT
GGCTGCCGAGTTCTTGTTTGTCCTGTGCTCTGAGAGTGTGCCCCGATTCATCAAGTACACAGGCTATGGG
AATGCTGCTGGCCTTCTGGCTGCCAGGGGCCTCATGGCAGGAGGCCGGCCCGAGGGCCAGTACTCAGAGG
ATGAGGACACAGACACAGATGAGTACAAGGAAGCCAAAGCCAGCATAAACCCTGTGACCGGGAGGGTGGA
GGAGAAGCCGCCTAACCCTATGGAGGGCATGACAGAGGAGCAGAAGGAGCACGAGGCCATGAAGCTGGTG
ACCATGTTTGACAAGCTCTCCAGGAACAGAGTCATCCAGCCAATGGGGATGAGTCCCCGGGGTCATCTTA
CGTCCCTGCAGGATGCCATGTGCGAGACTATGGAGCAGCAGCTCTCCTCGGACCCTGACTCGGACCCTGA
C


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG211359 representing NM_021932
Red=Cloning site Green=Tags(s)

MEPRAVAEAVETGEEDVIMEALRSYNQEHSQSFTFDDAQQEDRKRLAELLVSVLEQGLPPSHRVIWLQSV
RILSRDRNCLDPFTSRQSLQALACYADISVSEGSVPESADMDVVLESLKCLCNLVLSSPVAQMLAAEARL
VVKLTERVGLYRERSFPHDVQFFDLRLLFLLTALRTDVRQQLFQELKGVRLLTDTLELTLGVTPEGNPPP
TLLPSQETERAMEILKVLFNITLDSIKGEVDEEDAALYRHLGTLLRHCVMIATAGDRTEEFHGHAVNLLG
NLPLKCLDVLLTLEPHGDSTEFMGVNMDVIRALLIFLEKRLHKTHRLKESVAPVLSVLTECARMHRPARK
FLKAQGWPPPQVLPPLRDVRTRPEVGEMLRNKLVRLMTHLDTDVKRVAAEFLFVLCSESVPRFIKYTGYG
NAAGLLAARGLMAGGRPEGQYSEDEDTDTDEYKEAKASINPVTGRVEEKPPNPMEGMTEEQKEHEAMKLV
TMFDKLSRNRVIQPMGMSPRGHLTSLQDAMCETMEQQLSSDPDSDPD

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_021932
ORF Size 1611 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_021932.5
RefSeq Size 2714 bp
RefSeq ORF 1614 bp
Locus ID 60626
UniProt ID Q9NPQ8
Cytogenetics 11p15.5
Gene Summary Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins. Able to activate GNAI1, GNAO1 and GNAQ, but not GNAS by exchanging bound GDP for free GTP. Involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein, possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex (By similarity). Also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation.[UniProtKB/Swiss-Prot Function]
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