RIC8 (RIC8A) (NM_021932) Human Tagged ORF Clone

CAT#: RG211359

RIC8A (tGFP-tagged) - Human resistance to inhibitors of cholinesterase 8 homolog A (C. elegans) (RIC8A)

Product Images

Circular map for RG211359

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: RIC8
• Synonyms: RIC8
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG211359 representing NM_021932
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCCCGGGCGGTTGCAGAAGCCGTGGAGACGGGTGAGGAGGATGTGATTATGGAAGCTCTGCGGT
  CATACAACCAGGAGCACTCCCAGAGCTTCACGTTTGATGATGCCCAACAGGAGGACCGGAAGAGACTGGC
  GGAGCTGCTGGTCTCCGTCCTGGAACAGGGCTTGCCACCCTCCCACCGTGTCATCTGGCTGCAGAGTGTC
  CGAATCCTGTCCCGGGACCGCAACTGCCTGGACCCGTTCACCAGCCGCCAGAGCCTGCAGGCACTAGCCT
  GCTATGCTGACATCTCTGTCTCTGAGGGGTCCGTCCCAGAGTCCGCAGACATGGATGTTGTACTGGAGTC
  CCTCAAGTGCCTGTGCAACCTCGTGCTCAGCAGCCCTGTGGCACAGATGCTGGCAGCAGAGGCCCGCCTA
  GTGGTGAAGCTCACAGAGCGTGTGGGGCTGTACCGTGAGAGGAGCTTCCCCCACGATGTCCAGTTCTTTG
  ACTTGCGGCTCCTCTTCCTGCTAACGGCACTCCGCACCGATGTGCGCCAGCAGCTGTTTCAGGAGCTGAA
  AGGAGTGCGCCTGCTAACTGACACACTGGAGCTGACGCTGGGGGTGACTCCTGAAGGGAACCCCCCACCC
  ACGCTCCTTCCTTCCCAAGAGACTGAGCGGGCCATGGAGATCCTCAAAGTGCTCTTCAACATCACCCTGG
  ACTCCATCAAGGGGGAGGTGGACGAGGAAGACGCTGCCCTTTACCGACACCTGGGGACCCTTCTCCGGCA
  CTGTGTGATGATCGCTACTGCTGGAGACCGCACAGAGGAGTTCCACGGCCACGCAGTGAACCTCCTGGGG
  AACTTGCCCCTCAAGTGTCTGGATGTTCTCCTCACCCTGGAGCCACATGGAGACTCCACGGAGTTCATGG
  GAGTGAATATGGATGTGATTCGTGCCCTCCTCATCTTCCTAGAGAAGCGTTTGCACAAGACACACAGGCT
  GAAGGAGAGTGTAGCTCCCGTGCTGAGCGTGCTGACTGAATGTGCCCGGATGCACCGCCCAGCCAGGAAG
  TTCCTGAAGGCCCAGGGATGGCCACCTCCCCAGGTGCTGCCCCCTCTGCGGGATGTGAGGACACGGCCTG
  AGGTTGGGGAGATGCTGCGGAACAAGCTTGTCCGCCTCATGACACACCTGGACACAGATGTGAAGAGGGT
  GGCTGCCGAGTTCTTGTTTGTCCTGTGCTCTGAGAGTGTGCCCCGATTCATCAAGTACACAGGCTATGGG
  AATGCTGCTGGCCTTCTGGCTGCCAGGGGCCTCATGGCAGGAGGCCGGCCCGAGGGCCAGTACTCAGAGG
  ATGAGGACACAGACACAGATGAGTACAAGGAAGCCAAAGCCAGCATAAACCCTGTGACCGGGAGGGTGGA
  GGAGAAGCCGCCTAACCCTATGGAGGGCATGACAGAGGAGCAGAAGGAGCACGAGGCCATGAAGCTGGTG
  ACCATGTTTGACAAGCTCTCCAGGAACAGAGTCATCCAGCCAATGGGGATGAGTCCCCGGGGTCATCTTA
  CGTCCCTGCAGGATGCCATGTGCGAGACTATGGAGCAGCAGCTCTCCTCGGACCCTGACTCGGACCCTGA
  C
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG211359 representing NM_021932
  Red=Cloning site Green=Tags(s)
  
  MEPRAVAEAVETGEEDVIMEALRSYNQEHSQSFTFDDAQQEDRKRLAELLVSVLEQGLPPSHRVIWLQSV
  RILSRDRNCLDPFTSRQSLQALACYADISVSEGSVPESADMDVVLESLKCLCNLVLSSPVAQMLAAEARL
  VVKLTERVGLYRERSFPHDVQFFDLRLLFLLTALRTDVRQQLFQELKGVRLLTDTLELTLGVTPEGNPPP
  TLLPSQETERAMEILKVLFNITLDSIKGEVDEEDAALYRHLGTLLRHCVMIATAGDRTEEFHGHAVNLLG
  NLPLKCLDVLLTLEPHGDSTEFMGVNMDVIRALLIFLEKRLHKTHRLKESVAPVLSVLTECARMHRPARK
  FLKAQGWPPPQVLPPLRDVRTRPEVGEMLRNKLVRLMTHLDTDVKRVAAEFLFVLCSESVPRFIKYTGYG
  NAAGLLAARGLMAGGRPEGQYSEDEDTDTDEYKEAKASINPVTGRVEEKPPNPMEGMTEEQKEHEAMKLV
  TMFDKLSRNRVIQPMGMSPRGHLTSLQDAMCETMEQQLSSDPDSDPD
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021932
• ORF Size: 1611 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021932.5
• RefSeq Size: 2714 bp
• RefSeq ORF: 1614 bp
• Locus ID: 60626
• UniProt ID: Q9NPQ8
• Cytogenetics: 11p15.5
• Gene Summary: Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins. Able to activate GNAI1, GNAO1 and GNAQ, but not GNAS by exchanging bound GDP for free GTP. Involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein, possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex (By similarity). Also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation.[UniProtKB/Swiss-Prot Function]