Pumilio 2 (PUM2) (NM_015317) Human Tagged ORF Clone

CAT#: RG211307

PUM2 (tGFP-tagged) - Human pumilio homolog 2 (Drosophila) (PUM2)

Product Images

Circular map for RG211307

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Pumilio 2
• Synonyms: PUMH2; PUML2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG211307 representing NM_015317
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATCATGATTTTCAAGCTCTTGCATTAGAATCTCGGGGAATGGGAGAGCTTTTGCCTACCAAAAAGT
  TTTGGGAACCTGATGATTCAACAAAAGATGGACAAAAAGGCATATTTCTTGGGGATGATGAATGGAGAGA
  GACTGCATGGGGAGCTTCTCACCATTCAATGTCCCAGCCTATTATGGTACAGAGAAGATCTGGACAGGGT
  TTTCATGGAAACAGTGAAGTAAATGCAATACTGTCTCCGCGATCAGAAAGTGGAGGCCTTGGTGTGAGCA
  TGGTAGAATATGTATTAAGTTCTTCTCCTGCTGATAAATTGGATTCTCGATTTAGGAAGGGAAATTTTGG
  CACTAGAGATGCTGAAACAGATGGACCTGAGAAAGGAGATCAAAAAGGCAAGGCTTCTCCATTTGAGGAG
  GACCAAAACAGAGATCTTAAACAAGGAGATGATGATGATTCTAAAATAAATGGCAGAGGTTTGCCAAATG
  GAATGGATGCCGATTGCAAAGATTTTAATCGTACTCCTGGAAGTCGTCAAGCCTCTCCAACTGAAGTAGT
  TGAGCGCTTGGGCCCCAATACTAATCCCTCAGAAGGACTGGGGCCTCTTCCTAATCCTACAGCTAATAAA
  CCACTTGTTGAAGAATTTTCAAATCCTGAAACTCAGAATCTGGATGCCATGGAACAAGTTGGTCTGGAAT
  CCTTACAGTTTGACTATCCTGGTAATCAGGTACCAATGGACTCTTCAGGAGCTACTGTAGGCCTTTTTGA
  CTACAATTCCCAGCAGCAGCTCTTTCAGAGGACTAATGCACTAACAGTTCAACAGTTAACTGCAGCTCAA
  CAGCAGCAATATGCATTAGCAGCAGCTCAGCAGCCACATATAGCTGGTGTATTCTCAGCAGGCCTTGCTC
  CAGCTGCATTTGTGCCAAATCCATACATTATTAGTGCTGCTCCTCCAGGGACCGATCCGTATACTGCAGC
  AGGATTGGCTGCAGCAGCTACATTAGCAGGTCCAGCAGTGGTTCCACCTCAGTATTACGGCGTTCCATGG
  GGGGTGTATCCAGCCAACTTATTTCAGCAGCAAGCTGCAGCTGCGGCAAATAACACAGCCAGTCAGCAAG
  CAGCATCACAAGCTCAGCCTGGACAGCAACAGGTTCTCCGTGCTGGAGCAGGTCAGCGTCCTCTTACTCC
  CAATCAGGGTCAGCAAGGGCAGCAAGCAGAATCACTTGCGGCAGCTGCAGCAGCAAATCCAACATTGGCT
  TTTGGTCAGGGTCTTGCTACTGGCATGCCAGGCTATCAAGTACTAGCTCCAACTGCCTATTATGATCAGA
  CTGGTGCCTTAGTGGTTGGCCCTGGAGCAAGGACTGGCCTTGGAGCTCCAGTTCGGTTAATGGCTCCAAC
  ACCTGTTTTAATTAGTTCAGCAGCAGCACAAGCTGCAGCAGCAGCAGCAGCTGGAGGAACTGCAAGTAGC
  CTTACAGGCAGCACAAATGGTCTGTTTCGGCCAATTGGCACTCAGCCACCACAGCAGCAGCAACAGCAGC
  CAAGCACTAATCTGCAATCTAATTCATTTTATGGAAGCAGTTCTTTGACTAATAGCTCCCAGAGTAGTTC
  TTTATTTTCTCATGGACCTGGTCAACCTGGAAGTACATCTCTTGGCTTTGGAAGTGGTAACTCTTTGGGT
  GCTGCTATAGGCTCAGCCCTCAGTGGATTTGGTTCATCAGTTGGCAGTTCTGCAAGTAGTAGTGCCACAA
  GGAGAGAGTCTCTATCTACTAGCTCTGACTTGTACAAAAGATCTAGTAGCAGCCTAGCACCCATAGGGCA
  ACCATTTTACAATAGTCTGGGATTTTCCTCCTCTCCAAGTCCAATAGGCATGCCTCTGCCAAGCCAAACT
  CCAGGACATTCACTTACGCCACCGCCATCACTTTCATCACATGGATCCTCATCCAGTTTGCATTTAGGAG
  GACTGACAAATGGTAGTGGTCGATATATCTCTGCAGCACCTGGAGCAGAAGCAAAATATCGAAGTGCTTC
  AAGCACTTCCAGTCTATTTAGCTCCAGCAGCCAGCTCTTTCCTCCTTCCCGGCTTCGGTATAATAGGTCT
  GATATTATGCCTTCTGGCCGCAGTAGATTATTGGAAGATTTCAGAAACAACCGCTTCCCAAACCTTCAGC
  TTAGAGACTTGATTGGACATATAGTTGAGTTTTCTCAAGACCAGCATGGTTCTAGATTCATACAGCAAAA
  ACTAGAGAGAGCTACTCCAGCTGAGCGACAGATGGTATTTAATGAAATTCTGCAAGCAGCCTATCAATTA
  ATGACTGATGTTTTTGGCAACTATGTTATACAGAAGTTTTTTGAGTTTGGGAGTCTGGATCAAAAATTAG
  CCCTGGCTACTCGTATTCGTGGTCATGTTCTACCCTTAGCCTTGCAGATGTATGGCTGCCGCGTTATTCA
  GAAAGCATTAGAATCTATTTCTTCTGACCAGCAGAGTGAAATGGTAAAGGAGCTGGATGGTCATGTGCTC
  AAATGTGTGAAAGATCAGAATGGAAACCATGTTGTACAAAAATGTATCGAATGTGTTCAGCCACAGTCAC
  TACAGTTCATCATTGATGCTTTCAAGGGACAAGTATTTGTGCTTTCAACTCATCCTTATGGCTGCAGAGT
  AATTCAGCGCATCCTAGAGCATTGCACTGCAGAACAGACCTTACCTATCTTAGAAGAACTCCACCAACAT
  ACAGAGCAGTTGGTACAGGATCAGTATGGCAATTATGTTATTCAGCATGTACTGGAACACGGTCGACCTG
  AAGACAAGAGCAAAATTGTTTCCGAAATCAGGGGAAAGGTTTTAGCCCTGAGTCAACACAAATTTGCCAG
  CAATGTAGTAGAAAAGTGTGTTACTCATGCCTCCCGTGCTGAGAGAGCTTTACTGATTGACGAGGTTTGC
  TGCCAGAATGATGGTCCTCACAGTGCCTTATACACCATGATGAAGGACCAGTATGCCAATTACGTGGTTC
  AAAAGATGATTGATATGGCTGAACCTGCTCAGAGAAAGATAATCATGCACAAGATTCGACCTCACATTAC
  TACTTTGCGCAAATACACATACGGGAAGCATATACTGGCCAAGTTGGAAAAGTATTATTTGAAGAATAGC
  CCGGACCTAGGACCTATTGGAGGACCACCAAATGGAATGCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG211307 representing NM_015317
  Red=Cloning site Green=Tags(s)
  
  MNHDFQALALESRGMGELLPTKKFWEPDDSTKDGQKGIFLGDDEWRETAWGASHHSMSQPIMVQRRSGQG
  FHGNSEVNAILSPRSESGGLGVSMVEYVLSSSPADKLDSRFRKGNFGTRDAETDGPEKGDQKGKASPFEE
  DQNRDLKQGDDDDSKINGRGLPNGMDADCKDFNRTPGSRQASPTEVVERLGPNTNPSEGLGPLPNPTANK
  PLVEEFSNPETQNLDAMEQVGLESLQFDYPGNQVPMDSSGATVGLFDYNSQQQLFQRTNALTVQQLTAAQ
  QQQYALAAAQQPHIAGVFSAGLAPAAFVPNPYIISAAPPGTDPYTAAGLAAAATLAGPAVVPPQYYGVPW
  GVYPANLFQQQAAAAANNTASQQAASQAQPGQQQVLRAGAGQRPLTPNQGQQGQQAESLAAAAAANPTLA
  FGQGLATGMPGYQVLAPTAYYDQTGALVVGPGARTGLGAPVRLMAPTPVLISSAAAQAAAAAAAGGTASS
  LTGSTNGLFRPIGTQPPQQQQQQPSTNLQSNSFYGSSSLTNSSQSSSLFSHGPGQPGSTSLGFGSGNSLG
  AAIGSALSGFGSSVGSSASSSATRRESLSTSSDLYKRSSSSLAPIGQPFYNSLGFSSSPSPIGMPLPSQT
  PGHSLTPPPSLSSHGSSSSLHLGGLTNGSGRYISAAPGAEAKYRSASSTSSLFSSSSQLFPPSRLRYNRS
  DIMPSGRSRLLEDFRNNRFPNLQLRDLIGHIVEFSQDQHGSRFIQQKLERATPAERQMVFNEILQAAYQL
  MTDVFGNYVIQKFFEFGSLDQKLALATRIRGHVLPLALQMYGCRVIQKALESISSDQQSEMVKELDGHVL
  KCVKDQNGNHVVQKCIECVQPQSLQFIIDAFKGQVFVLSTHPYGCRVIQRILEHCTAEQTLPILEELHQH
  TEQLVQDQYGNYVIQHVLEHGRPEDKSKIVSEIRGKVLALSQHKFASNVVEKCVTHASRAERALLIDEVC
  CQNDGPHSALYTMMKDQYANYVVQKMIDMAEPAQRKIIMHKIRPHITTLRKYTYGKHILAKLEKYYLKNS
  PDLGPIGGPPNGML
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015317
• ORF Size: 3192 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015317.5
• RefSeq Size: 6111 bp
• RefSeq ORF: 3195 bp
• Locus ID: 23369
• UniProt ID: Q8TB72
• Cytogenetics: 2p24.1
• Domains: PUF
• Gene Summary: This gene encodes a protein that belongs to a family of RNA-binding proteins. The encoded protein functions as a translational repressor during embryonic development and cell differentiation. This protein is also thought to be a positive regulator of cell proliferation in adipose-derived stem cells. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]