HNRPA3 (HNRNPA3) (NM_194247) Human Tagged ORF Clone

CAT#: RG211304

HNRNPA3 (tGFP-tagged) - Human heterogeneous nuclear ribonucleoprotein A3 (HNRNPA3). Note: ORF is codon optimized

Product Images

Circular map for RG211304

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: HNRPA3
• Synonyms: 2610510D13Rik; D10S102; FBRNP; HNRPA3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG211304 ORF sequence, codon optimized.
  Due to the complexity of NM_194247, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGTAAAACCGCCGCCCGGTCGCCCCCAGCCCGACTCCGGCCGTCGCCGTCGCCGCCGGGGGGAGG
  AGGGCCATGATCCAAAGGAACCAGAGCAGTTGAGAAAACTGTTTATTGGTGGTCTGAGCTTTGAAACTAC
  AGATGATAGTTTACGAGAACATTTTGAGAAATGGGGCACACTCACAGATTGTGTGGTAATGAGAGACCCC
  CAAACAAAACGTTCCAGGGGCTTTGGTTTTGTGACTTATTCTTGTGTTGAAGAGGTGGATGCAGCAATGT
  GTGCTCGACCACACAAGGTTGATGGGCGTGTAGTGGAACCAAAGAGAGCTGTTTCTAGAGAGGATTCTGT
  AAAGCCTGGTGCCCATCTAACAGTGAAGAAAATTTTTGTTGGTGGTATTAAAGAAGATACAGAAGAATAT
  AATTTGAGAGACTACTTTGAAAAGTATGGCAAGATTGAAACCATAGAAGTTATGGAAGACAGGCAGAGTG
  GAAAAAAGAGAGGATTTGCTTTTGTAACTTTTGATGATCATGATACAGTTGATAAAATTGTTGTTCAGAA
  ATACCACACTATTAATGGGCATAATTGTGAAGTGAAAAAGGCCCTTTCTAAACAAGAGATGCAGTCTGCT
  GGATCACAGAGAGGTCGTGGAGGTGGATCTGGCAATTTTATGGGTCGCGGAGGGAACTTTGGAGGTGGTG
  GAGGTAATTTTGGCCGTGGTGGAAACTTTGGTGGAAGAGGAGGCTATGGTGGTGGAGGTGGTGGCAGCAG
  AGGTAGTTATGGAGGAGGTGATGGTGGATATAATGGATTTGGAGGTGATGGTGGCAACTATGGCGGTGGT
  CCTGGTTATAGTAGTAGAGGGGGCTATGGTGGTGGTGGACCAGGATATGGAAACCAAGGTGGTGGATATG
  GTGGAGGTGGAGGATATGATGGTTACAATGAAGGAGGAAATTTTGGCGGTGGTAACTATGGTGGTGGTGG
  GAACTATAATGATTTTGGAAATTATAGTGGACAACAGCAATCAAATTATGGACCCATGAAAGGGGGCAGT
  TTTGGTGGAAGAAGCTCGGGCAGTCCCTATGGTGGTGGTTATGGATCTGGTGGTGGAAGTGGTGGATATG
  GTAGCAGAAGGTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG211304 representing NM_194247
  Red=Cloning site Green=Tags(s)
  
  MEVKPPPGRPQPDSGRRRRRRGEEGHDPKEPEQLRKLFIGGLSFETTDDSLREHFEKWGTLTDCVVMRDP
  QTKRSRGFGFVTYSCVEEVDAAMCARPHKVDGRVVEPKRAVSREDSVKPGAHLTVKKIFVGGIKEDTEEY
  NLRDYFEKYGKIETIEVMEDRQSGKKRGFAFVTFDDHDTVDKIVVQKYHTINGHNCEVKKALSKQEMQSA
  GSQRGRGGGSGNFMGRGGNFGGGGGNFGRGGNFGGRGGYGGGGGGSRGSYGGGDGGYNGFGGDGGNYGGG
  PGYSSRGGYGGGGPGYGNQGGGYGGGGGYDGYNEGGNFGGGNYGGGGNYNDFGNYSGQQQSNYGPMKGGS
  FGGRSSGSPYGGGYGSGGGSGGYGSRRF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_194247
• ORF Size: 1134 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_194247.3
• RefSeq Size: 5810 bp
• RefSeq ORF: 1137 bp
• Locus ID: 220988
• UniProt ID: P51991
• Cytogenetics: 2q31.2
• Protein Pathways: Spliceosome
• Gene Summary: Plays a role in cytoplasmic trafficking of RNA. Binds to the cis-acting response element, A2RE. May be involved in pre-mRNA splicing.[UniProtKB/Swiss-Prot Function]