LI Cadherin (CDH17) (NM_004063) Human Tagged ORF Clone

CAT#: RG211298

CDH17 (tGFP-tagged) - Human cadherin 17, LI cadherin (liver-intestine) (CDH17), transcript variant 1

Product Images

Circular map for RG211298

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: LI Cadherin
• Synonyms: CDH16; HPT-1; HPT1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG211298 representing NM_004063
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATACTTCAGGCCCATCTTCACTCCCTGTGTCTTCTTATGCTTTATTTGGCAACTGGATATGGCCAAG
  AGGGGAAGTTTAGTGGACCCCTGAAACCCATGACATTTTCTATTTATGAAGGCCAAGAACCGAGTCAAAT
  TATATTCCAGTTTAAGGCCAATCCTCCTGCTGTGACTTTTGAACTAACTGGGGAGACAGACAACATATTT
  GTGATAGAACGGGAGGGACTTCTGTATTACAACAGAGCCTTGGACAGGGAAACAAGATCTACTCACAATC
  TCCAGGTTGCAGCCCTGGACGCTAATGGAATTATAGTGGAGGGTCCAGTCCCTATCACCATAGAAGTGAA
  GGACATCAACGACAATCGACCCACGTTTCTCCAGTCAAAGTACGAAGGCTCAGTAAGGCAGAACTCTCGC
  CCAGGAAAGCCCTTCTTGTATGTCAATGCCACAGACCTGGATGATCCGGCCACTCCCAATGGCCAGCTTT
  ATTACCAGATTGTCATCCAGCTTCCCATGATCAACAATGTTATGTACTTTCAGATCAACAACAAAACGGG
  AGCCATCTCTCTTACCCGAGAGGGATCTCAGGAATTGAATCCTGCTAAGAATCCTTCCTATAATCTGGTG
  ATCTCAGTGAAGGACATGGGAGGCCAGAGTGAGAATTCCTTCAGTGATACCACATCTGTGGATATCATAG
  TGACAGAGAATATTTGGAAAGCACCAAAACCTGTGGAGATGGTGGAAAACTCAACTGATCCTCACCCCAT
  CAAAATCACTCAGGTGCGGTGGAATGATCCCGGTGCACAATATTCCTTAGTTGACAAAGAGAAGCTGCCA
  AGATTCCCATTTTCAATTGACCAGGAAGGAGATATTTACGTGACTCAGCCCTTGGACCGAGAAGAAAAGG
  ATGCATATGTTTTTTATGCAGTTGCAAAGGATGAGTACGGAAAACCACTTTCATATCCGCTGGAAATTCA
  TGTAAAAGTTAAAGATATTAATGATAATCCACCTACATGTCCGTCACCAGTAACCGTATTTGAGGTCCAG
  GAGAATGAACGACTGGGTAACAGTATCGGGACCCTTACTGCACATGACAGGGATGAAGAAAATACTGCCA
  ACAGTTTTCTAAACTACAGGATTGTGGAGCAAACTCCCAAACTTCCCATGGATGGACTCTTCCTAATCCA
  AACCTATGCTGGAATGTTACAGTTAGCTAAACAGTCCTTGAAGAAGCAAGATACTCCTCAGTACAACTTA
  ACGATAGAGGTGTCTGACAAAGATTTCAAGACCCTTTGTTTTGTGCAAATCAACGTTATTGATATCAATG
  ATCAGATCCCCATCTTTGAAAAATCAGATTATGGAAACCTGACTCTTGCTGAAGACACAAACATTGGGTC
  CACCATCTTAACCATCCAGGCCACTGATGCTGATGAGCCATTTACTGGGAGTTCTAAAATTCTGTATCAT
  ATCATAAAGGGAGACAGTGAGGGACGCCTGGGGGTTGACACAGATCCCCATACCAACACCGGATATGTCA
  TAATTAAAAAGCCTCTTGATTTTGAAACAGCAGCTGTTTCCAACATTGTGTTCAAAGCAGAAAATCCTGA
  GCCTCTAGTGTTTGGTGTGAAGTACAATGCAAGTTCTTTTGCCAAGTTCACGCTTATTGTGACAGATGTG
  AATGAAGCACCTCAATTTTCCCAACACGTATTCCAAGCGAAAGTCAGTGAGGATGTAGCTATAGGCACTA
  AAGTGGGCAATGTGACTGCCAAGGATCCAGAAGGTCTGGACATAAGTTATTCACTGAGGGGAGACACAAG
  AGGTTGGCTTAAAATTGACCACGTGACTGGTGAGATCTTTAGTGTGGCTCCATTGGACAGAGAAGCCGGA
  AGTCCATATCGGGTACAAGTGGTGGCCACAGAAGTAGGGGGGTCTTCCTTGAGCTCTGTGTCAGAGTTCC
  ACCTGATCCTTATGGATGTGAATGACAACCCTCCCAGGCTAGCCAAGGACTACACGGGCTTGTTCTTCTG
  CCATCCCCTCAGTGCACCTGGAAGTCTCATTTTTGAGGCTACTGATGATGATCAGCACTTATTTCGGGGT
  CCCCATTTTACATTTTCCCTCGGCAGTGGAAGCTTACAAAACGACTGGGAAGTTTCCAAAATCAATGGTA
  CTCATGCCCGACTGTCTACCAGGCACACAGACTTTGAGGAGAGGGAGTATGTCGTCTTGATCCGCATCAA
  TGATGGGGGTCGGCCACCCTTGGAAGGCATTGTTTCTTTACCAGTTACATTCTGCAGTTGTGTGGAAGGA
  AGTTGTTTCCGGCCAGCAGGTCACCAGACTGGGATACCCACTGTGGGCATGGCAGTTGGTATACTGCTGA
  CCACCCTTCTGGTGATTGGTATAATTTTAGCAGTTGTGTTTATCCGCATAAAGAAGGATAAAGGCAAAGA
  TAATGTTGAAAGTGCTCAAGCATCTGAAGTCAAACCTCTGAGAAGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG211298 representing NM_004063
  Red=Cloning site Green=Tags(s)
  
  MILQAHLHSLCLLMLYLATGYGQEGKFSGPLKPMTFSIYEGQEPSQIIFQFKANPPAVTFELTGETDNIF
  VIEREGLLYYNRALDRETRSTHNLQVAALDANGIIVEGPVPITIEVKDINDNRPTFLQSKYEGSVRQNSR
  PGKPFLYVNATDLDDPATPNGQLYYQIVIQLPMINNVMYFQINNKTGAISLTREGSQELNPAKNPSYNLV
  ISVKDMGGQSENSFSDTTSVDIIVTENIWKAPKPVEMVENSTDPHPIKITQVRWNDPGAQYSLVDKEKLP
  RFPFSIDQEGDIYVTQPLDREEKDAYVFYAVAKDEYGKPLSYPLEIHVKVKDINDNPPTCPSPVTVFEVQ
  ENERLGNSIGTLTAHDRDEENTANSFLNYRIVEQTPKLPMDGLFLIQTYAGMLQLAKQSLKKQDTPQYNL
  TIEVSDKDFKTLCFVQINVIDINDQIPIFEKSDYGNLTLAEDTNIGSTILTIQATDADEPFTGSSKILYH
  IIKGDSEGRLGVDTDPHTNTGYVIIKKPLDFETAAVSNIVFKAENPEPLVFGVKYNASSFAKFTLIVTDV
  NEAPQFSQHVFQAKVSEDVAIGTKVGNVTAKDPEGLDISYSLRGDTRGWLKIDHVTGEIFSVAPLDREAG
  SPYRVQVVATEVGGSSLSSVSEFHLILMDVNDNPPRLAKDYTGLFFCHPLSAPGSLIFEATDDDQHLFRG
  PHFTFSLGSGSLQNDWEVSKINGTHARLSTRHTDFEEREYVVLIRINDGGRPPLEGIVSLPVTFCSCVEG
  SCFRPAGHQTGIPTVGMAVGILLTTLLVIGIILAVVFIRIKKDKGKDNVESAQASEVKPLRS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004063
• ORF Size: 2496 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004063.2, NP_004054.2
• RefSeq Size: 3697 bp
• RefSeq ORF: 2499 bp
• Locus ID: 1015
• UniProt ID: Q12864
• Cytogenetics: 8q22.1
• Protein Families: Transmembrane
• Gene Summary: This gene is a member of the cadherin superfamily, genes encoding calcium-dependent, membrane-associated glycoproteins. The encoded protein is cadherin-like, consisting of an extracellular region, containing 7 cadherin domains, and a transmembrane region but lacking the conserved cytoplasmic domain. The protein is a component of the gastrointestinal tract and pancreatic ducts, acting as an intestinal proton-dependent peptide transporter in the first step in oral absorption of many medically important peptide-based drugs. The protein may also play a role in the morphological organization of liver and intestine. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2009]