NLRP10 (NM_176821) Human Tagged ORF Clone

CAT#: RG211215

NLRP10 (tGFP-tagged) - Human NLR family, pyrin domain containing 10 (NLRP10)

Product Images

Circular map for RG211215

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: NLRP10
• Synonyms: CLR11.1; NALP10; NOD8; PAN5; PYNOD
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG211215 representing NM_176821
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCATGGCCAAGGCCAGAAAGCCCCGGGAGGCATTGCTCTGGGCCTTGAGTGACCTTGAGGAGAACG
  ATTTCAAGAAGTTAAAGTTCTACTTACGGGATATGACCCTGTCTGAGGGCCAGCCCCCACTGGCCAGAGG
  GGAGTTGGAGGGCCTGATTCCGGTGGACCTGGCAGAATTACTGATTTCAAAGTATGGAGAAAAGGAGGCT
  GTGAAAGTTGTCCTCAAGGGCTTGAAGGTCATGAACCTGTTGGAACTTGTGGACCAGCTCAGCCATATTT
  GTCTGCATGATTACAGAGAAGTATACCGAGAGCATGTGCGCTGCCTAGAGGAATGGCAGGAAGCAGGAGT
  CAATGGCAGATACAACCAGGTGCTCCTGGTGGCCAAGCCCAGCTCAGAGAGCCCAGAATCACTTGCCTGC
  CCCTTCCCGGAGCAGGAGCTGGAGTCTGTCACGGTGGAGGCTCTATTTGATTCAGGGGAAAAGCCCTCAC
  TGGCCCCATCCTTAGTTGTGCTACAGGGGTCGGCTGGCACTGGAAAGACAACTCTCGCCAGAAAAATGGT
  GTTGGACTGGGCCACCGGTACTCTGTACCCAGGCCGGTTTGATTATGTCTTTTATGTAAGCTGCAAAGAA
  GTGGTCCTGCTGCTGGAGAGCAAACTGGAGCAGCTCCTTTTCTGGTGCTGCGGGGACAATCAAGCCCCTG
  TCACAGAGATTCTGAGGCAGCCAGAGCGGCTCCTGTTCATCCTGGATGGCTTTGATGAGCTGCAGAGGCC
  CTTTGAAGAAAAGTTGAAGAAGAGGGGTTTGAGTCCCAAGGAGAGCCTGCTGCACCTTCTAATTAGGAGA
  CATACACTCCCCACGTGCTCCCTTCTCATCACCACCCGGCCCCTGGCTTTGAGGAATCTGGAGCCCTTGC
  TGAAACAAGCACGTCATGTCCATATCCTAGGCTTCTCTGAGGAGGAGAGGGCGAGGTACTTCAGCTCCTA
  TTTCACGGATGAGAAGCAAGCTGACCGTGCCTTCGACATTGTACAGAAAAATGACATTCTCTACAAAGCG
  TGTCAGGTTCCAGGCATTTGCTGGGTGGTCTGCTCCTGGCTGCAGGGGCAGATGGAGAGAGGCAAAGTTG
  TCTTAGAGACACCTAGAAACAGCACTGACATCTTCATGGCTTACGTCTCCACCTTTCTGCCGCCCGATGA
  TGATGGGGGCTGCTCCGAGCTTTCCCGGCACAGGGTCCTGAGGAGTCTGTGCTCCCTAGCAGCTGAAGGG
  ATTCAGCACCAGAGGTTCCTATTTGAAGAAGCTGAGCTCAGGAAACATAATTTAGATGGCCCCAGGCTTG
  CCGCTTTCCTGAGTAGTAACGACTACCAATTGGGACTTGCCATCAAGAAGTTCTACAGCTTCCGCCACAT
  CAGCTTCCAGGACTTTTTTCATGCCATGTCTTACCTGGTGAAAGAGGACCAAAGCCGGCTGGGGAAGGAG
  TCCCGCAGAGAAGTGCAAAGGCTGCTGGAGGTAAAGGAGCAGGAAGGGAATGATGAGATGACCCTCACTA
  TGCAGTTTTTACTGGACATCTCGAAAAAAGACAGCTTCTCGAACTTGGAGCTCAAGTTCTGCTTCAGAAT
  TTCTCCCTGTTTAGCGCAGGATCTGAAGCATTTTAAAGAACAGATGGAATCTATGAAGCACAACAGGACC
  TGGGATTTGGAATTCTCCCTGTATGAAGCTAAAATAAAGAATCTGGTAAAAGGTATTCAGATGAACAATG
  TATCATTCAAGATAAAACATTCAAATGAAAAGAAATCACAGAGCCAGAATTTATTTTCTGTCAAAAGCAG
  CTTGAGTCATGGACCTAAGGAGGAGCAAAAATGTCCTTCTGTCCATGGACAGAAGGAGGGCAAAGATAAT
  ATAGCAGGAACACAAAAGGAAGCTTCTACTGGAAAAGGCAGAGGGACAGAGGAAACACCAAAAAATACTT
  ACATA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG211215 representing NM_176821
  Red=Cloning site Green=Tags(s)
  
  MAMAKARKPREALLWALSDLEENDFKKLKFYLRDMTLSEGQPPLARGELEGLIPVDLAELLISKYGEKEA
  VKVVLKGLKVMNLLELVDQLSHICLHDYREVYREHVRCLEEWQEAGVNGRYNQVLLVAKPSSESPESLAC
  PFPEQELESVTVEALFDSGEKPSLAPSLVVLQGSAGTGKTTLARKMVLDWATGTLYPGRFDYVFYVSCKE
  VVLLLESKLEQLLFWCCGDNQAPVTEILRQPERLLFILDGFDELQRPFEEKLKKRGLSPKESLLHLLIRR
  HTLPTCSLLITTRPLALRNLEPLLKQARHVHILGFSEEERARYFSSYFTDEKQADRAFDIVQKNDILYKA
  CQVPGICWVVCSWLQGQMERGKVVLETPRNSTDIFMAYVSTFLPPDDDGGCSELSRHRVLRSLCSLAAEG
  IQHQRFLFEEAELRKHNLDGPRLAAFLSSNDYQLGLAIKKFYSFRHISFQDFFHAMSYLVKEDQSRLGKE
  SRREVQRLLEVKEQEGNDEMTLTMQFLLDISKKDSFSNLELKFCFRISPCLAQDLKHFKEQMESMKHNRT
  WDLEFSLYEAKIKNLVKGIQMNNVSFKIKHSNEKKSQSQNLFSVKSSLSHGPKEEQKCPSVHGQKEGKDN
  IAGTQKEASTGKGRGTEETPKNTYI
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_176821
• ORF Size: 1965 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_176821.4
• RefSeq Size: 2020 bp
• RefSeq ORF: 1968 bp
• Locus ID: 338322
• UniProt ID: Q86W26
• Cytogenetics: 11p15.4
• Gene Summary: Members of the NALP protein family typically contain a NACHT domain, a NACHT-associated domain (NAD), a C-terminal leucine-rich repeat (LRR) region, and an N-terminal pyrin domain (PYD). The protein encoded by this gene belongs to the NALP protein family despite lacking the LRR region. This protein likely plays a regulatory role in the innate immune system. The protein belongs to the signal-induced multiprotein complex, the inflammasome, that activates the pro-inflammatory caspases, caspase-1 and caspase-5. Other experiments indicate that this gene acts as a multifunctional negative regulator of inflammation and apoptosis. [provided by RefSeq, Jul 2008]