VWC2 (NM_198570) Human Tagged ORF Clone

CAT#: RG211089

VWC2 (tGFP-tagged) - Human von Willebrand factor C domain containing 2 (VWC2)

Product Images

Circular map for RG211089

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: VWC2
• Synonyms: PSST739; UNQ739
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG211089 representing NM_198570
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCAGCTCCACTGCGATGGCAGTTGGCGCGCTCTCCAGTTCCCTCCTGGTCACCTGCTGCCTGATGG
  TGGCTCTGTGCAGTCCGAGCATCCCGCTGGAGAAGCTGGCCCAGGCACCAGAGCAGCCGGGCCAGGAGAA
  GCGTGAGCACGCCTCTCGGGACGGCCCGGGGCGGGTGAACGAGCTCGGGCGCCCGGCGAGGGACGAGGGC
  GGCAGCGGCCGGGACTGGAAGAGCAAGAGCGGCCGTGGGCTCGCCGGCCGTGAGCCGTGGAGCAAGCTGA
  AGCAGGCCTGGGTCTCCCAGGGCGGGGGCGCCAAGGCCGGGGATCTGCAGGTCCGGCCCCGCGGGGACAC
  CCCGCAGGCGGAAGCCCTGGCCGCAGCCGCCCAGGACGCGATTGGCCCGGAACTCGCGCCCACGCCCGAG
  CCACCCGAGGAGTACGTGTACCCGGACTACCGTGGCAAGGGCTGCGTGGACGAGAGCGGCTTCGTGTACG
  CGATCGGGGAGAAGTTCGCGCCGGGCCCCTCGGCCTGCCCGTGCCTGTGCACCGAGGAGGGGCCGCTGTG
  CGCGCAGCCCGAGTGCCCGAGGCTGCACCCGCGCTGCATCCACGTCGACACGAGCCAGTGCTGCCCGCAG
  TGCAAGGAGAGGAAGAACTACTGCGAGTTCCGGGGCAAGACCTATCAGACTTTGGAGGAGTTCGTGGTGT
  CTCCATGCGAGAGGTGTCGCTGTGAAGCCAACGGTGAGGTGCTATGCACAGTGTCAGCGTGTCCCCAGAC
  GGAGTGTGTGGACCCTGTGTACGAGCCTGATCAGTGCTGTCCCATCTGCAAAAATGGTCCAAACTGCTTT
  GCAGAAACCGCGGTGATCCCTGCTGGCAGAGAAGTGAAGACTGACGAGTGCACCATATGCCACTGTACTT
  ATGAGGAAGGCACATGGAGAATCGAGCGGCAGGCCATGTGCACGAGACATGAATGCAGGCAAATG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG211089 representing NM_198570
  Red=Cloning site Green=Tags(s)
  
  MPSSTAMAVGALSSSLLVTCCLMVALCSPSIPLEKLAQAPEQPGQEKREHASRDGPGRVNELGRPARDEG
  GSGRDWKSKSGRGLAGREPWSKLKQAWVSQGGGAKAGDLQVRPRGDTPQAEALAAAAQDAIGPELAPTPE
  PPEEYVYPDYRGKGCVDESGFVYAIGEKFAPGPSACPCLCTEEGPLCAQPECPRLHPRCIHVDTSQCCPQ
  CKERKNYCEFRGKTYQTLEEFVVSPCERCRCEANGEVLCTVSACPQTECVDPVYEPDQCCPICKNGPNCF
  AETAVIPAGREVKTDECTICHCTYEEGTWRIERQAMCTRHECRQM
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_198570
• ORF Size: 975 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198570.5
• RefSeq Size: 1942 bp
• RefSeq ORF: 978 bp
• Locus ID: 375567
• UniProt ID: Q2TAL6
• Cytogenetics: 7p12.2
• Gene Summary: This gene encodes a secreted bone morphogenic protein antagonist. The encoded protein is possibly involved in neural function and development and may have a role in cell adhesion.[provided by RefSeq, Oct 2009]