ZNF182 (NM_001007088) Human Tagged ORF Clone

CAT#: RG211064

ZNF182 (tGFP-tagged) - Human zinc finger protein 182 (ZNF182), transcript variant 2

Product Images

Circular map for RG211064

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ZNF182
• Synonyms: HHZ150; KOX14; Zfp182; ZNF21
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG211064 representing NM_001007088
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAAACCCCAGGGGCTAGTGACATTTGAAGATGTAGCTGTGGATTTCACCCAGGAGGAGTGGCAGT
  ACCTGAACCCACCACAGAGGACCCTGTACAGAGACGTGATGCTGGAGACCTACAGCAACTTGGTCTTTGT
  GGGGCAGCAGGTTACCAAACCAAACCTCATCCTCAAGTTGGAGGTAGAAGAATGCCCGGCAGAAGGAAAA
  ATCCCATTTTGGAACTTTCCAGAAGTCTGTCAAGTTGATGAACAGATTGAGAGGCAACATCAGGATGACC
  AAGATAAATGTCTGCTGATGCAAGTTGGATTTTCTGACAAGAAAACAATTATCACCAAGAGTGCTCGTGA
  CTGTCATGAGTTTGGAAACATACTTCATCTGAGTACAAACCTTGTTGCTTCAATACAAAGACCCGATAAA
  CACGAATCATTTGGAAATAATATGGTAGATAATTTAGACTTATTTAGTAGAAGTTCTGCAGAAAATAAAT
  ATGATAATGGATGTGCAAAATTGTTCTTCCATACTGAGTATGAGAAAACAAATCCTGGAATGAAGCCCTA
  TGGCTATAAAGAGTGTGGGAAAGGTCTTAGGCGAAAGAAAGGCCTTAGTCTACATCAGAGAATTAAAAAT
  GGAGAGAAACCCTTTGAATGTACTGCATGTAGGAAAACCTTCAGCAAGAAGTCACACCTCATTGTACATT
  GGAGAACTCATACGGGAGAGAAACCTTTTGGATGTACCGAATGTGGAAAAGCTTTTAGCCAAAAATCTCA
  GCTCATTATACACTTGCGAACTCATACAGGAGAGAGACCCTTTGAGTGTCCTGAATGTGGAAAAGCCTTC
  AGAGAAAAGTCAACTGTCATCATACATTACAGGACTCACACAGGAGAAAAACCTTATGAATGTAATGAAT
  GTGGAAAAGCCTTCACTCAGAAGTCCAACCTCATTGTCCATCAGAAAACCCACACTGGAGAGAAAACCTA
  TGAATGCACTAAATGTGGAGAATCTTTCATACAGAAGCTTGATCTAATTATACATCATAGTACCCATACA
  GGAAAGAAACCCCATGAATGTAATGAGTGTAAGAAAACTTTCAGTGATAAGTCAACTCTCATTATACACC
  AGAGAACTCATACGGGAGAGAAACCTCATAAATGTACTGAATGTGGGAAGTCTTTCAATGAGAAGTCAAC
  CCTCATTGTGCATCAAAGAACTCATACAGGAGAGAAACCCTATGAATGTGATGTGTGTGGAAAAACCTTC
  ACGCAAAAGTCAAACCTTGGTGTACATCAGAGAACTCATTCAGGAGAGAAACCCTTTGAATGTAATGAAT
  GTGAGAAAGCCTTCTCTCAGAAGTCCTACCTCATGCTGCATCAGAGAGGTCATACAGGAGAGAAACCTTA
  CGAGTGCAATGAATGTGAAAAAGCATTTTCACAGAAATCATATCTCATTATACATCAAAGAACTCATACA
  GAAGAAAAACCCTATAAATGTAATGAATGTGGCAAAGCCTTCAGAGAAAAGTCAAAGCTCATTATACATC
  AGAGAATTCATACAGGAGAGAAACCTTATGAATGTCCTGTGTGTTGGAAAGCTTTTAGCCAGAAGTCACA
  GCTCATAATACATCAGAGAACGCACACGGGAGAGAAACCCTATGCATGCACTGAGTGTGGCAAAGCCTTC
  CGAGAAAAGTCAACATTCACTGTACATCAAAGAACTCATACTGGAGAGAAACCCTATAAATGTACAGAAT
  GTGGGAAAGCCTTTACCCAAAAATCAAACCTTATTGTACATCAGCGAACCCATGCAGGAAAGAAAGCCCA
  TGGAAGAGGCCACACTCGGAAGTCAAAGTTCATGGCACAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG211064 representing NM_001007088
  Red=Cloning site Green=Tags(s)
  
  MAKPQGLVTFEDVAVDFTQEEWQYLNPPQRTLYRDVMLETYSNLVFVGQQVTKPNLILKLEVEECPAEGK
  IPFWNFPEVCQVDEQIERQHQDDQDKCLLMQVGFSDKKTIITKSARDCHEFGNILHLSTNLVASIQRPDK
  HESFGNNMVDNLDLFSRSSAENKYDNGCAKLFFHTEYEKTNPGMKPYGYKECGKGLRRKKGLSLHQRIKN
  GEKPFECTACRKTFSKKSHLIVHWRTHTGEKPFGCTECGKAFSQKSQLIIHLRTHTGERPFECPECGKAF
  REKSTVIIHYRTHTGEKPYECNECGKAFTQKSNLIVHQKTHTGEKTYECTKCGESFIQKLDLIIHHSTHT
  GKKPHECNECKKTFSDKSTLIIHQRTHTGEKPHKCTECGKSFNEKSTLIVHQRTHTGEKPYECDVCGKTF
  TQKSNLGVHQRTHSGEKPFECNECEKAFSQKSYLMLHQRGHTGEKPYECNECEKAFSQKSYLIIHQRTHT
  EEKPYKCNECGKAFREKSKLIIHQRIHTGEKPYECPVCWKAFSQKSQLIIHQRTHTGEKPYACTECGKAF
  REKSTFTVHQRTHTGEKPYKCTECGKAFTQKSNLIVHQRTHAGKKAHGRGHTRKSKFMAH
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001007088
• ORF Size: 1860 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001007088.2
• RefSeq Size: 3504 bp
• RefSeq ORF: 1863 bp
• Locus ID: 7569
• UniProt ID: P17025
• Cytogenetics: Xp11.23
• Gene Summary: Zinc-finger proteins bind nucleic acids and play important roles in various cellular functions, including cell proliferation, differentiation, and apoptosis. This gene encodes a zinc finger protein, and belongs to the krueppel C2H2-type zinc-finger protein family. It may be involved in transcriptional regulation. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, May 2010]