CREB3L3 (NM_032607) Human Tagged ORF Clone

CAT#: RG210921

CREB3L3 (tGFP-tagged) - Human cAMP responsive element binding protein 3-like 3 (CREB3L3)

Product Images

Circular map for RG210921

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CREB3L3
• Synonyms: CREB-H; CREBH; HYST1481; HYTG2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG210921 representing NM_032607
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATACGGATTTAGCTGCTGGAAAGATGGCTTCTGCTGCCTGCTCCATGGACCCCATCGACAGCTTTG
  AGCTCCTGGATCTCCTGTTTGACCGGCAGGACGGCATCCTGAGACACGTGGAGCTGGGCGAGGGCTGGGG
  TCACGTCAAGGACCAGCAGGTCCTGCCAAACCCCGACTCTGACGACTTCCTCAGCTCCATCCTGGGCTCT
  GGAGACTCACTGCCCAGCTCCCCACTCTGGTCCCCCGAAGGCAGTGATAGTGGCATCTCCGAAGACCTCC
  CCTCCGACCCCCAGGACACCCCTCCACGCAGCGGACCAGCCACCTCCCCCGCCGGCTGCCATCCTGCCCA
  GCCTGGCAAGGGGCCCTGCCTCTCCTATCATCCTGGCAACTCTTGCTCCACCACAACCCCAGGGCCAGTG
  ATCCAAGTACCTGAAGCCTCTGTGACCATAGACCTGGAAATGTGGAGCCCAGGAGGAAGGATCTGTGCTG
  AGAAGCCGGCTGATCCGGTGGACCTGTCCCCACGATGCAATCTCACCGTGAAAGACCTCCTCCTTTCGGG
  CAGCAGTGGGGACCTGCAACAGCATCACCTGGGGGCCTCCTACCTCCTGCGACCTGGGGCTGGGCACTGT
  CAGGAGCTGGTGCTCACCGAGGATGAGAAGAAGCTGCTGGCTAAAGAAGGCATCACCCTGCCCACTCAGC
  TGCCCCTCACTAAGTACGAGGAGCGAGTGCTGAAAAAAATCCGCCGGAAAATCCGGAACAAGCAGTCGGC
  GCAAGAAAGCAGGAAGAAGAAGAAGGAATATATCGATGGCCTGGAGACTCGGATGTCAGCTTGCACTGCT
  CAGAATCAGGAGTTACAGAGGAAAGTCTTGCATCTCGAGAAGCAAAACCTGTCCCTCTTGGAGCAACTGA
  AGAAACTCCAGGCCATTGTGGTGCAGTCCACCAGCAAGTCAGCCCAGACAGGCACCTGTGTCGCAGTCCT
  GTTGCTGTCCTTTGCCCTCATCATCCTCCCCTCCATCAGCCCTTTTGGCCCCAACAAAACCGAGAGCCCT
  GGGGACTTTGCGCCTGTACGAGTGTTCTCCAGAACTTTGCACAACGATGCTGCCTCCCGCGTGGCTGCTG
  ATGCTGTGCCAGGCTCCGAGGCCCCAGGACCCCGACCCGAGGCTGACACAACCCGAGAAGAGTCTCCAGG
  AAGCCCCGGGGCAGACTGGGGCTTCCAGGACACCGCGAACCTGACCAATTCGACGGAGGAGCTGGACAAC
  GCCACCCTGGTCCTGAGGAATGCAACAGAGGGGCTGGGCCAGGTCGCCCTGCTGGACTGGGTGGCGCCTG
  GGCCGAGCACTGGCTCAGGACGTGCAGGGCTGGAGGCGGCGGGAGACGAGCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG210921 representing NM_032607
  Red=Cloning site Green=Tags(s)
  
  MNTDLAAGKMASAACSMDPIDSFELLDLLFDRQDGILRHVELGEGWGHVKDQQVLPNPDSDDFLSSILGS
  GDSLPSSPLWSPEGSDSGISEDLPSDPQDTPPRSGPATSPAGCHPAQPGKGPCLSYHPGNSCSTTTPGPV
  IQVPEASVTIDLEMWSPGGRICAEKPADPVDLSPRCNLTVKDLLLSGSSGDLQQHHLGASYLLRPGAGHC
  QELVLTEDEKKLLAKEGITLPTQLPLTKYEERVLKKIRRKIRNKQSAQESRKKKKEYIDGLETRMSACTA
  QNQELQRKVLHLEKQNLSLLEQLKKLQAIVVQSTSKSAQTGTCVAVLLLSFALIILPSISPFGPNKTESP
  GDFAPVRVFSRTLHNDAASRVAADAVPGSEAPGPRPEADTTREESPGSPGADWGFQDTANLTNSTEELDN
  ATLVLRNATEGLGQVALLDWVAPGPSTGSGRAGLEAAGDEL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032607
• ORF Size: 1383 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032607.3
• RefSeq Size: 2586 bp
• RefSeq ORF: 1386 bp
• Locus ID: 84699
• UniProt ID: Q68CJ9
• Cytogenetics: 19p13.3
• Protein Families: Transcription Factors
• Protein Pathways: Huntington's disease, Melanogenesis, Prostate cancer
• Gene Summary: This gene encodes a member of the basic-leucine zipper family and the AMP-dependent transcription factor family. The encoded protein is localized to the endoplasmic reticulum and acts as a transcription factor activated by cyclic AMP stimulation. The encoded protein binds the cyclic AMP response element (CRE) and the box-B element and has been linked to acute inflammatory response, hepatocellular carcinoma, triglyceride metabolism, and hepcidin expression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2012]