Gastric Inhibitory Polypeptide Receptor (GIPR) (NM_000164) Human Tagged ORF Clone

CAT#: RG210811

GIPR (tGFP-tagged) - Human gastric inhibitory polypeptide receptor (GIPR)

Product Images

Circular map for RG210811

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Gastric Inhibitory Polypeptide Receptor
• Synonyms: PGQTL2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG210811 representing NM_000164
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTACCTCTCCGATCCTGCAGCTGCTGCTGCGGCTCTCACTGTGCGGGCTGCTGCTCCAGAGGGCGG
  AGACAGGCTCTAAGGGGCAGACGGCGGGGGAGCTGTACCAGCGCTGGGAACGGTACCGCAGGGAGTGCCA
  GGAGACCTTGGCAGCCGCGGAACCGCCTTCAGGCCTCGCCTGTAACGGGTCCTTCGATATGTACGTCTGC
  TGGGACTATGCTGCACCCAATGCCACTGCCCGTGCGTCCTGCCCCTGGTACCTGCCCTGGCACCACCATG
  TGGCTGCAGGTTTCGTCCTCCGCCAGTGTGGCAGTGATGGCCAATGGGGACTTTGGAGAGACCATACACA
  ATGTGAGAACCCAGAGAAGAATGAGGCCTTTCTGGACCAAAGGCTCATCTTGGAGCGGTTGCAGGTCATG
  TACACTGTCGGCTACTCCCTGTCTCTCGCCACACTGCTGCTAGCCCTGCTCATCTTGAGTTTGTTCAGGC
  GGCTACATTGCACTAGAAACTATATCCACATCAACCTGTTCACGTCTTTCATGCTGCGAGCTGCGGCCAT
  TCTCAGCCGAGACCGTCTGCTACCTCGACCTGGCCCCTACCTTGGGGACCAGGCCCTTGCGCTGTGGAAC
  CAGGCCCTCGCTGCCTGCCGCACGGCCCAGATCGTGACCCAGTACTGCGTGGGTGCCAACTACACGTGGC
  TGCTGGTGGAGGGCGTCTACCTGCACAGTCTCCTGGTGCTCGTGGGAGGCTCCGAGGAGGGCCACTTCCG
  CTACTACCTGCTCCTCGGCTGGGGGGCCCCCGCGCTTTTCGTCATTCCCTGGGTGATCGTCAGGTACCTG
  TACGAGAACACGCAGTGCTGGGAGCGCAACGAAGTCAAGGCCATTTGGTGGATTATACGGACCCCCATCC
  TCATGACCATCTTGATTAATTTCCTCATTTTTATCCGCATTCTTGGCATTCTCCTGTCCAAGCTGAGGAC
  ACGGCAAATGCGCTGCCGGGATTACCGGCTGAGGCTGGCTCGCTCCACGCTGACGCTGGTGCCCCTGCTG
  GGTGTCCACGAGGTGGTGTTTGCTCCCGTGACAGAGGAACAGGCCCGGGGCGCCCTGCGCTTCGCCAAGC
  TCGGCTTTGAGATCTTCCTCAGCTCCTTCCAGGGCTTCCTGGTCAGCGTCCTCTACTGCTTCATCAACAA
  GGAGGTGCAGTCGGAGATCCGCCGTGGCTGGCACCACTGCCGCCTGCGCCGCAGCCTGGGCGAGGAGCAA
  CGCCAGCTCCCGGAGCGCGCCTTCCGGGCCCTGCCCTCCGGCTCCGGCCCGGGCGAGGTCCCCACCAGCC
  GCGGCTTGTCCTCGGGGACCCTCCCAGGGCCTGGGAATGAGGCCAGCCGGGAGTTGGAAAGTTACTGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG210811 representing NM_000164
  Red=Cloning site Green=Tags(s)
  
  MTTSPILQLLLRLSLCGLLLQRAETGSKGQTAGELYQRWERYRRECQETLAAAEPPSGLACNGSFDMYVC
  WDYAAPNATARASCPWYLPWHHHVAAGFVLRQCGSDGQWGLWRDHTQCENPEKNEAFLDQRLILERLQVM
  YTVGYSLSLATLLLALLILSLFRRLHCTRNYIHINLFTSFMLRAAAILSRDRLLPRPGPYLGDQALALWN
  QALAACRTAQIVTQYCVGANYTWLLVEGVYLHSLLVLVGGSEEGHFRYYLLLGWGAPALFVIPWVIVRYL
  YENTQCWERNEVKAIWWIIRTPILMTILINFLIFIRILGILLSKLRTRQMRCRDYRLRLARSTLTLVPLL
  GVHEVVFAPVTEEQARGALRFAKLGFEIFLSSFQGFLVSVLYCFINKEVQSEIRRGWHHCRLRRSLGEEQ
  RQLPERAFRALPSGSGPGEVPTSRGLSSGTLPGPGNEASRELESYC
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000164
• ORF Size: 1398 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_000164.4
• RefSeq Size: 2024 bp
• RefSeq ORF: 1401 bp
• Locus ID: 2696
• UniProt ID: P48546
• Cytogenetics: 19q13.32
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• Gene Summary: This gene encodes a G-protein coupled receptor for gastric inhibitory polypeptide (GIP), which was originally identified as an activity in gut extracts that inhibited gastric acid secretion and gastrin release, but subsequently was demonstrated to stimulate insulin release in the presence of elevated glucose. Mice lacking this gene exhibit higher blood glucose levels with impaired initial insulin response after oral glucose load. Defect in this gene thus may contribute to the pathogenesis of diabetes. [provided by RefSeq, Oct 2011]