FBXO2 (NM_012168) Human Tagged ORF Clone

CAT#: RG210500

FBXO2 (tGFP-tagged) - Human F-box protein 2 (FBXO2)

Product Images

Circular map for RG210500

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: FBXO2
• Synonyms: FBG1; Fbs1; FBX2; NFB42; OCP1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG210500 representing NM_012168
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGGAGACGGTGACCCAGAGAGCGTGGGCCAGCCCGAGGAGGCAAGCCCGGAGGAGCAGCCAGAGG
  AGGCGAGTGCTGAGGAGGAGCGGCCGGAGGACCAGCAGGAGGAGGAGGCGGCGGCCGCCGCCGCGTACCT
  GGACGAGCTGCCCGAGCCGCTGCTGCTGCGCGTGCTGGCCGCACTGCCGGCCGCCGAGCTGGTGCAGGCC
  TGCCGCCTGGTGTGCCTGCGCTGGAAGGAGCTGGTGGACGGCGCCCCGCTGTGGCTGCTCAAGTGCCAGC
  AGGAGGGGCTGGTGCCCGAGGGCGGCGTGGAGGAGGAGCGCGACCACTGGCAGCAGTTCTACTTCCTGAG
  CAAGCGGCGCCGCAACCTTCTGCGTAACCCGTGTGGGGAAGAGGACTTGGAAGGCTGGTGTGACGTGGAG
  CATGGTGGGGACGGCTGGAGGGTGGAGGAGCTGCCTGGAGACAGTGGGGTGGAGTTCACCCACGATGAGA
  GCGTCAAGAAGTACTTCGCCTCCTCCTTTGAGTGGTGTCGCAAAGCACAGGTCATTGACCTGCAGGCTGA
  GGGCTACTGGGAGGAGCTGCTGGACACGACTCAGCCGGCCATCGTGGTGAAGGACTGGTACTCGGGCCGC
  AGCGACGCTGGTTGCCTCTACGAGCTCACCGTTAAGCTACTGTCCGAGCACGAGAACGTGCTGGCTGAGT
  TCAGCAGCGGGCAGGTGGCAGTGCCCCAAGACAGTGACGGCGGGGGCTGGATGGAGATCTCCCACACCTT
  CACCGACTACGGGCCGGGCGTCCGCTTCGTCCGCTTCGAGCACGGGGGGCAGGACTCCGTCTACTGGAAG
  GGCTGGTTCGGGGCCCGGGTGACCAACAGCAGCGTGTGGGTAGAACCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG210500 representing NM_012168
  Red=Cloning site Green=Tags(s)
  
  MDGDGDPESVGQPEEASPEEQPEEASAEEERPEDQQEEEAAAAAAYLDELPEPLLLRVLAALPAAELVQA
  CRLVCLRWKELVDGAPLWLLKCQQEGLVPEGGVEEERDHWQQFYFLSKRRRNLLRNPCGEEDLEGWCDVE
  HGGDGWRVEELPGDSGVEFTHDESVKKYFASSFEWCRKAQVIDLQAEGYWEELLDTTQPAIVVKDWYSGR
  SDAGCLYELTVKLLSEHENVLAEFSSGQVAVPQDSDGGGWMEISHTFTDYGPGVRFVRFEHGGQDSVYWK
  GWFGARVTNSSVWVEP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012168
• ORF Size: 888 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012168.6
• RefSeq Size: 1551 bp
• RefSeq ORF: 891 bp
• Locus ID: 26232
• UniProt ID: Q9UK22
• Cytogenetics: 1p36.22
• Domains: F-box, FBA
• Protein Families: Druggable Genome
• Protein Pathways: Ubiquitin mediated proteolysis
• Gene Summary: This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. This protein is highly similar to the rat NFB42 (neural F Box 42 kDa) protein which is enriched in the nervous system and may play a role in maintaining neurons in a postmitotic state. [provided by RefSeq, Jul 2008]