GRPR (NM_005314) Human Tagged ORF Clone

CAT#: RG210362

  • TrueORF®

GRPR (tGFP-tagged) - Human gastrin-releasing peptide receptor (GRPR)

ORF Plasmid: DDK tGFP

Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro

AAV Particle: DDK


  "NM_005314" in other vectors (6)

Reconstitution Protocol

USD 657.00

In Stock*

Size
    • 10 ug

Product Images

Frequently bought together (5)
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Mouse monoclonal turboGFP antibody, clone OTI2H8
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DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
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Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
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GRPR Rabbit Polyclonal (Extracellular Domain) Antibody
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Other products for "GRPR"

Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Symbol GRPR
Synonyms BB2; BB2R; BRS2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG210362 representing NM_005314
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTCTAAATGACTGTTTCCTTCTGAACTTGGAGGTGGACCATTTCATGCACTGCAACATCTCCAGTC
ACAGTGCGGATCTCCCCGTGAACGATGACTGGTCCCACCCGGGGATCCTCTATGTCATCCCTGCAGTTTA
TGGGGTTATCATTCTGATAGGCCTCATTGGCAACATCACTTTGATCAAGATCTTCTGTACAGTCAAGTCC
ATGCGAAACGTTCCAAACCTGTTCATTTCCAGTCTGGCTTTGGGAGACCTGCTCCTCCTAATAACGTGTG
CTCCAGTGGATGCCAGCAGGTACCTGGCTGACAGATGGCTATTTGGCAGGATTGGCTGCAAACTGATCCC
CTTTATACAGCTTACCTCTGTTGGGGTGTCTGTCTTCACACTCACGGCGCTCTCGGCAGACAGATACAAA
GCCATTGTCCGGCCAATGGATATCCAGGCCTCCCATGCCCTGATGAAGATCTGCCTCAAAGCCGCCTTTA
TCTGGATCATCTCCATGCTGCTGGCCATTCCAGAGGCCGTGTTTTCTGACCTCCATCCCTTCCATGAGGA
AAGCACCAACCAGACCTTCATTAGCTGTGCCCCATACCCACACTCTAATGAGCTTCACCCCAAAATCCAT
TCTATGGCTTCCTTTCTGGTCTTCTACGTCATCCCACTGTCGATCATCTCTGTTTACTACTACTTCATTG
CTAAAAATCTGATCCAGAGTGCTTACAATCTTCCCGTGGAAGGGAATATACATGTCAAGAAGCAGATTGA
ATCCCGGAAGCGACTTGCCAAGACAGTGCTGGTGTTTGTGGGCCTGTTCGCCTTCTGCTGGCTCCCCAAT
CATGTCATCTACCTGTACCGCTCCTACCACTACTCTGAGGTGGACACCTCCATGCTCCACTTTGTCACCA
GCATCTGTGCCCGCCTCCTGGCCTTCACCAACTCCTGCGTGAACCCCTTTGCCCTCTACCTGCTGAGCAA
GAGTTTCAGGAAACAGTTCAACACTCAGCTGCTCTGTTGCCAGCCTGGCCTGATCATCCGGTCTCACAGC
ACTGGAAGGAGTACAACCTGCATGACCTCCCTCAAGAGTACCAACCCCTCCGTGGCCACCTTTAGCCTCA
TCAATGGAAACATCTGTCACGAGCGGTATGTC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG210362 representing NM_005314
Red=Cloning site Green=Tags(s)

MALNDCFLLNLEVDHFMHCNISSHSADLPVNDDWSHPGILYVIPAVYGVIILIGLIGNITLIKIFCTVKS
MRNVPNLFISSLALGDLLLLITCAPVDASRYLADRWLFGRIGCKLIPFIQLTSVGVSVFTLTALSADRYK
AIVRPMDIQASHALMKICLKAAFIWIISMLLAIPEAVFSDLHPFHEESTNQTFISCAPYPHSNELHPKIH
SMASFLVFYVIPLSIISVYYYFIAKNLIQSAYNLPVEGNIHVKKQIESRKRLAKTVLVFVGLFAFCWLPN
HVIYLYRSYHYSEVDTSMLHFVTSICARLLAFTNSCVNPFALYLLSKSFRKQFNTQLLCCQPGLIIRSHS
TGRSTTCMTSLKSTNPSVATFSLINGNICHERYV

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_005314
ORF Size 1152 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_005314.1
RefSeq Size 2681 bp
RefSeq ORF 1155 bp
Locus ID 2925
UniProt ID P30550
Cytogenetics Xp22.2
Protein Families Druggable Genome, GPCR, Transmembrane
Protein Pathways Calcium signaling pathway, Neuroactive ligand-receptor interaction
Gene Summary Gastrin-releasing peptide (GRP) regulates numerous functions of the gastrointestinal and central nervous systems, including release of gastrointestinal hormones, smooth muscle cell contraction, and epithelial cell proliferation and is a potent mitogen for neoplastic tissues. The effects of GRP are mediated through the gastrin-releasing peptide receptor. This receptor is a glycosylated, 7-transmembrane G-protein coupled receptor that activates the phospholipase C signaling pathway. The receptor is aberrantly expressed in numerous cancers such as those of the lung, colon, and prostate. An individual with autism and multiple exostoses was found to have a balanced translocation between chromosome 8 and a chromosome X breakpoint located within the gastrin-releasing peptide receptor gene. [provided by RefSeq, Jul 2008]

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