PON1 (NM_000446) Human Tagged ORF Clone

CAT#: RG210356

PON1 (tGFP-tagged) - Human paraoxonase 1 (PON1)

Product Images

Circular map for RG210356

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PON1
• Synonyms: ESA; MVCD5; PON
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG210356 representing NM_000446
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGAAGCTGATTGCGCTCACCCTCTTGGGGATGGGACTGGCACTCTTCAGGAACCACCAGTCTTCTT
  ACCAAACACGACTTAATGCTCTCCGAGAGGTACAACCCGTAGAACTTCCTAACTGTAATTTAGTTAAAGG
  AATCGAAACTGGCTCTGAAGACTTGGAGATACTGCCTAATGGACTGGCTTTCATTAGCTCTGGATTAAAG
  TATCCTGGAATAAAGAGCTTCAACCCCAACAGTCCTGGAAAAATACTTCTGATGGACCTGAATGAAGAAG
  ATCCAACAGTGTTGGAATTGGGGATCACTGGAAGTAAATTTGATGTATCTTCATTTAACCCTCATGGGAT
  TAGCACATTCACAGATGAAGATAATGCCATGTACCTCCTGGTGGTGAACCATCCAGATGCCAAGTCCACA
  GTGGAGTTGTTTAAATTTCAAGAAGAAGAAAAATCGCTTTTGCATCTAAAAACCATCAGACATAAACTTC
  TGCCTAATTTGAATGATATTGTTGCTGTGGGACCTGAGCACTTTTATGGCACAAATGATCACTATTTTCT
  TGACCCCTACTTACAATCCTGGGAGATGTATTTGGGTTTAGCGTGGTCGTATGTTGTCTACTATAGTCCA
  AGTGAAGTTCGAGTGGTGGCAGAAGGATTTGATTTTGCTAATGGAATCAACATTTCACCCGATGGCAAGT
  ATGTCTATATAGCTGAGTTGCTGGCTCATAAGATTCATGTGTATGAAAAGCATGCTAATTGGACTTTAAC
  TCCATTGAAGTCCCTTGACTTTAATACCCTCGTGGATAACATATCTGTGGATCCTGAGACAGGAGACCTT
  TGGGTTGGATGCCATCCCAATGGCATGAAAATCTTCTTCTATGACTCAGAGAATCCTCCTGCATCAGAGG
  TGCTTCGAATCCAGAACATTCTAACAGAAGAACCTAAAGTGACACAGGTTTATGCAGAAAATGGCACAGT
  GTTGCAAGGCAGTACAGTTGCCTCTGTGTACAAAGGGAAACTGCTGATTGGCACAGTGTTTCACAAAGCT
  CTTTACTGTGAGCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG210356 representing NM_000446
  Red=Cloning site Green=Tags(s)
  
  MAKLIALTLLGMGLALFRNHQSSYQTRLNALREVQPVELPNCNLVKGIETGSEDLEILPNGLAFISSGLK
  YPGIKSFNPNSPGKILLMDLNEEDPTVLELGITGSKFDVSSFNPHGISTFTDEDNAMYLLVVNHPDAKST
  VELFKFQEEEKSLLHLKTIRHKLLPNLNDIVAVGPEHFYGTNDHYFLDPYLQSWEMYLGLAWSYVVYYSP
  SEVRVVAEGFDFANGINISPDGKYVYIAELLAHKIHVYEKHANWTLTPLKSLDFNTLVDNISVDPETGDL
  WVGCHPNGMKIFFYDSENPPASEVLRIQNILTEEPKVTQVYAENGTVLQGSTVASVYKGKLLIGTVFHKA
  LYCEL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000446
• ORF Size: 1065 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000446.7
• RefSeq Size: 2395 bp
• RefSeq ORF: 1068 bp
• Locus ID: 5444
• UniProt ID: P27169
• Cytogenetics: 7q21.3
• Domains: Arylesterase
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Metabolic pathways
• Gene Summary: This gene encodes a member of the paraoxonase family of enzymes and exhibits lactonase and ester hydrolase activity. Following synthesis in the kidney and liver, the enzyme is secreted into the circulation, where it binds to high density lipoprotein (HDL) particles and hydrolyzes thiolactones and xenobiotics, including paraoxon, a metabolite of the insecticide parathion. Polymorphisms in this gene may be associated with coronary artery disease and diabetic retinopathy. The gene is found in a cluster of three related paraoxonase genes on chromosome 7. [provided by RefSeq, Aug 2017]