FAAH1 (FAAH) (NM_001441) Human Tagged ORF Clone

CAT#: RG210331

FAAH (tGFP-tagged) - Human fatty acid amide hydrolase (FAAH)

Product Images

Circular map for RG210331

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: FAAH1
• Synonyms: FAAH-1; PSAB
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG210331 representing NM_001441
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCAGTACGAGCTGTGGGCCGCGCTGCCTGGCGCCTCCGGGGTCGCCCTGGCCTGCTGCTTCGTGG
  CGGCGGCCGTGGCCCTGCGCTGGTCCGGGCGCCGGACGGCGCGGGGCGCGGTGGTCCGGGCGCGACAGAG
  GCAGCGAGCGGGCCTGGAGAACATGGACAGGGCGGCGCAGCGCTTCCGGCTCCAGAACCCAGACCTGGAC
  TCAGAGGCGCTGCTAGCCCTGCCCCTGCCTCAGCTGGTGCAGAAGTTACACAGTAGAGAGCTGGCCCCTG
  AGGCCGTGCTCTTCACCTATGTGGGAAAGGCCTGGGAAGTGAACAAAGGGACCAACTGTGTGACCTCCTA
  TCTGGCTGACTGTGAGACTCAGCTGTCTCAGGCCCCAAGGCAGGGCCTGCTCTATGGCGTCCCTGTGAGC
  CTCAAGGAGTGCTTCACCTACAAGGGCCAGGACTCCACGCTGGGCTTGAGCCTGAATGAAGGGGTGCCGG
  CGGAGTGCGACAGCGTAGTGGTGCATGTGCTGAAGCTGCAGGGTGCCGTGCCCTTCGTGCACACCAATGT
  TCCACAGTCCATGTTCAGCTATGACTGCAGTAACCCCCTCTTTGGCCAGACCGTGAACCCATGGAAGTCC
  TCCAAAAGCCCAGGGGGCTCCTCAGGGGGTGAAGGGGCCCTCATCGGGTCTGGAGGCTCCCCCCTGGGCT
  TAGGCACTGATATCGGAGGCAGCATCCGCTTCCCCTCCTCCTTCTGCGGCATCTGCGGCCTCAAGCCCAC
  AGGGAACCGCCTCAGCAAGAGTGGCCTGAAGGGCTGTGTCTATGGACAGGAGGCAGTGCGTCTCTCCGTG
  GGCCCCATGGCCCGGGACGTGGAGAGCCTGGCACTGTGCCTGCGAGCCCTGCTGTGCGAGGACATGTTCC
  GCTTGGACCCCACTGTGCCTCCCTTGCCCTTCAGAGAAGAGGTCTACACCAGCTCTCAGCCCCTGCGTGT
  GGGGTACTATGAGACTGACAACTATACCATGCCCTCCCCGGCCATGAGGCGGGCCGTGCTGGAGACCAAA
  CAGAGCCTTGAGGCTGCGGGGCACACGCTGGTTCCCTTCTTGCCAAGCAACATACCCCATGCTCTGGAGA
  CCCTGTCAACAGGTGGGCTCTTCAGTGATGGTGGCCACACCTTCCTACAGAACTTCAAAGGTGATTTCGT
  GGACCCCTGCCTGGGGGACCTGGTCTCAATTCTGAAGCTTCCCCAATGGCTTAAAGGACTGCTGGCCTTC
  CTGGTGAAGCCTCTGCTGCCAAGGCTGTCAGCTTTCCTCAGCAACATGAAGTCTCGTTCGGCTGGAAAAC
  TCTGGGAACTGCAGCACGAGATCGAGGTGTACCGCAAAACCGTGATTGCCCAGTGGAGGGCGCTGGACCT
  GGATGTGGTGCTGACCCCCATGCTGGCCCCTGCTCTGGACTTGAATGCCCCAGGCAGGGCCACAGGGGCC
  GTCAGCTACACTATGCTGTACAACTGCCTGGACTTCCCTGCAGGGGTGGTGCCTGTCACCACGGTGACTG
  CTGAGGACGAGGCCCAGATGGAACATTACAGGGGCTACTTTGGGGATATCTGGGACAAGATGCTGCAGAA
  GGGCATGAAGAAGAGTGTGGGGCTGCCGGTGGCCGTGCAGTGTGTGGCTCTGCCCTGGCAAGAAGAGTTG
  TGTCTGCGGTTCATGCGGGAGGTGGAGCGACTGATGACCCCTGAAAAGCAGTCATCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG210331 representing NM_001441
  Red=Cloning site Green=Tags(s)
  
  MVQYELWAALPGASGVALACCFVAAAVALRWSGRRTARGAVVRARQRQRAGLENMDRAAQRFRLQNPDLD
  SEALLALPLPQLVQKLHSRELAPEAVLFTYVGKAWEVNKGTNCVTSYLADCETQLSQAPRQGLLYGVPVS
  LKECFTYKGQDSTLGLSLNEGVPAECDSVVVHVLKLQGAVPFVHTNVPQSMFSYDCSNPLFGQTVNPWKS
  SKSPGGSSGGEGALIGSGGSPLGLGTDIGGSIRFPSSFCGICGLKPTGNRLSKSGLKGCVYGQEAVRLSV
  GPMARDVESLALCLRALLCEDMFRLDPTVPPLPFREEVYTSSQPLRVGYYETDNYTMPSPAMRRAVLETK
  QSLEAAGHTLVPFLPSNIPHALETLSTGGLFSDGGHTFLQNFKGDFVDPCLGDLVSILKLPQWLKGLLAF
  LVKPLLPRLSAFLSNMKSRSAGKLWELQHEIEVYRKTVIAQWRALDLDVVLTPMLAPALDLNAPGRATGA
  VSYTMLYNCLDFPAGVVPVTTVTAEDEAQMEHYRGYFGDIWDKMLQKGMKKSVGLPVAVQCVALPWQEEL
  CLRFMREVERLMTPEKQSS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001441
• ORF Size: 1737 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001441.2
• RefSeq Size: 2063 bp
• RefSeq ORF: 1740 bp
• Locus ID: 2166
• UniProt ID: O00519
• Cytogenetics: 1p33
• Domains: Amidase
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: This gene encodes a protein that is responsible for the hydrolysis of a number of primary and secondary fatty acid amides, including the neuromodulatory compounds anandamide and oleamide. [provided by RefSeq, Jul 2008]