SLC38A6 (NM_153811) Human Tagged ORF Clone

CAT#: RG210307

SLC38A6 (tGFP-tagged) - Human solute carrier family 38, member 6 (SLC38A6), transcript variant 2

Product Images

Circular map for RG210307

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SLC38A6
• Synonyms: NAT-1; SNAT6
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG210307 representing NM_153811
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCGTCCTGGGGGAGCTTCAACGCTGAGCGGGGCTGGTATGTCTCTGTCCAGCAGCCTGAAGAAG
  CGGAGGCCGAAGAGTTGAGTCCGTTGCTAAGCAACGAACTTCACAGACAGCGATCCCCAGGTGTTTCATT
  TGGTTTATCAGTGTTTAATTTGATGAATGCCATCATGGGAAGTGGCATCCTTGGCTTAGCTTATGTTATG
  GCTAATACCGGTGTCTTTGGATTTAGCTTCTTGCTGCTGACAGTTGCTCTCCTGGCTTCTTACTCAGTCC
  ATCTTCTGCTTAGTATGTGTATTCAGACAGCTGTAACATCTTATGAAGATCTTGGACTCTTTGCATTTGG
  ATTACCTGGAAAGTTGGTGGTGGCAGGCACCATAATAATTCAGAATATTGGAGCTATGTCATCTTATCTT
  TTAATTATTAAAACAGAGCTTCCTGCTGCTATTGCAGAATTTTTGACTGGAGACTATAGTAGATATTGGT
  ATCTTGATGGACAAACACTACTAATAATCATATGTGTTGGCATTGTGTTCCCTCTTGCACTTCTTCCCAA
  AATAGGCTTTCTTGGCTACACAAGTAGTTTATCATTTTTCTTTATGATGTTCTTTGCTCTTGTGGTAATA
  ATTAAAAAATGGTCCATCCCTTGTCCTCTGACATTAAATTATGTAGAGAAAGGCTTCCAGATTTCAAATG
  TTACAGATGATTGTAAGCCAAAGCTCTTTCATTTCTCCAAAGAGAGTGCTTATGCCTTACCAACCATGGC
  TTTTTCATTTCTCTGCCATACCTCAATATTGCCCATATACTGTGAACTTCAAAGTCCTTCAAAGAAAAGA
  ATGCAGAATGTTACCAATACAGCAATTGCTTTAAGTTTTCTCATTTATTTTATATCTGCACTCTTTGGGT
  ACCTCACTTTTTATGACAAAGTGGAGTCAGAATTACTAAAAGGTTATAGTAAATACTTATCACATGATGT
  TGTTGTCATGACTGTGAAGTTATGCATACTATTTGCTGTGCTTTTGACAGTCCCTCTAATCCACTTCCCT
  GCCAGAAAAGCTGTAACAATGATGTTTTTCTCCAATTTTCCATTCTCATGGATTCGCCATTTTTTGATCA
  CTCTAGCACTCAATATTATCATCGTTTTACTTGCAATATATGTTCCTGACATTAGAAATGTATTTGGTGT
  AGTTGGTGCCAGTACATCAACATGTTTGATTTTTATATTCCCAGGACTATTTTATCTTAAACTTAGCAGA
  GAGGATTTTCTGTCATGGAAAAAGCTTGGGGCATTCGTTTTGCTCATCTTTGGAATTTTGGTTGGGAATT
  TTAGTTTAGCACTCATCATTTTTGATTGGATTAATAAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG210307 representing NM_153811
  Red=Cloning site Green=Tags(s)
  
  MEASWGSFNAERGWYVSVQQPEEAEAEELSPLLSNELHRQRSPGVSFGLSVFNLMNAIMGSGILGLAYVM
  ANTGVFGFSFLLLTVALLASYSVHLLLSMCIQTAVTSYEDLGLFAFGLPGKLVVAGTIIIQNIGAMSSYL
  LIIKTELPAAIAEFLTGDYSRYWYLDGQTLLIIICVGIVFPLALLPKIGFLGYTSSLSFFFMMFFALVVI
  IKKWSIPCPLTLNYVEKGFQISNVTDDCKPKLFHFSKESAYALPTMAFSFLCHTSILPIYCELQSPSKKR
  MQNVTNTAIALSFLIYFISALFGYLTFYDKVESELLKGYSKYLSHDVVVMTVKLCILFAVLLTVPLIHFP
  ARKAVTMMFFSNFPFSWIRHFLITLALNIIIVLLAIYVPDIRNVFGVVGASTSTCLIFIFPGLFYLKLSR
  EDFLSWKKLGAFVLLIFGILVGNFSLALIIFDWINK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153811
• ORF Size: 1368 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153811.1, NP_722518.1
• RefSeq Size: 1516 bp
• RefSeq ORF: 1371 bp
• Locus ID: 145389
• UniProt ID: Q8IZM9
• Cytogenetics: 14q23.1
• Domains: Aa_trans
• Protein Families: Transmembrane
• Gene Summary: Probable sodium-dependent amino acid/proton antiporter, could be a neuronal transporter for glutamate.[UniProtKB/Swiss-Prot Function]