GBP2 (NM_004120) Human Tagged ORF Clone

CAT#: RG209939

GBP2 (tGFP-tagged) - Human guanylate binding protein 2, interferon-inducible (GBP2)

Product Images

Circular map for RG209939

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: GBP2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG209939 representing NM_004120
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCCAGAGATCAACTTGCCGGGCCCAATGAGCCTCATTGATAACACTAAAGGGCAGCTGGTGGTGA
  ATCCAGAAGCTCTGAAGATCCTATCTGCAATTACGCAGCCTGTGGTGGTGGTGGCGATTGTGGGCCTCTA
  TCGCACAGGCAAATCCTACCTGATGAACAAGCTGGCTGGGAAGAAAAACGGCTTCTCTCTAGGCTCCACA
  GTGAAGTCTCACACCAAGGGAATCTGGATGTGGTGTGTGCCTCATCCCAAGAAGCCAGAACACACCCTAG
  TTCTGCTCGACACTGAGGGCCTGGGAGATATAGAGAAGGGTGACAATGAGAATGACTCCTGGATCTTTGC
  CTTGGCCATCCTCCTGAGCAGCACCTTCGTGTACAATAGCATGGGAACCATCAACCAGCAGGCCATGGAC
  CAACTTCACTATGTGACAGAGCTGACAGATCGAATCAAGGCAAACTCCTCACCTGGTAACAATTCTGTAG
  ACGACTCAGCTGACTTTGTGAGCTTTTTTCCAGCATTTGTGTGGACTCTCAGAGATTTCACCCTGGAACT
  GGAAGTAGATGGAGAACCCATCACTGCTGATGACTACTTGGAGCTTTCGCTAAAGCTAAGAAAAGGTACT
  GATAAGAAAAGTAAAAGCTTTAATGATCCTCGGTTGTGCATCCGAAAGTTCTTCCCCAAGAGGAAGTGCT
  TCGTCTTCGATTGGCCCGCTCCTAAGAAGTACCTTGCTCACCTAGAGCAGCTAAAGGAGGAAGAGCTGAA
  CCCTGATTTCATAGAACAAGTTGCAGAATTTTGTTCCTACATCCTCAGCCATTCCAATGTCAAGACTCTT
  TCAGGTGGCATTCCAGTCAATGGGCCTCGTCTAGAGAGCCTGGTGCTGACCTACGTCAATGCCATCAGCA
  GTGGGGATCTACCCTGCAGGGAGAACGCAGTCCTGGCCTTGGCCCAGATAGAGAACTCAGCCGCAGTGGA
  AAAGGCTATTGCCCACTATGAACAGCAGATGGGCCAGAAGGTGCAGCTGCCCACGGAAACCCTCCAGGAG
  CTGCTGGACCTGCACAGGGACAGTGAGAGAGAGGCCATTGAAGTCTTCATGAAGAACTCTTTCAAGGATG
  TGGACCAAATGTTCCAGAGGAAATTAGGGGCCCAGTTGGAAGCAAGGCGAGATGACTTTTGTAAGCAGAA
  TTCCAAAGCATCATCAGATTGTTGCATGGCTTTACTTCAGGATATATTTGGCCCTTTAGAAGAAGATGTC
  AAGCAGGGAACATTTTCTAAACCAGGAGGTTACCGTCTCTTTACTCAGAAGCTGCAGGAGCTGAAGAATA
  AGTACTACCAGGTGCCAAGGAAGGGGATACAGGCCAAAGAGGTGCTGAAAAAATATTTGGAGTCCAAGGA
  GGATGTGGCTGATGCACTTCTACAGACTGATCAGTCACTCTCAGAAAAGGAAAAAGCGATTGAAGTGGAA
  CGTATAAAGGCTGAATCTGCAGAAGCTGCAAAGAAAATGTTGGAGGAAATACAAAAGAAGAATGAGGAGA
  TGATGGAACAGAAAGAGAAGAGTTATCAGGAACATGTGAAACAATTGACTGAGAAGATGGAGAGGGACAG
  GGCCCAGTTAATGGCAGAGCAAGAGAAGACCCTCGCTCTTAAACTTCAGGAACAGGAACGCCTTCTCAAG
  GAGGGATTCGAGAATGAGAGCAAGAGACTTCAAAAAGACATATGGGATATCCAGATGAGAAGCAAATCAT
  TGGAGCCAATATGTAACATACTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG209939 representing NM_004120
  Red=Cloning site Green=Tags(s)
  
  MAPEINLPGPMSLIDNTKGQLVVNPEALKILSAITQPVVVVAIVGLYRTGKSYLMNKLAGKKNGFSLGST
  VKSHTKGIWMWCVPHPKKPEHTLVLLDTEGLGDIEKGDNENDSWIFALAILLSSTFVYNSMGTINQQAMD
  QLHYVTELTDRIKANSSPGNNSVDDSADFVSFFPAFVWTLRDFTLELEVDGEPITADDYLELSLKLRKGT
  DKKSKSFNDPRLCIRKFFPKRKCFVFDWPAPKKYLAHLEQLKEEELNPDFIEQVAEFCSYILSHSNVKTL
  SGGIPVNGPRLESLVLTYVNAISSGDLPCRENAVLALAQIENSAAVEKAIAHYEQQMGQKVQLPTETLQE
  LLDLHRDSEREAIEVFMKNSFKDVDQMFQRKLGAQLEARRDDFCKQNSKASSDCCMALLQDIFGPLEEDV
  KQGTFSKPGGYRLFTQKLQELKNKYYQVPRKGIQAKEVLKKYLESKEDVADALLQTDQSLSEKEKAIEVE
  RIKAESAEAAKKMLEEIQKKNEEMMEQKEKSYQEHVKQLTEKMERDRAQLMAEQEKTLALKLQEQERLLK
  EGFENESKRLQKDIWDIQMRSKSLEPICNIL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004120
• ORF Size: 1773 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004120.3, NP_004111.2
• RefSeq Size: 2595 bp
• RefSeq ORF: 1776 bp
• Locus ID: 2634
• UniProt ID: P32456
• Cytogenetics: 1p22.2
• Domains: GBP
• Gene Summary: This gene belongs to the guanine-binding protein (GBP) family, which includes interferon-induced proteins that can bind to guanine nucleotides (GMP, GDP and GTP). The encoded protein is a GTPase which hydrolyzes GTP, predominantly to GDP. The protein may play a role as a marker of squamous cell carcinomas. [provided by RefSeq, Jul 2013]