LRRC8E (NM_025061) Human Tagged ORF Clone

CAT#: RG209849

LRRC8E (tGFP-tagged) - Human leucine rich repeat containing 8 family, member E (LRRC8E)

Product Images

Circular map for RG209849

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: LRRC8E
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG209849 representing NM_025061
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCCCAGTGGCCGAGTTCAAGCAGTTCACGGAACAGCAGCCTGCGTTCAAGGTGCTCAAACCCTGGT
  GGGACGTGCTGGCCGAGTACCTCACCGTGGCCATGCTCATGATTGGGGTCTTTGGCTGCACCCTCCAGGT
  GACACAGGACAAGATCATCTGTCTACCCAATCATGAGCTCCAGGAGAACTTATCAGAGGCCCCGTGCCAG
  CAATTGCTGCCTCGGGGGATCCCTGAGCAGATTGGGGCCCTGCAGGAGGTTAAAGGCCTTAAGAACAATT
  TGGACCTGCAGCAATACAGCTTTATTAACCAGCTGTGTTATGAGACGGCCCTGCACTGGTATACCAAGTA
  CTTCCCTTACCTCGTGGTCATTCACACACTCATCTTCATGGTCTGCACCAGTTTCTGGTTCAAGTTCCCT
  GGCACCAGCTCCAAGATTGAACACTTCATCTCCATCCTGGGCAAGTGTTTCGACTCTCCATGGACCACCA
  GGGCCCTATCCGAGGTCTCCGGGGAGAACCAGAAGGGCCCAGCAGCCACCGAACGGGCTGCGGCCACCAT
  AGTGGCCATGGCAGGGACCGGGCCGGGGAAGGCAGGGGAGGGTGAGAAGGAGAAAGTGCTGGCGGAACCG
  GAGAAGGTGGTGACCGAGCCTCCAGTTGTCACCCTGTTGGACAAGAAGGAGGGTGAGCAAGCCAAAGCCC
  TGTTTGAGAAGGTGAAGAAGTTCCGCATGCACGTGGAAGAGGGCGACATCCTGTACACCATGTACATCCG
  ACAGACGGTGCTGAAAGTGTGTAAGTTCCTGGCCATCCTGGTCTACAACCTGGTCTATGTGGAGAAGATC
  AGTTTCCTGGTGGCCTGTAGGGTGGAGACGTCAGAGGTCACGGGCTACGCCAGCTTCTGCTGCAACCACA
  CCAAGGCCCACCTCTTCTCCAAGCTGGCCTTCTGTTACATCTCCTTTGTGTGCATCTACGGACTTACCTG
  CATCTACACGCTCTACTGGCTCTTCCACCGGCCCCTCAAGGAGTACTCCTTCCGTTCCGTGCGGGAGGAG
  ACTGGCATGGGGGACATTCCTGACGTCAAGAATGACTTCGCCTTCATGCTGCACCTCATCGATCAGTACG
  ACTCCCTCTACTCCAAGCGCTTCGCCGTCTTCCTGTCCGAGGTCAGCGAAAGCCGTCTAAAGCAGCTCAA
  TCTCAACCACGAGTGGACCCCCGAGAAGCTTCGACAGAAGCTGCAGCGCAATGCCGCGGGCCGGCTGGAG
  CTGGCCCTCTGCATGCTGCCGGGTCTGCCCGACACCGTCTTTGAGCTCAGTGAGGTGGAGTCACTCAGGC
  TGGAGGCCATCTGCGATATCACCTTCCCCCCGGGGCTGTCACAGCTGGTGCACTTGCAGGAGCTCAGCTT
  GCTCCACTCGCCCGCCAGGCTACCCTTCTCCTTGCAGGTCTTCCTGCGGGACCACCTGAAGGTGATGCGC
  GTCAAATGCGAGGAGCTCCGCGAGGTGCCGCTTTGGGTGTTTGGGCTGCGGGGCTTGGAGGAGCTGCACC
  TGGAGGGGCTTTTCCCCCAGGAGCTAGCTCGGGCAGCCACCCTGGAGAGCCTCCGGGAGCTGAAGCAGCT
  CAAGGTGTTGTCCCTCCGGAGCAACGCCGGGAAGGTGCCAGCCAGTGTGACCGACGTTGCTGGCCACCTG
  CAGAGGCTCAGCCTGCACAACGATGGGGCCCGTCTGGTTGCCCTGAACAGCCTCAAGAAGCTGGCGGCAT
  TGCGGGAGCTGGAGCTGGTGGCCTGCGGGCTGGAGCGCATCCCCCATGCAGTGTTCAGCCTGGGTGCGCT
  GCAGGAACTTGACCTCAAGGACAACCACCTGCGCTCCATCGAGGAAATCCTCAGCTTCCAGCACTGCCGG
  AAGCTGGTCACGCTCAGGCTGTGGCACAACCAGATCGCCTACGTCCCTGAGCACGTGCGGAAGCTCAGGA
  GCCTGGAGCAGCTCTACCTCAGCTACAACAAGCTGGAGACCCTGCCCTCCCAGCTCGGCCTGTGCTCAGG
  CCTCCGTCTGCTGGATGTGTCCCACAATGGGCTACACTCCCTGCCACCCGAGGTGGGCCTCCTGCAGAAC
  CTACAGCACCTGGCCCTCTCCTACAATGCCCTGGAGGCCCTGCCCGAAGAGCTCTTCTTCTGCCGCAAGC
  TGCGGACGTTGCTTCTGGGCGACAACCAGCTGAGCCAGCTCTCGCCCCACGTGGGTGCCCTCAGAGCCCT
  CAGCCGCCTGGAGCTCAAAGGCAACCGCTTAGAGGCGCTGCCAGAAGAACTTGGCAACTGTGGGGGGCTC
  AAGAAGGCGGGGCTCCTGGTGGAAGACACGCTTTACCAGGGTCTGCCGGCAGAAGTGCGGGACAAGATGG
  AGGAGGAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG209849 representing NM_025061
  Red=Cloning site Green=Tags(s)
  
  MIPVAEFKQFTEQQPAFKVLKPWWDVLAEYLTVAMLMIGVFGCTLQVTQDKIICLPNHELQENLSEAPCQ
  QLLPRGIPEQIGALQEVKGLKNNLDLQQYSFINQLCYETALHWYTKYFPYLVVIHTLIFMVCTSFWFKFP
  GTSSKIEHFISILGKCFDSPWTTRALSEVSGENQKGPAATERAAATIVAMAGTGPGKAGEGEKEKVLAEP
  EKVVTEPPVVTLLDKKEGEQAKALFEKVKKFRMHVEEGDILYTMYIRQTVLKVCKFLAILVYNLVYVEKI
  SFLVACRVETSEVTGYASFCCNHTKAHLFSKLAFCYISFVCIYGLTCIYTLYWLFHRPLKEYSFRSVREE
  TGMGDIPDVKNDFAFMLHLIDQYDSLYSKRFAVFLSEVSESRLKQLNLNHEWTPEKLRQKLQRNAAGRLE
  LALCMLPGLPDTVFELSEVESLRLEAICDITFPPGLSQLVHLQELSLLHSPARLPFSLQVFLRDHLKVMR
  VKCEELREVPLWVFGLRGLEELHLEGLFPQELARAATLESLRELKQLKVLSLRSNAGKVPASVTDVAGHL
  QRLSLHNDGARLVALNSLKKLAALRELELVACGLERIPHAVFSLGALQELDLKDNHLRSIEEILSFQHCR
  KLVTLRLWHNQIAYVPEHVRKLRSLEQLYLSYNKLETLPSQLGLCSGLRLLDVSHNGLHSLPPEVGLLQN
  LQHLALSYNALEALPEELFFCRKLRTLLLGDNQLSQLSPHVGALRALSRLELKGNRLEALPEELGNCGGL
  KKAGLLVEDTLYQGLPAEVRDKMEEE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025061
• ORF Size: 2388 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025061.3, NP_079337.2
• RefSeq Size: 3625 bp
• RefSeq ORF: 2391 bp
• Locus ID: 80131
• UniProt ID: Q6NSJ5
• Cytogenetics: 19p13.2
• Domains: LRR
• Protein Families: Transmembrane
• Gene Summary: This gene encodes a member of a small, conserved family of proteins with similar structure, including a string of extracellular leucine-rich repeats. A related protein was shown to be involved in B-cell development. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jun 2012]