GPR75 (NM_006794) Human Tagged ORF Clone

CAT#: RG209675

GPR75 (tGFP-tagged) - Human G protein-coupled receptor 75 (GPR75)

Product Images

Circular map for RG209675

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: GPR75
• Synonyms: GPRchr2; WI31133
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG209675 representing NM_006794
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACTCAACAGGCCACCTTCAGGATGCCCCCAATGCCACCTCGCTCCATGTGCCTCACTCACAGGAAG
  GAAACAGCACCTCTCTCCAGGAGGGTCTTCAGGATCTCATCCACACAGCCACCTTGGTGACCTGTACTTT
  TCTACTGGCGGTCATCTTCTGCCTGGGTTCCTATGGCAACTTCATTGTCTTCTTGTCCTTCTTCGATCCA
  GCCTTCAGGAAATTCAGAACCAACTTTGATTTCATGATCCTGAACCTGTCCTTCTGTGACCTCTTCATTT
  GTGGAGTGACAGCCCCCATGTTCACCTTTGTGTTATTCTTCAGCTCAGCCAGTAGTATCCCGGATGCTTT
  CTGCTTCACTTTCCATCTCACCAGTTCAGGCTTCATCATCATGTCTCTGAAGACAGTGGCAGTGATCGCC
  CTGCACCGGCTCCGGATGGTGTTGGGGAAACAGCCTAATCGCACGGCCTCCTTTCCCTGCACCGTACTCC
  TCACCCTGCTTCTCTGGGCCACCAGTTTCACCCCTGCCACCTTGGCTACCTTGAAAACCAGCAAGTCCCA
  CCTCTGTCTTCCCATGTCCAGTCTGATTGCTGGAAAAGGGAAAGCCATTTTGTCTCTCTATGTGGTCGAC
  TTCACCTTCTGTGTTGCTGTGGTCTCTGTCTCTTACATCATGATTGCTCAGACCCTGCGGAAGAACGCTC
  AAGTCAGAAAGTGCCCCCCTGTAATCACAGTCGATGCTTCCAGACCACAGCCTTTCATGGGGGTCCCTGT
  GCAGGGAGGTGGAGATCCCATCCAGTGTGCCATGCCGGCTCTGTATAGGAACCAGAATTACGACAAACTG
  CAGCACGTTCAGACCCGTGGATATACCAAGAGTCCCAACCAACTGGTCACCCCTGCAGCAAGCCGACTCC
  AGCTCGTATCAGCCATCAACCTCTCCACTGCCAAGGATTCCAAAGCCGTGGTCACCTGTGTGATCATTGT
  GCTGTCAGTCCTGGTGTGCTGTCTTCCACTGGGGATTTCCTTGGTACAGGTGGTTCTCTCCAGCAATGGG
  AGCTTCATTCTTTACCAGTTTGAATTGTTTGGATTTACTCTTATATTTTTCAAGTCAGGATTAAACCCTT
  TTATATATTCTCGGAACAGTGCAGGGCTGAGAAGGAAAGTGCTCTGGTGCCTCCAATACATAGGCCTGGG
  TTTTTTCTGCTGCAAACAAAAGACTCGACTTCGAGCCATGGGAAAAGGGAACCTCGAAGTCAACAGAAAC
  AAATCCTCCCATCATGAAACAAACTCTGCCTACATGTTATCTCCAAAGCCACAGAAGAAATTTGTGGACC
  AGGCTTGTGGCCCAAGTCATTCAAAAGAAAGTATGGTGAGTCCCAAGATCTCTGCTGGACATCAACACTG
  TGGTCAGAGCAGCTCGACCCCCATCAACACTCGGATTGAACCTTACTACAGCATCTATAACAGCAGCCCT
  TCCCAGGAGGAGAGCAGCCCATGTAACTTACAGCCAGTAAACTCTTTTGGATTTGCCAATTCATATATTG
  CCATGCATTATCACACCACTAATGACTTAGTGCAGGAATATGACAGCACTTCAGCCAAGCAGATTCCAGT
  CCCCTCCGTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG209675 representing NM_006794
  Red=Cloning site Green=Tags(s)
  
  MNSTGHLQDAPNATSLHVPHSQEGNSTSLQEGLQDLIHTATLVTCTFLLAVIFCLGSYGNFIVFLSFFDP
  AFRKFRTNFDFMILNLSFCDLFICGVTAPMFTFVLFFSSASSIPDAFCFTFHLTSSGFIIMSLKTVAVIA
  LHRLRMVLGKQPNRTASFPCTVLLTLLLWATSFTPATLATLKTSKSHLCLPMSSLIAGKGKAILSLYVVD
  FTFCVAVVSVSYIMIAQTLRKNAQVRKCPPVITVDASRPQPFMGVPVQGGGDPIQCAMPALYRNQNYDKL
  QHVQTRGYTKSPNQLVTPAASRLQLVSAINLSTAKDSKAVVTCVIIVLSVLVCCLPLGISLVQVVLSSNG
  SFILYQFELFGFTLIFFKSGLNPFIYSRNSAGLRRKVLWCLQYIGLGFFCCKQKTRLRAMGKGNLEVNRN
  KSSHHETNSAYMLSPKPQKKFVDQACGPSHSKESMVSPKISAGHQHCGQSSSTPINTRIEPYYSIYNSSP
  SQEESSPCNLQPVNSFGFANSYIAMHYHTTNDLVQEYDSTSAKQIPVPSV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006794
• ORF Size: 1620 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006794.1, NP_006785.1
• RefSeq Size: 2054 bp
• RefSeq ORF: 1623 bp
• Locus ID: 10936
• UniProt ID: O95800
• Cytogenetics: 2p16.2
• Domains: 7tm_1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Gene Summary: GPR75 is a member of the G protein-coupled receptor family. GPRs are cell surface receptors that activate guanine-nucleotide binding proteins upon the binding of a ligand.[supplied by OMIM, Jul 2002]