FAM82A2 (RMDN3) (NM_018145) Human Tagged ORF Clone

CAT#: RG209526

RMDN3 (tGFP-tagged) - Human family with sequence similarity 82, member A2 (FAM82A2)

Product Images

Circular map for RG209526

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: FAM82A2
• Synonyms: FAM82A2; FAM82C; ptpip51; RMD-3; RMD3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG209526 representing NM_018145
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTAGACTGGGAGCCCTGGGTGGTGCCCGTGCCGGGCTGGGACTGTTGCTGGGTACCGCCGCCGGCC
  TTGGATTCCTGTGCCTCCTTTACAGCCAGCGATGGAAACGGACCCAGCGTCATGGCCGCAGCCAGAGCCT
  GCCCAACTCCCTGGACTATACGCAGACTTCAGATCCCGGACGCCACGTGATGCTCCTGCGGGCTGTCCCA
  GGTGGGGCTGGAGATGCCTCAGTGCTGCCCAGCCTTCCACGGGAAGGACAGGAGAAGGTGCTGGACCGCC
  TGGACTTTGTGCTGACCAGCCTTGTGGCGCTGCGGCGGGAGGTGGAGGAGCTGAGAAGCAGCCTGCGAGG
  GCTTGCGGGGGAGATTGTTGGGGAGGTCCGATGCCACATGGAAGAGAACCAGAGAGTGGCTCGGCGGCGA
  AGGTTTCCGTTTGTCCGGGAGAGGAGTGACTCCACTGGCTCCAGCTCTGTCTACTTCACGGCCTCCTCGG
  GAGCCACGTTCACAGATGCTGAGAGTGAAGGGGGTTACACAACAGCCAATGCGGAGTCTGACAATGAGCG
  GGACTCTGACAAAGAAAGTGAGGACGGGGAAGATGAAGTGAGCTGTGAGACTGTGAAGATGGGGAGAAAG
  GATTCTCTTGACTTGGAGGAAGAGGCAGCTTCAGGTGCCTCCAGTGCCCTGGAGGCTGGAGGTTCCTCAG
  GCTTGGAGGATGTGCTGCCCCTCCTGCAGCAGGCCGACGAGCTGCACAGGGGTGATGAGCAAGGCAAGCG
  GGAGGGCTTCCAGCTGCTGCTCAACAACAAGCTGGTGTATGGAAGCCGGCAGGACTTTCTCTGGCGCCTG
  GCCCGAGCCTACAGTGACATGTGTGAGCTCACTGAGGAGGTGAGCGAGAAGAAGTCATATGCCCTAGATG
  GAAAAGAAGAAGCAGAGGCTGCTCTGGAGAAGGGGGATGAGAGTGCTGACTGTCACCTGTGGTATGCGGT
  GCTTTGTGGTCAGCTGGCTGAGCATGAGAGCATCCAGAGGCGCATCCAGAGTGGCTTTAGCTTCAAGGAG
  CATGTGGACAAAGCCATTGCTCTCCAGCCAGAAAACCCCATGGCTCACTTTCTTCTTGGCAGGTGGTGCT
  ATCAGGTCTCTCACCTGAGCTGGCTAGAAAAAAAAACTGCTACAGCCTTGCTTGAAAGCCCTCTCAGTGC
  CACTGTGGAAGATGCCCTCCAGAGCTTCCTAAAGGCTGAAGAACTACAGCCAGGATTTTCCAAAGCAGGA
  AGGGTATATATTTCCAAGTGCTACAGAGAACTAGGGAAAAACTCTGAAGCTAGATGGTGGATGAAGTTGG
  CCCTGGAGCTGCCAGATGTCACGAAGGAGGATTTGGCTATCCAGAAGGACCTGGAAGAACTGGAAGTCAT
  TTTACGAGAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG209526 representing NM_018145
  Red=Cloning site Green=Tags(s)
  
  MSRLGALGGARAGLGLLLGTAAGLGFLCLLYSQRWKRTQRHGRSQSLPNSLDYTQTSDPGRHVMLLRAVP
  GGAGDASVLPSLPREGQEKVLDRLDFVLTSLVALRREVEELRSSLRGLAGEIVGEVRCHMEENQRVARRR
  RFPFVRERSDSTGSSSVYFTASSGATFTDAESEGGYTTANAESDNERDSDKESEDGEDEVSCETVKMGRK
  DSLDLEEEAASGASSALEAGGSSGLEDVLPLLQQADELHRGDEQGKREGFQLLLNNKLVYGSRQDFLWRL
  ARAYSDMCELTEEVSEKKSYALDGKEEAEAALEKGDESADCHLWYAVLCGQLAEHESIQRRIQSGFSFKE
  HVDKAIALQPENPMAHFLLGRWCYQVSHLSWLEKKTATALLESPLSATVEDALQSFLKAEELQPGFSKAG
  RVYISKCYRELGKNSEARWWMKLALELPDVTKEDLAIQKDLEELEVILRD
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018145
• ORF Size: 1410 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018145.3
• RefSeq Size: 2251 bp
• RefSeq ORF: 1413 bp
• Locus ID: 55177
• UniProt ID: Q96TC7
• Cytogenetics: 15q15.1
• Protein Families: Transmembrane
• Gene Summary: Involved in cellular calcium homeostasis regulation. May participate in differentiation and apoptosis of keratinocytes. Overexpression induces apoptosis.[UniProtKB/Swiss-Prot Function]