RHOJ (NM_020663) Human Tagged ORF Clone

CAT#: RG209395

RHOJ (tGFP-tagged) - Human ras homolog gene family, member J (RHOJ)

Product Images

Circular map for RG209395

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: RHOJ
• Synonyms: ARHJ; RASL7B; TC10B; TCL
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG209395 representing NM_020663
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACTGCAAAGAGGGAACTGACAGCAGCTGCGGCTGCAGGGGCAACGACGAGAAGAAGATGTTGAAGT
  GTGTGGTGGTGGGGGACGGTGCCGTGGGGAAAACCTGCCTGCTGATGAGCTACGCCAACGACGCCTTCCC
  AGAGGAATACGTGCCCACTGTGTTTGACCACTATGCAGTTACTGTGACTGTGGGAGGCAAGCAACACTTG
  CTCGGACTGTATGACACCGCGGGACAGGAGGACTACAACCAGCTGAGGCCACTCTCCTACCCCAACACGG
  ATGTGTTTTTGATCTGCTTCTCTGTCGTAAACCCTGCCTCTTACCACAATGTCCAGGAGGAATGGGTCCC
  CGAGCTCAAGGACTGCATGCCTCACGTGCCTTATGTCCTCATAGGGACCCAGATTGATCTCCGTGATGAC
  CCAAAAACCTTGGCCCGTTTGCTGTATATGAAAGAGAAACCTCTCACTTACGAGCATGGTGTGAAGCTCG
  CAAAAGCGATCGGAGCACAGTGCTACTTGGAATGTTCAGCTCTGACTCAGAAAGGTCTCAAAGCGGTTTT
  TGATGAAGCAATCCTCACCATTTTCCACCCCAAGAAAAAGAAGAAACGCTGTTCTGAGGGTCACAGCTGC
  TGTTCAATTATC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG209395 representing NM_020663
  Red=Cloning site Green=Tags(s)
  
  MNCKEGTDSSCGCRGNDEKKMLKCVVVGDGAVGKTCLLMSYANDAFPEEYVPTVFDHYAVTVTVGGKQHL
  LGLYDTAGQEDYNQLRPLSYPNTDVFLICFSVVNPASYHNVQEEWVPELKDCMPHVPYVLIGTQIDLRDD
  PKTLARLLYMKEKPLTYEHGVKLAKAIGAQCYLECSALTQKGLKAVFDEAILTIFHPKKKKKRCSEGHSC
  CSII
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020663
• ORF Size: 642 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020663.5
• RefSeq Size: 1907 bp
• RefSeq ORF: 645 bp
• Locus ID: 57381
• UniProt ID: Q9H4E5
• Cytogenetics: 14q23.2
• Domains: ras, RAS, RHO, RAB
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes one of the many small GTP-binding proteins in the Rho family shown to be associated with focal adhesions in endothelial cells (PMID: 21148427, 22103495). The encoded protein is activated by vascular endothelial growth factor and may regulate angiogenesis. [provided by RefSeq, Dec 2011]