PRMT3 (NM_005788) Human Tagged ORF Clone

CAT#: RG209382

PRMT3 (tGFP-tagged) - Human protein arginine methyltransferase 3 (PRMT3), transcript variant 1

Product Images

Circular map for RG209382

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PRMT3
• Synonyms: HRMT1L3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG209382 representing NM_005788
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGCTCGTTAGCGTCAGGCGCTACCGGCGGCCGGGGCGCTGTGGAGAATGAGGAGGACCTGCCAGAAC
  TGTCGGACAGCGGGGACGAGGCCGCCTGGGAGGATGAGGACGATGCAGATCTCCCCCACGGCAAGCAGCA
  GACCCCCTGCCTGTTCTGTAACAGGTTATTCACATCTGCTGAAGAAACATTTTCACACTGTAAGTCTGAG
  CATCAGTTTAATATTGACAGCATGGTTCATAAACATGGACTTGAATTTTATGGATACATTAAGCTAATAA
  ATTTTATTAGACTTAAGAATCCTACAGTTGAGTACATGAATTCCATATACAACCCAGTGCCTTGGGAGAA
  AGAAGAGTATTTGAAGCCAGTATTAGAAGATGACCTTTTACTTCAATTTGATGTAGAAGATCTTTATGAA
  CCGGTGTCAGTACCCTTCTCATACCCCAATGGACTCAGTGAAAATACATCTGTTGTTGAAAAATTGAAAC
  ATATGGAAGCCAGGGCACTGTCTGCTGAAGCCGCATTGGCCAGAGCACGTGAGGATCTGCAAAAAATGAA
  ACAATTTGCTCAGGATTTTGTGATGCACACAGATGTCAGAACCTGCTCGTCATCTACTAGTGTCATTGCG
  GACCTCCAGGAGGATGAGGATGGTGTTTATTTCAGCTCATACGGGCATTATGGGATACATGAAGAAATGC
  TAAAGGACAAAATACGAACAGAAAGCTACCGAGATTTCATATACCAAAATCCACATATCTTCAAAGACAA
  GGTAGTTTTGGATGTTGGGTGTGGAACTGGAATTCTCTCTATGTTTGCTGCTAAAGCTGGGGCGAAGAAG
  GTTCTTGGAGTTGATCAATCTGAAATACTTTACCAGGCAATGGATATTATAAGACTAAATAAACTTGAAG
  ATACTATTACACTAATTAAAGGAAAGATTGAAGAAGTTCATCTTCCTGTAGAAAAAGTAGATGTTATCAT
  ATCTGAGTGGATGGGCTATTTTCTTCTGTTTGAGTCTATGTTAGATTCTGTCCTTTATGCAAAGAACAAA
  TACTTGGCAAAAGGAGGCTCGGTCTACCCTGACATTTGCACTATCAGCCTTGTAGCAGTGAGTGATGTGA
  ATAAACATGCTGATAGAATTGCTTTTTGGGATGATGTCTATGGCTTCAAGATGTCCTGCATGAAGAAAGC
  AGTTATTCCAGAAGCTGTTGTGGAAGTTTTAGATCCGAAGACTCTTATTTCAGAACCTTGTGGTATTAAG
  CATATAGATTGCCATACGACGTCTATCTCAGATTTGGAATTTTCATCAGATTTTACCCTGAAAATCACAA
  GGACATCCATGTGCACGGCAATTGCTGGCTACTTTGATATATATTTTGAGAAGAATTGCCACAACAGGGT
  CGTGTTCTCTACGGGCCCTCAGAGCACCAAAACACACTGGAAACAAACAGTATTTCTACTGGAAAAACCA
  TTTTCAGTTAAAGCAGGTGAAGCCTTGAAAGGAAAGGTCACAGTTCACAAGAATAAGAAAGATCCACGTT
  CTCTCACCGTGACCCTCACGTTGAATAATTCAACTCAAACTTATGGTCTCCAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG209382 representing NM_005788
  Red=Cloning site Green=Tags(s)
  
  MCSLASGATGGRGAVENEEDLPELSDSGDEAAWEDEDDADLPHGKQQTPCLFCNRLFTSAEETFSHCKSE
  HQFNIDSMVHKHGLEFYGYIKLINFIRLKNPTVEYMNSIYNPVPWEKEEYLKPVLEDDLLLQFDVEDLYE
  PVSVPFSYPNGLSENTSVVEKLKHMEARALSAEAALARAREDLQKMKQFAQDFVMHTDVRTCSSSTSVIA
  DLQEDEDGVYFSSYGHYGIHEEMLKDKIRTESYRDFIYQNPHIFKDKVVLDVGCGTGILSMFAAKAGAKK
  VLGVDQSEILYQAMDIIRLNKLEDTITLIKGKIEEVHLPVEKVDVIISEWMGYFLLFESMLDSVLYAKNK
  YLAKGGSVYPDICTISLVAVSDVNKHADRIAFWDDVYGFKMSCMKKAVIPEAVVEVLDPKTLISEPCGIK
  HIDCHTTSISDLEFSSDFTLKITRTSMCTAIAGYFDIYFEKNCHNRVVFSTGPQSTKTHWKQTVFLLEKP
  FSVKAGEALKGKVTVHKNKKDPRSLTVTLTLNNSTQTYGLQ
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005788
• ORF Size: 1593 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005788.4
• RefSeq Size: 2632 bp
• RefSeq ORF: 1596 bp
• Locus ID: 10196
• UniProt ID: O60678
• Cytogenetics: 11p15.1
• Protein Families: Druggable Genome
• Gene Summary: This gene belongs to the protein arginine methyltransferase (PRMT) family. The encoded enzyme catalyzes the methylation of guanidino nitrogens of arginyl residues of proteins. The enzyme acts on 40S ribosomal protein S2 (rpS2), which is its major in-vivo substrate, and is involved in the proper maturation of the 80S ribosome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]